| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06609SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50165-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37736-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54472-100ug | |||
![Western Blot: RPA70 Antibody [NB100-2204] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiRPA70 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA70-Antibody-Western-Blot-NB100-2204-img0015.jpg "Western Blot: RPA70 Antibody [NB100-2204] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiRPA70 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Human ovarian carcinoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No.used at a dilution of 1:1,000 (1 ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml (right). Detection: DAB](http://images.novusbio.com/fullsize/RPA70-Antibody-Immunohistochemistry-Paraffin-NB100-2204-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RPA70 Antibody [NB100-2204] - Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and (15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody NB100-2204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RPA70-Antibody-Western-Blot-NB100-2204-img0014.jpg)
![Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti- RPA70 Lot2 used at a dilution of 1:1,000 (1 ug/ml) (left) and Lot3 used at a dilution of1:5,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/RPA70-Antibody-Immunohistochemistry-NB100-2204-img0012.jpg)
![Immunoprecipitation: RPA70 Antibody [NB100-2204] - Detection of human RPA70 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPA70 antibody NB100-2204 (lot NB100-2204-3) used for IP at 6 ug per reaction. RPA70 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2204-2). For blotting immunoprecipitated RPA70, NB100-2204 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPA70-Antibody-Immunoprecipitation-NB100-2204-img0017.jpg)
![Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Fen1 antibody NB100-320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Western-Blot-NB100-320-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Fen1 antibody NB100-320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa cells (1 mg loaded), affinity purified rabbit antiFen1 antibody used for IP at 3 ug/mg lysate. Fen1 was also immunoprecipitated by rabbit anti-Fen1 antibody NB100-321, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunoprecipitation-NB100-320-img0007.jpg)
![Flap excision activity in proliferating and differentiated CAD cells. (A) Substrate for the flap endonuclease assay. The double-flap structure is optimal for Fen1 [27]; the target strand for that enzyme is labeled on its 5′ end the TAMRA fluor. The expected Fen1 product is shown. (B) Representative gel for the flap excision assay using whole-cell extracts from Prolif and Diff cells. Increasing amounts of extract were used for 1 pmol of substrate; Fen1 (51 nmol) was used as a positive control. The incubation was performed at 37 C for 30 min. (C) Quantification using Image J (version 2.0.0-rc-68/1.52i) (n = 3). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37628896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-320_rabbit-polyclonal-fen-1-antibody-western-blot-13220261219353.jpg)
![Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Western-Blot-NB100-2312-img0016.jpg "Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312] - Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXO1a antibody NB100-2312 (lot NB100-2312-3) used for IP at 6 ug per reaction. FOXO1a was also immunoprecipitated by a previous lot of this antibody (lot NB100-2312-2). For blotting immunoprecipitated FOXO1a, NB100-2312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunoprecipitation-NB100-2312-img0015.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].](http://images.novusbio.com/fullsize/NBS1-Antibody-Immunohistochemistry-Paraffin-NBP1-06609-img0003.jpg "Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].")
![Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.](http://images.novusbio.com/fullsize/NBS1-Antibody-Western-Blot-NBP1-06609-img0004.jpg)
![Immunohistochemistry: Nbs1 Antibody - BSA Free [NBP1-06609] - Immunohistochemical staining of MRE11, RAD50 & NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), & NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), & NBS1 (f) are shown (40× magnification) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30176843), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06609_rabbit-polyclonal-nbs1-antibody-31020241517139.jpg)
![Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Western-Blot-NB100-74502-img0004.jpg "Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0010.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunohistochemistry-Paraffin-NB100-74502-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05447-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Rat testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0005.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Human testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0003.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Mouse testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0004.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385445.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of NIH/3T3, using Phospho-ATR-S428 antibody at 1:400 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385627.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0029.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0024.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-NBP1-31294-img0004.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0012.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP1-31294-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31294-img0003.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0019.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0020.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0021.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunohistochemistry-Paraffin-NBP1-31294-img0022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242041272.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420134061.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.</br>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: HDAC1 stained by HDAC1 antibody (NBP1-31294) diluted at 1:500.</br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.</br>Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420131216.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC1 antibody (NBP1-31294) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242001098.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Sample (30 ug of whole cell lysate) <br /> A: zebrafish eye <br /> 10% SDS PAGE <br /> NBP1-31294 diluted at 1:1000 <br />](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115068.jpg)
![Immunohistochemistry: HDAC1 Antibody [NBP1-31294] - HDAC1 antibody detects Hdac1 protein on zebrafish by whole mount immunohistochemical analysis. <BR/>Sample: 2 days-post-fertilization zebrafish embryo. <BR/>HDAC1 antibody (NBP1-31294) dilution: 1:100.<BR/>](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-910202420115022.jpg)
![Western Blot: HDAC1 Antibody [NBP1-31294] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (NBP1-31294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31294_rabbit-polyclonal-hdac1-antibody-91020242021910.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) [NBP2-53247] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/1690).](http://images.novusbio.com/fullsize/CDX2-Antibody-CDX2-1690-Immunohistochemistry-Paraffin-NBP2-53247-img0002.jpg "Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) [NBP2-53247] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/1690).")
![Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) [NBP2-53247] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/1690).](http://images.novusbio.com/fullsize/CDX2-Antibody-CDX2-1690-Immunohistochemistry-Paraffin-NBP2-53247-img0001.jpg)
![Protein Array: CDX2 Antibody (CDX2/1690) [NBP2-53247] - Analysis of Protein Array containing >19,000 full-length human proteins using Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CDX2-Antibody-CDX2-1690-Protein-Array-NBP2-53247-img0003.jpg)
![Western Blot: Timeless Antibody [NB100-40853] - Detection of human and mouse Timeless by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody NB100-40853 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Timeless-Antibody-Western-Blot-NB100-40853-img0006.jpg "Western Blot: Timeless Antibody [NB100-40853] - Detection of human and mouse Timeless by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody NB100-40853 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: Timeless Antibody [NB100-40853] - Detection of human Timeless by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless (NB100-40853). Detection: DAB](http://images.novusbio.com/fullsize/Timeless-Antibody-Immunohistochemistry-NB100-40853-img0007.jpg)
![Western Blot: Timeless Antibody [NB100-40853] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Timeless antibody NB100-40853 used for IP at 6 ug per reaction. Timeless was also immunoprecipitated by rabbit anti-Timeless antibody NB100-40852. For blotting immunoprecipitated Timeless, NB100-40853 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Timeless-Antibody-Western-Blot-NB100-40853-img0003.jpg)
![Western Blot: Timeless Antibody [NB100-40853] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-Timeless antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescencewith an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Timeless-Antibody-Western-Blot-NB100-40853-img0005.jpg)
![Immunohistochemistry-Paraffin: Timeless Antibody [NB100-40853] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Timeless-Antibody-Immunohistochemistry-Paraffin-NB100-40853-img0004.jpg)
![Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Western-Blot-NB200-335-img0006.jpg "Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NB200-335-img0008.jpg)
![Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunoprecipitation-NB200-335-img0013.jpg)