His Tag Antibody (His17) - BSA Free

Western Blot: His Tag Antibody (His17) [NB100-72978] - Human recombinant protein with HRP conjugated His-tag. Western blot image submitted by a verified customer review.

Simple Western: His Tag Antibody (His17) [NB100-72978] - Human purified protein. Simple Western image submitted by a verified customer review.

• Reactivity: ET
• Applications: WB, Simple Western, ELISA, IP
• Clone: His17
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

His Tag Antibody (His17) - BSA Free Summary

• Description: Novus Biologicals Mouse His Tag Antibody (His17) - BSA Free (NB100-72978) is a monoclonal antibody validated for use in WB, ELISA, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with His6-Tag conjugated to BSA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western
  • Western Blot 1:100 - 1:2000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Human purified protein, separated by Size

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for His Tag Antibody (His17) - BSA Free

• 6 His epitope tag
• 6-His Tag
• 6X His Tag
• 6X His
• 6x-His Tag
• 6X-His
• H
• Hexa His tag
• HHHHHH epitope tag
• HHHHHH tag
• His Tag
• HIS
• His6-Tag
• polyhistidine tag
• polyHistidine
• Poly-histidine

Background

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for His Tag Antibody (NB100-72978) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 12/04/2018

Summary

• Application: Simple Western
• Sample Tested: Purified protein
• Species: Human
• Lot: 17/12-H1-His17

FAQs for His Tag Antibody (NB100-72978). (Showing 1 - 1 of 1 FAQ).

1. Is this really against the HIS-tag of his-tagged proteins? The specificity and immunogen mentions a mouse monoclonal - not HIS-tag. Please, confirm the immunogen for this antibody.
   • NB100-72978 is raised against His tag and is suitable to detect any His tagged protein.