V5 Epitope Tag Antibody [Agarose Immobilized]

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Product Details

Summary
Reactivity NASpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Goat
Conjugate
Agarose Immobilized

V5 Epitope Tag Antibody [Agarose Immobilized] Summary

Immunogen
Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:10-1:500
Application Notes
Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.
Publications
Read Publications using
NB600-387 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for V5 Epitope Tag Antibody [Agarose Immobilized]

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V5 Epitope Tag Antibody (NB600-387)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


Filter By Application
IP
(1)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB600-387 Applications Species
Liu T, Yu R, Jin SB et al. The mitochondrial elongation factors MIEF1 and MIEF2 exert partially distinct functions in mitochondrial dynamics. Exp Cell Res 2013 Jul 20 [PMID: 23880462] (IP) IP
Pempe EH, Xu Y, Gopalakrishnan S et al. Probing structural selectivity of synthetic heparin binding to Stabilin protein receptors. J Biol Chem 2012 Jun [PMID: 22547069]
van der Aa LM, Jouneau L, Laplantine E et al. FinTRIMs, fish virus-inducible proteins with E3 ubiquitin ligase activity. Dev Comp Immunol 2012 Feb [PMID: 21907235]
Fuhs SR, Insel PA. Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization. J Biol Chem 2011 Apr [PMID: 21362625]
Ikeda M, Andoo A, Shimono M et al. The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function. J Biol Chem 2011 Feb [PMID: 21118806]
Bratschi MW, Burrowes DP, Kulaga A et al. Glycerol-3-phosphate acyltransferases gat1p and gat2p are microsomal phosphoproteins with differential contributions to polarized cell growth. Eukaryot Cell 2009 Aug [PMID: 19525420]
Park HA, Khanna S, Rink C et al. Glutathione disulfide induces neural cell death via a 12-lipoxygenase pathway. Cell Death Differ 2009 Aug [PMID: 19373248]
Asano N, Watanabe T, Kitani A et al. Notch1 signaling and regulatory T cell function. J Immunol 2008 Mar [PMID: 18292500]
Chigira Y, Oka T, Okajima T et al. Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domains. Glycobiology 2008 Apr [PMID: 18252777]
Nakamura Y, Weidinger G, Liang JO et al. The CCN family member Wisp3, mutant in progressive pseudorheumatoid dysplasia, modulates BMP and Wnt signaling. J Clin Invest 2007 Oct [PMID: 17823661]
Show All 15 Publications.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB600-380B
FITC Labeled NB600-385
HRP Labeled NB600-386

Additional V5 Epitope Tag Products

V5 Epitope Tag NB600-387

Research Areas for V5 Epitope Tag Antibody (NB600-387)

Find related products by research area.

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

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