| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47862-0.1mg | |||
| IHC-Prediluted | NBP2-48320 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56475SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47860-0.1mg | |||
| IHC-Prediluted | NBP2-48317 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24859SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27403 | |||
| BSA Free | NB100-56546SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2090SS | |||
![Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RENT1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RENT1-UPF1-hUPF1-Antibody-Western-Blot-NB100-368-img0004.jpg "Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RENT1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/RENT1-UPF1-hUPF1-Antibody-Western-Blot-NB100-368-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44613-img0009.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0010.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (364-5 + NCL/902).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Flow-Cytometry-NBP2-44613-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5 + NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44613-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Testicular Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902)](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5 + NCL/902) [NBP2-44613] - Human Uterus stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-+-NCL-902-Immunohistochemistry-Paraffin-NBP2-44613-img0006.jpg)
![Western Blot: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Western-Blot-NB100-558-img0016.jpg "Western Blot: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunohistochemistry-Paraffin: SETD1A Antibody [NB100-558] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSET1 used at 1:5,000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunohistochemistry-Paraffin-NB100-558-img0017.jpg)
![Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - Chromatin Immunoprecipitation: SETD1A Antibody [NB100-558] - ChIP-chip scatter plot of anti-hSET1 (NB100-558) enriched DNA binding sites versus input reference DNA. A. 10 mcg of NB100-558 was used to immunoprecipitate chromatin from K562 cells per Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-hSET1 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Chromatin-Immunoprecipitation-NB100-558-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SETD1A Antibody [NB100-558] - Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hSET1 antibody NB100-558 (lot NB100-558-6) used for IP at 6 ug per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-558-5) and another rabbit anti-hSET1 antibody. For blotting immunoprecipitated hSET1, the other antibody was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SETD1A-Antibody-Immunoprecipitation-NB100-558-img0015.jpg)
![Western Blot: LSD1 Antibody [NB100-56475] - Analysis of LSD1 in the 1) absence and 2) presence of immunizing peptide in human brain lysate using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NB100-56475-img0002.jpg "Western Blot: LSD1 Antibody [NB100-56475] - Analysis of LSD1 in the 1) absence and 2) presence of immunizing peptide in human brain lysate using this antibody. 5 ug/ml.")
![Western Blot: Nucleolin Antibody (NCL/902) [NBP2-44610] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Western-Blot-NBP2-44610-img0009.jpg "Western Blot: Nucleolin Antibody (NCL/902) [NBP2-44610] - A431 Cell Lysate using Nucleolin Monoclonal Antibody (NCL/902)")
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0016.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human colon stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0021.jpg)
![Flow Cytometry: Nucleolin Antibody (NCL/902) [NBP2-44610] - Flow Cytometry of Human Nucleolin Ag on Jurkat cells. Grey: Isotype Control; Green: FITC-labeled; Purple: APC-Labeled; Turquoise: AF700 Labeled Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Flow-Cytometry-NBP2-44610-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - HeLa cells stained with AF488 labeled Nucleolin Antibody (NCL/902) Green: AF488-labeled Ab. Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (NCL/902)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (NCL/902) [NBP2-44610] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (NCL/902) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunocytochemistry-Immunofluorescence-NBP2-44610-img0015.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Bladder carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Skin stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Human Uterus stained with Nucleolin Monoclonal Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0018.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/902) [NBP2-44610] - Formalin-fixed, paraffin-embedded human Uterus stained with Nucleolin Antibody (NCL/902).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-902-Immunohistochemistry-Paraffin-NBP2-44610-img0019.jpg)
![Western Blot: BTF Antibody [NBP2-24859] - Total protein from human A431 and U2Os cells and mouse 3T3 and Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-BTF (NBP2-24859) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume Chemiluminescent detection reagent (NBP2-61915)](http://images.novusbio.com/fullsize/BTF-Antibody-Western-Blot-NBP2-24859-img0004.jpg "Western Blot: BTF Antibody [NBP2-24859] - Total protein from human A431 and U2Os cells and mouse 3T3 and Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-BTF (NBP2-24859) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume Chemiluminescent detection reagent (NBP2-61915)")
![Immunocytochemistry/Immunofluorescence: BTF Antibody [NBP2-24859] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BTF at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24859-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BTF Antibody [NBP2-24859] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BTF at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24859-img0005.jpg)
![Immunohistochemistry-Paraffin: BTF Antibody [NBP2-24859] - analysis of human spleen probed with BTF antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/BTF-Antibody-Immunohistochemistry-Paraffin-NBP2-24859-img0002.jpg)
![Western Blot: BTF Antibody [NBP2-24859] - analysis of human BTF in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using BTF antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/BTF-Antibody-Western-Blot-NBP2-24859-img0001.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunocytochemistry-Immunofluorescence-NB100-56546-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunohistochemistry-Paraffin-NB100-56546-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) [NB100-56546] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Flow-Cytometry-NB100-56546-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0001.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0015.jpg "Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP1-33061] - Analysis of paraformaldehyde-fixed HeLa.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33061-img0003.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0018.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0007.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Sample (30 ug of whole cell lysate) NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0008.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0013.jpg)
![Western Blot: DDB1 Antibody [NBP1-33061] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0014.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Cal27 Xenograft , using DDB1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0005.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse prostate. DDB1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0011.jpg)
![Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Rat brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DDB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33061-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DDB1 Antibody [NBP1-33061] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody.](http://images.novusbio.com/fullsize/DDB1-Antibody-Western-Blot-NBP1-33061-img0012.jpg)
![Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/H2AX-Antibody-Western-Blot-NBP2-27428-img0004.jpg "Western Blot: H2AX Antibody [NBP2-27428] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: H2AX Antibody [NBP2-27428] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-H2AX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27428-img0007.jpg)
![Immunohistochemistry-Paraffin: H2AX Antibody [NBP2-27428] - Formalin-fixed, paraffin-embedded human spleen stained with Histone H2A.x antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunohistochemistry-Paraffin-NBP2-27428-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: H2AX Antibody [NBP2-27428] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-H2AX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/H2AX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27428-img0006.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NB21-2090-img0002.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.")
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NB21-2090-img0001.jpg)
![Simple Western: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Lane view shows a specific band for Histone H4 in 0.5 mg/ml of Hela Nuclear lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Simple-Western-NB21-2090-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0006.jpg "Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.")
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-42863-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NBP2-42863-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18 hrs) B. Control with 2.5 ug of pre-immune rabbit IgG C. Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 ug of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunoprecipitation-NBP2-42863-img0007.jpg)
![Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody (NBP2-42863) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42863_rabbit-polyclonal-histone-h4-ac-lys8-antibody-immunocytochemistry-immunofluorescence-261120251831543.jpg)
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0011.jpg "Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Rictor Antibody (1F3) [H00253260-M01] - Analysis of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunohistochemistry-Paraffin-H00253260-M01-img0008.jpg)
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.Lane 1: RICTOR transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0012.jpg)
![Immunoprecipitation: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunoprecipitation-H00253260-M01-img0009.jpg)
![Sandwich ELISA: Rictor Antibody (1F3) [H00253260-M01] - Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Sandwich-ELISA-H00253260-M01-img0010.jpg)