Paramyxovirus SV5 Pk Antibody (SV5-Pk1) - BSA Free

Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - Analysis using the Biotin conjugate of NB100-62264. Detection of V5 tagged protein with Mouse anti V5-tag.

Sandwich ELISA: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - V5 tagged protein detected with Mouse anti V5-Tag.

• Reactivity: V
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IP
• Clone: SV5-Pk1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Paramyxovirus SV5 Pk Antibody (SV5-Pk1) - BSA Free Summary

• Immunogen: Paramyxovirus Simian-Virus 5 (SV5)
• Specificity: This product recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NB100-62264 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro (ile pro asn pro leu leu gly leu asp) ser thr. (9 amino acid epitope is in parenthesis)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000
  • Radioimmunoassay 1:100-1:2000
  • Sandwich ELISA
  • Western Blot 1:1000-1:5000

Reactivity Notes

Epitope tag. Non-species specific. Mouse reactivity reported in scientific literature (PMID: 27681617).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Paramyxovirus SV5 Pk Antibody (SV5-Pk1) - BSA Free

• Simian virus 5; SPV5gp2; V protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Paramyxovirus SV5 Pk Antibody (NB100-62264)(30)

Publications using NB100-62264

• Muntean M, Briciu V, Lupse M et al. Effects of COVID-19 on the Liver and Mortality in Patients with SARS-CoV-2 Pneumonia Caused by Delta and Non-Delta Variants: An Analysis in a Single Centre Pharmaceuticals 2023-12-19 [PMID: 38275989]
• Fu J, Zhu J, Du F et al. Prognostic Inflammatory Index Based on Preoperative Peripheral Blood for Predicting the Prognosis of Colorectal Cancer Patients Cancers 2020-12-22 [PMID: 33374924]
• Gebbia V, Aiello MM, Banna G et al. Metronomic oral vinorelbine in patients with advanced non-small cell lung cancer progressing after nivolumab immunotherapy: a retrospective analysis ecancermedicalscience 2020-09-29 [PMID: 33144881]
• Rao D, Ferguson R, Sikora M. WNT4 and WNT3A activate cell autonomous Wnt signaling independent of secretion J Biol Chem 2019-11-20 [PMID: 31740580]
• Wang M, Mei Y, Ryde U. Predicting Relative Binding Affinity Using Nonequilibrium QM/MM Simulations. Journal of chemical theory and computation 2019-04-22 [PMID: 30362750]
• da Silva JNL, Ranzi AD, Carvalho CT et al. Cell Cycle Markers in the Evaluation of Bladder Cancer. Pathology oncology research : POR 2020-12-11 [PMID: 29524168]
• Schunck RVA, Macedo IC, Laste G et Al. Standardized Passiflora incarnata L. Extract Reverts the Analgesia Induced by Alcohol Withdrawal in Rats Phytother Res 2017-10-03 [PMID: 28568647]
• Yang C, Danielson EW, Qiao T et al. Mutant PFN1 causes ALS phenotypes and progressive motor neuron degeneration in mice by a gain of toxicity. Proc. Natl. Acad. Sci. U.S.A. 2016-10-11 [PMID: 27681617] (ICC/IF, IF/IHC, Mouse)
• Walter D, Hoffmann S, Komseli ES et al. SCF(Cyclin F)-dependent degradation of CDC6 suppresses DNA re-replication. Nat Commun. 2016-01-28 [PMID: 26818844] (ICC/IF, WB)
• Ghosh S, Wu MD, Shaftel SS et al. Sustained interleukin-1beta overexpression exacerbates tau pathology despite reduced amyloid burden in an Alzheimer's mouse model. J Neurosci 2013-03-13 [PMID: 23486975] (ICC/IF, IHC-Fr)
• Gatherer, D et al. High-resolution human cytomegalovirus transcriptome. Proc Natl Acad Sci U S A. 2011-11-22 [PMID: 22109557]
• Patino, GA et al. Voltage-Gated Na+ Channel {beta}1B: A Secreted Cell Adhesion Molecule Involved in Human Epilepsy. J Neurosci 31: 14577-91. 2011-01-01 [PMID: 21994374]
• Liebau, MC et al. Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia. J Biol Chem. 2011-02-28 [PMID: 21357692]
• Gallazzini, M et al. High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization. Mol Biol Cell 22: 703-14. 2011-01-01 [PMID: 21209322]
• Herskowitz, JH et al. Rho Kinase II Phosphorylation of the Lipoprotein Receptor LR11/SORLA Alters Amyloid-{beta} Production. J Biol Chem 286: 6117-27. 2011-01-01 [PMID: 21147781]
• Zhao, A et al. Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform. J Immunol Methods 363: 221-32. 2011-01-01 [PMID: 20837020]
• Bjork, JK et al. miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis. Development;13719:3177-84. 2010-10-01 [PMID: 20724452]
• Hadler, KS et al. Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases?. BMC Res Notes. 1: 78. 2008-01-01 [PMID: 18771593]
• Boggio, R et al. Targeting SUMO E1 to ubiquitin ligases: a viral strategy to counteract sumoylation. J Biol Chem 282: 15376-82. 2007-01-01 [PMID: 17392274]
• Stuart A MacNeill. The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the B-subunit . Nucleic Acids Research 32: 3005-3016. 2004-01-01 [PMID: 15173383]
• Young, DF et al. Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells. J Virol 75 7: 3363 - 3370. 2001-01-01 [PMID: 11238862]
• Randall, RE et al. Purification of antibody-antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. Vaccine 12: 351-358. 1994-01-01 [PMID: 8178558]
• Hirst, K et al. The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal . EMBO J 13: 5410-5420. 1994-01-01 [PMID: 7957107]
• Jaffray, E et al. Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65 Mol. Cell Biol 15: 2166-2172. 1995-01-01 [PMID: 7891711]
• Arenzana-Seisdedos, F et al. Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol Cell Biol 15: 2689-2696. 1995-01-01 [PMID: 7739549]
• Rodriguez, MS et al. Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. Mol Cell Biol 15: 2413-2419. 1995-01-01 [PMID: 7739525]
• Hanke, T et al. Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding J. Virol Methods 149-156. 1995-01-01 [PMID: 7543487]
• Randall, RE et al. Two-tag purification of recombinant proteins for the construction of solid matrix-antibody antigen SMAA complexes as vaccines. Vaccine 11:1247-1252. 1993-01-01 [PMID: 7504859]
• Southern, JA et al. Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72: 1551-1557. 1991-01-01 [PMID: 1713260]
• Hanke, T et al. Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. J. Gen. Virol. 73: 653-660. 1992-01-01 [PMID: 1372038]

FAQs for Paramyxovirus SV5 Pk Antibody (NB100-62264). (Showing 1 - 1 of 1 FAQs).

1. I can't find any images or the antibody used in publications for this application, but the description says it works in this particular application. Could the company provide additional data?
   • This antibody has been validated in IHC on both frozen and paraffin-embedded tissues and is 100% guaranteed for this use. Validation came from customer feedback, so unfortunately, these images are not available. I apologize for the inconvenience. If you are concerned about images, you may want to consider NB600-381 or NB100-62264 as these two products both have over 20 publications.

Bioinformatics

• Uniprot:
  • P11207(Virus)