| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-1487SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1768SS | |||
| Azide and BSA Free | NBP2-80668 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-600SS | |||
| Azide and BSA Free | NBP2-80602 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54808SS | |||
| Azide and BSA Free | NBP2-80729 | |||
![Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NB100-1487-img0003.jpg "Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.")
![Simple Western: Rad50 Antibody [NB100-1487] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Rad50-Antibody-Simple-Western-NB100-1487-img0004.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0003.jpg "Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).")
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunocytochemistry-Immunofluorescence-NB100-1768-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunohistochemistry-Paraffin-NB100-1768-img0006.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0002.jpg)
![Western Blot: hnRNP F Antibody [NBP1-33607] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hnRNP-F-Antibody-Western-Blot-NBP1-33607-img0013.jpg "Western Blot: hnRNP F Antibody [NBP1-33607] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: hnRNP F Antibody [NBP1-33607] - Paraformaldehyde-fixed A431, using hnRNP F (NBP1-33607) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/hnRNP-F-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33607-img0003.jpg)
![Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - Mouse uterus. hnRNP F antibody [N1N3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/hnRNP-F-Antibody-Immunohistochemistry-Paraffin-NBP1-33607-img0006.jpg)
![Western Blot: hnRNP F Antibody [NBP1-33607] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hnRNP-F-Antibody-Western-Blot-NBP1-33607-img0011.jpg)
![Immunohistochemistry-Paraffin: hnRNP F Antibody [NBP1-33607] - A549 Xenograft , using hnRNP F antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/hnRNP-F-Antibody-Immunohistochemistry-Paraffin-NBP1-33607-img0004.jpg)
![Immunoprecipitation: hnRNP F Antibody [NBP1-33607] - Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 ug of hnRNP F antibody [N1N3]. Western blot analysis was performed using hnRNP F antibody [N1N3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/hnRNP-F-Antibody-Immunoprecipitation-NBP1-33607-img0008.jpg)
![Western Blot: hnRNP K Antibody [NBP2-24532] - analysis of hnRNPK in K562 cell lysate in the 1) absence and2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP2-24532-img0001.jpg "Western Blot: hnRNP K Antibody [NBP2-24532] - analysis of hnRNPK in K562 cell lysate in the 1) absence and2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532] - Formalin-fixed, paraffin-embedded human adrenal tissue probed with hnRNPK antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP2-24532-img0005.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532] - Formalin-fixed, paraffin-embedded human thymus probed with hnRNPK antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP2-24532-img0004.jpg)
![Simple Western: hnRNP K Antibody [NBP2-24532] - Simple Western lane view shows a specific band for hnRNP K in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Simple-Western-NBP2-24532-img0006.jpg)
![Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis in HeLa nuclear Extracts.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP2-24531-img0007.jpg "Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis in HeLa nuclear Extracts.")
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24531] - Formalin-fixed, paraffin-embedded human adrenal tissue probed with hnRNPK antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP2-24531-img0005.jpg)
![Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis of hnRNPK in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this experiment.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP2-24531-img0002.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24531] - Formalin-fixed, paraffin-embedded human thymus probed with hnRNPK antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP2-24531-img0004.jpg)
![Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1557-img0008.jpg "Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NB100-1557] - Sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-NB100-1557-img0007.jpg)
![p97/VCP Antibody [NB100-1557] - Detection of human and mouse VCP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody NB100-1557 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-NB100-1557-img0009.jpg)
![p97/VCP Antibody [NB100-1557] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1557 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1558. For blotting immunoprecipitated VCP, NB100-1557 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-NB100-1557-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunocytochemistry-Immunofluorescence-NBP2-43697-img0010.jpg "Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ug of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ug p21 Cip1 antibody [1032] 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [1032] diluted at 1:1000. [EasyBlot anti-mouse IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunoprecipitation-NBP2-43697-img0002.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunocytochemistry-immunofluorescence-26112025183418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-26112025194811.jpg)
![Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251846105.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.p21 Cip1 stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunohistochemistry-paraffin-2611202518315421.jpg)
![p21 Cip1 antibody [GT1032] (NBP2-43697) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT1032] (NBP2-43697) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-flow-cytometry-2611202518321914.jpg)
![Untreated (-) and treated (+) HCT 116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251836336.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0021.jpg "Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-31311] - Analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31311-img0008.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0019.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - DLD-1 xenograft, using Mad2L1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0009.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0012.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NBP1-31311-img0017.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0004.jpg "Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.")
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] NB100-600C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0006.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 in MCF-7 whole cell lysate using NB100-600. 2 minute ECL exposure.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0003.jpg)
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0005.jpg)
![Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Knockout-Validated-NBP2-54808-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: FANCD2 Antibody (1290D) [NBP2-54808] - Detection of Human FANCD2 by Simple Western using FANCD2 Antibody (1290D) in HeLa and K562 cell lysate.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Simple-Western-NBP2-54808-img0002.jpg)
![Immunohistochemistry: FANCD2 Antibody (1290D) [NBP2-54808] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Antibody (1290D) (Catalog # NBP2-54808) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Immunohistochemistry-NBP2-54808-img0004.jpg)
![Flow (Intracellular): FANCD2 Antibody (1290D) [NBP2-54808] - An intracellular stain was performed on HeLa Cells with FANCD2 Antibody (1290D) (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Flow-Intracellular-NBP2-54808-img0005.jpg)
![Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Total protein from HeLa and K562 cells (Molecular weight: 164.1 KDa) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.1 ug/ml FANCD2 Antibody (1290D) (catalog number NBP2-54808) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290D-Western-Blot-NBP2-54808-img0006.jpg)
![Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Analysis of XRCC6 expression in transfected 293T cell line by G22P1 monoclonal antibody (M01), clone 4C2-1A6.Lane 1: XRCC6 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-4C2-1A6-Western-Blot-H00002547-M01-img0017.jpg "Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Analysis of XRCC6 expression in transfected 293T cell line by G22P1 monoclonal antibody (M01), clone 4C2-1A6.Lane 1: XRCC6 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Analysis of monoclonal antibody to XRCC6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-4C2-1A6-Immunocytochemistry-Immunofluorescence-H00002547-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Analysis of monoclonal antibody to XRCC6 on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-4C2-1A6-Immunohistochemistry-Paraffin-H00002547-M01-img0014.jpg)
![Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - XRCC6 monoclonal antibody (M01), clone 4C2-1A6 Analysis of XRCC6 expression in C32.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-4C2-1A6-Western-Blot-H00002547-M01-img0016.jpg)
![Sandwich ELISA: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Detection limit for recombinant GST tagged XRCC6 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-4C2-1A6-Sandwich-ELISA-H00002547-M01-img0015.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0016.jpg "Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunocytochemistry-Immunofluorescence-H00003065-M14-img0009.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunohistochemistry-Paraffin-H00003065-M14-img0010.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0012.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0013.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0014.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0015.jpg)
![ELISA: HDAC1 Antibody (5C11) [H00003065-M14] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-ELISA-H00003065-M14-img0008.jpg)
![Proximity Ligation Assay: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of protein-protein interactions between NFKB1 and HDAC1. HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Proximity-Ligation-Assay-H00003065-M14-img0011.jpg)