His Tag Antibody (HIS.H8) Summary
6x His synthetic peptide.
Protein A purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:1000 - 1:3000
- ELISA 1:100 - 1:2000
- Flow Cytometry 1:10-1:1000
- Immunocytochemistry/Immunofluorescence 1:500 - 1:2000
- Immunoprecipitation 1:10 - 1:500
Human reactivity reported in scientific literature (PMID: 25353373). Sheep reactivity reported in scientific literature (PMID: 25644118).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
Protein A purified
Alternate Names for His Tag Antibody (HIS.H8)
- 6 His epitope tag
- 6X His
- HHHHHH epitope tag
- HHHHHH tag
- His Tag
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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