His Tag Antibody (HIS.H8) [Alexa Fluor® 532]

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Product Details

Summary
Reactivity Hu, All-NA, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
HIS.H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 532

Order Details

His Tag Antibody (HIS.H8) [Alexa Fluor® 532] Summary

Immunogen
6x His synthetic peptide.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25353373). Sheep reactivity reported in scientific literature (PMID: 25644118).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for His Tag Antibody (HIS.H8) [Alexa Fluor® 532]

  • 6 His epitope tag
  • 6-His Tag
  • 6X His Tag
  • 6X His
  • 6x-His Tag
  • 6X-His
  • H
  • Hexa His tag
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • His6-Tag
  • polyhistidine tag
  • polyHistidine
  • Poly-histidine

Background

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for His Tag Antibody (NBP2-31055AF532) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-31055AF350
Alexa Fluor 405 NBP2-31055AF405
Alexa Fluor 488 NBP2-31055AF488
Alexa Fluor 532 NBP2-31055AF532
Alexa Fluor 594 NBP2-31055AF594
Alexa Fluor 647 NBP2-31055AF647
Alexa Fluor 700 NBP2-31055AF700
Alexa Fluor 750 NBP2-31055AF750
Allophycocyanin NBP2-31055APC
Allophycocyanin/Cy7 NBP2-31055APCCY7
Biotin NBP2-31055B
DyLight 350 NBP2-31055UV
DyLight 405 NBP2-31055V
DyLight 488 NBP2-31055G
DyLight 550 NBP2-31055R
DyLight 594 NBP2-31055DL594
DyLight 650 NBP2-31055C
DyLight 680 NBP2-31055FR
DyLight 755 NBP2-31055IR
FITC NBP2-31055F
HRP NBP2-31055H
Janelia Fluor 549 NBP2-31055JF549
Janelia Fluor 646 NBP2-31055JF646
PE NBP2-31055PE
PE/Atto594 NBP2-31055PEATT594
PE/Cy5.5 NBP2-31055PECY55
PE/Cy7 NBP2-31055PECY7
PerCP NBP2-31055PCP

Additional His Tag Products

Array NBP2-31055AF532

Research Areas for His Tag Antibody (NBP2-31055AF532)

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Contact Information

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Calculators

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