His Tag Antibody (HIS.H8)

Images

 
Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the HRP conjugate of NBP2-31055. Detection of 6x-His Epitope Tag was performed by loading ...read more
Immunocytochemistry/ Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 550 conjugate of NBP2-31055. Staining of HeLa cells ...read more
Western Blot: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.
Western Blot: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of His-tagged protein in E. coli lysates. Data courtesy of the Innovators Program.
Western Blot: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector ...read more
Western Blot: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different ...read more
Western Blot: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of recombinant Treponema denticola major outer sheath His-tagged protein fragments (rMSP, rMSP-C, rMSP-V, rMSP-N) purified from E. coli was ...read more
Western Blot: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of 15ug of cell lysates from SF9 cells expressing an 11kD 6x-His tagged protein per well onto an SDS-PAGE gel.
Western Blot: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 680 conjugate of NBP2-31055. Detection of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate ...read more
Immunocytochemistry/ Immunofluorescence: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained ...read more
Immunocytochemistry/ Immunofluorescence: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 650 conjugate of NBP2-31055. Staining of HeLa cells transfected with a construct containing a ...read more
Immunocytochemistry/ Immunofluorescence: 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 680 conjugate of NBP2-31055. Staining of HeLa cells transfected with a construct containing a ...read more
Immunocytochemistry/ Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 488 conjugate of NBP2-31055. Staining of HeLa cells ...read more

Product Details

Summary
Reactivity Hu, NA, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP
Clone
HIS.H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

His Tag Antibody (HIS.H8) Summary

Immunogen
6x His synthetic peptide.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:3000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2000
  • Immunoprecipitation 1:10 - 1:500

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25353373). Sheep reactivity reported in scientific literature (PMID: 25644118).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for His Tag Antibody (HIS.H8)

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-31055AF405
Alexa Fluor 488 Labeled NBP2-31055AF488
Alexa Fluor 647 Labeled NBP2-31055AF647
Alexa Fluor 700 Labeled NBP2-31055AF700
DyLight 488 Labeled NBP2-31055G
DyLight 550 Labeled NBP2-31055R
DyLight 650 Labeled NBP2-31055C
DyLight 680 Labeled NBP2-31055FR
HRP Labeled NBP2-31055H

Additional His Tag Products

His Tag NBP2-31055

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