His Tag Antibody (His17) [HRP]

Western Blot: His Tag Antibody (His17) [HRP] [NB100-72978H] - Human recombinant protein with HRP conjugated His-tag. Western blot image submitted by a verified customer review.

• Reactivity: ET
• Applications: WB, ELISA, IP
• Clone: His17
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

His Tag Antibody (His17) [HRP] Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with His6-Tag conjugated to BSA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

6x His Tag specific

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for His Tag Antibody (His17) [HRP]

• 6 His epitope tag
• 6-His Tag
• 6X His Tag
• 6X His
• 6x-His Tag
• 6X-His
• H
• Hexa His tag
• HHHHHH epitope tag
• HHHHHH tag
• His Tag
• HIS
• His6-Tag
• polyhistidine tag
• polyHistidine
• Poly-histidine

Background

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for His Tag Antibody (NB100-72978H) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Human and recombinant protein with his-tag.

reviewed by: Verified Customer ELISA Mouse 10/27/2020

Summary

• Application: ELISA
• Sample Tested: Human IgG
• Species: Mouse
• Lot: 20/07--H1-His17-102320-H

reviewed by: Verified Customer WB Human and recombinant protein with his-tag 04/05/2019

Summary

• Application: Western Blot
• Sample Tested: recombinant protein with his-tag
• Species: Human and recombinant protein with his-tag
• Lot: 17/12-H1-His17-030118-1