V5 Epitope Tag Antibody [Agarose]

• Reactivity: ET
• Applications: IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Agarose

V5 Epitope Tag Antibody [Agarose] Summary

• Immunogen: Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
• Application Notes: Immunoprecipitation: 15 - 25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract

Reactivity Notes

Parainfluenza virus 5 (PIV5) (Simian virus 5)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for V5 Epitope Tag Antibody [Agarose]

• GKPIPNPLLGLDST Epitope Tag
• Parainfluenza virus 5 V/P
• Paramyxovirus SV5
• V5 Epitope Tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V5 Epitope Tag Antibody (NB600-387)(18)

Publications using NB600-387

• Wu D, Wang S, Oliveira DV, et al The infantile myofibromatosis NOTCH3 L1519P mutation leads to hyperactivated ligand-independent Notch signaling and increased PDGFRB expression Dis Model Mech 2021-01-29 [PMID: 33509954] (IP)
  
  Details:
  Citation using the Agarose version of this antibody.
• Sticco MJ, Peña Palomino PA, Lukacsovich D, et al C1QL3 promotes cell-cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins FASEB J 2020-12-18 [PMID: 33337553]
  
  Details:
  Citation using the Agarose version of this antibody.
• Yu R, Liu T, Ning C et al. The phosphorylation status of Ser-637 in dynamin-related protein 1 (Drp1) does not determine Drp1 recruitment to mitochondria J Biol Chem. 2019-11-15 [PMID: 31533986] (IP, Human)
  
  Details:
  Citation used the Agarose format of this antibody.
• Liu T, Yu R, Jin SB et al. The mitochondrial elongation factors MIEF1 and MIEF2 exert partially distinct functions in mitochondrial dynamics Exp Cell Res 2013-07-20 [PMID: 23880462] (IP)
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Pempe EH, Xu Y, Gopalakrishnan S et al. Probing structural selectivity of synthetic heparin binding to Stabilin protein receptors J Biol Chem 2012-06-01 [PMID: 22547069]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• van der Aa LM, Jouneau L, Laplantine E et al. FinTRIMs, fish virus-inducible proteins with E3 ubiquitin ligase activity Dev Comp Immunol 2012-02-01 [PMID: 21907235]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Fuhs SR, Insel PA. Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization J Biol Chem 2011-04-01 [PMID: 21362625]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Ikeda M, Andoo A, Shimono M et al. The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function J Biol Chem 2011-02-01 [PMID: 21118806]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Bratschi MW, Burrowes DP, Kulaga A et al. Glycerol-3-phosphate acyltransferases gat1p and gat2p are microsomal phosphoproteins with differential contributions to polarized cell growth Eukaryot Cell 2009-08-01 [PMID: 19525420]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Park HA, Khanna S, Rink C et al. Glutathione disulfide induces neural cell death via a 12-lipoxygenase pathway Cell Death Differ 2009-08-01 [PMID: 19373248]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Asano N, Watanabe T, Kitani A et al. Notch1 signaling and regulatory T cell function J Immunol 2008-01-01 [PMID: 18292500]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Chigira Y, Oka T, Okajima T et al. Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domains Glycobiology 2008-04-01 [PMID: 18252777]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Nakamura Y, Weidinger G, Liang JO et al. The CCN family member Wisp3, mutant in progressive pseudorheumatoid dysplasia, modulates BMP and Wnt signaling J Clin Invest 2007-10-01 [PMID: 17823661]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Carattino, M et al. MEMBRANE TRANSPORT, STRUCTURE, FUNCTION, BIOGENESIS: The Epithelial Na+ Channel Is Inhibited by a Peptide Derived from Proteolytic Processing of Its alpha Subunit. J Biol Chem, 281: 18901-18907. 2006-01-01 [PMID: 16690613]
• Hughey, R et al. Distinct Pools of Epithelial Sodium Channels Are Expressed at the Plasma Membrane J Biol Chem 2004-01-01 [PMID: 15466477]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Zhang, Z et al. Selective incorporation of 5-hydroxytryptophan into protein in mammalian cells PNAS 2004-01-01 [PMID: 15187228] (Human)
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Hughey, R et al. Maturation of the Epithelial Na+ Channel Involves Proteolytic Processing of the alpha- gamma-Subuints Journal of Biological Chemistry 2003-01-01 [PMID: 12871941]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Miller, D et al. L166P Mutant DJ-1, Causative for Recessive Parkinsons Disease, Is Degraded through the Ubiqutin-Proteasome System Journal of Biological Chemistry 2003-01-01 [PMID: 12851414]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.