DYKDDDDK Epitope Tag Antibody (29E4.G7)

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Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Twenty-four clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was ...read more
Immunohistochemistry-Paraffin: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Analysis using the Biotin conjugate of NBP1-97410. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) ...read more
Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - WB on 15ug HeLa whole cell lysate. Image from verified customer review.
Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Monoclonal Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins.
Immunohistochemistry-Paraffin: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Analysis using the Biotin conjugate of NBP1-97410. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to ...read more
Immunohistochemistry-Paraffin: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Analysis using the Biotin conjugate of NBP1-97410. Tissue: human lymph nodes at pH9. Fixation: formalin fixed paraffin embedded. ...read more
ELISA: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Analysis using the DyLight 405 conjugate of NBP1-97410. Detection of antibody with FLAG conjugated proteins.

Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
29E4.G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

DYKDDDDK Epitope Tag Antibody (29E4.G7) Summary

Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added
Specificity
The purified antibody is directed against the motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal tag antibody detects over-expressed proteins containing the epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:20000
  • ELISA 1:20000-1:100000
  • Flow Cytometry 1:1:10 - 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody is optimally suited for monitoring the expression of FLAG tagged fusion proteins. As such, this antibody can be used - identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused - either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-97410 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for DYKDDDDK Epitope Tag Antibody (29E4.G7)

  • DDDDK epitope tag
  • DDK
  • DYKDDDDK Epitope Tag
  • DYKDDDDK tag
  • ECS epitope tag
  • ECS tag
  • Enterokinase Cleavage Site epitope tag
  • Enterokinase Cleavage Site tag
  • epitope tag flag
  • FLAG (FLAG is a trademark of Sigma Aldrich)
  • FLAG Tag
  • Ty 1 Tag
  • xxxDDDDK epitope tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DYKDDDDK Epitope Tag Antibody (NBP1-97410) (0)

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Reviews for DYKDDDDK Epitope Tag Antibody (NBP1-97410) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: Simple Western, WB.

Reviews using NBP1-97410:
Filter by Applications
Simple Western
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Simple Western Human 08/20/2015
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Summary

ApplicationSimple Western
Sample TestedHEK 293 cell lysate
SpeciesHuman

Blocking

Blocking Details5 % Non-fat milk for 20 minutes at RT

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGOAT ANTI-mouse igG, HRP
Secondary Concentration1:10000

Details

Detection Noteschemiluminescent, GE 4000, 30sec
Western Blot DYKDDDDK Epitope Tag NBP1-97410
Enlarge
reviewed by:
Grace zhou
WB Human 07/30/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% Non fat milk in TBS, 20 minutes at RT

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptiondonkey anti-mouse IgG, HRP
Secondary Concentration1:1000

Details

Detection Noteschemiluminescent, LAS 4000, 30second

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DYKDDDDK Epitope Tag Antibody (NBP1-97410). (Showing 1 - 7 of 7 FAQ).

  1. Are the FLAG Tag antibodies validated in Simple Western?
    • Yes, we offer a 2 FLAG Tag antibodies that have been tested in Simple Western: NB600-345 and NBP1-71705.
  2. Does FLAG Tag antibodies comes in lyophilized form?
    • The following FLAG Tag antibodies come in lyophilized form: NBP1-97401, NBP1-97399, NBP1-97400, NBP1-97402.
  3. What the theoretical molecular weight for FLAG Tag antibodies?
    • The TMW of FLAG Tag antibodies is approximately 150 kDa.
  4. Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
    • If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
  5. Will FLAG Tag antibodies recognizes FLAG at the C-terminal end?
    • Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
  6. Hello. We are planning to promote DYKDDDDK Antibodies in our February 2013 newsletter. Thus, we would like to check toxicity of a few products. Could you let us know whether these are lyophilized form?
    • NBP1-97410 does not come in lyophilized form.
  7. What is the immunogen sequence of this FLAG Tag antibody?
    • A synthetic peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) corresponding to the FLAG epitope tag peptide DYKDDDDK, conjugated to KLH.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-97400
DyLight 405 Labeled NBP1-97399
Fluorescein Labeled NBP1-97401
HRP Labeled NBP1-97393

Additional DYKDDDDK Epitope Tag Products

DYKDDDDK Epitope Tag NBP1-97410

Blogs on DYKDDDDK Epitope Tag.

Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research...  Read full blog post.

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Recent Reviews

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Anonymous
08/20/2015
Application: Simple Western
Species: Human

Grace zhou
07/30/2014
Application: WB
Species: Human