| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88517-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76638SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Unpurified | NB100-185SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-2044V | |||
| FITC | NB21-2044F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2044SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1768SS | |||
| Azide and BSA Free | NBP2-80668 | |||
![Western Blot: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Western-Blot-NBP3-11038-img0001.jpg "Western Blot: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0008.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0003.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) - Azide and BSA Free [NBP3-11038] - Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-NCL-7014R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11038-img0007.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Analysis in human testis and liver tissues using NBP1-84416 antibody. Corresponding RBM14 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RBM14 Antibody [NBP1-84416] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84416-img0009.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0013.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0011.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0012.jpg)
![Immunohistochemistry-Paraffin: RBM14 Antibody [NBP1-84416] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RBM14-Antibody-Immunohistochemistry-Paraffin-NBP1-84416-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88517-img0008.jpg "Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0014.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0001.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0011.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0012.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0013.jpg)
![Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Western blotting showing TP53INP1 protein levels in NIH-H460 cells treated LNA-663. TUBULIN was used as protein loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-017-0080-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Western-Blot-NBP1-76638-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Western blotting showing TP53INP1 protein levels in NIH-H460 cells treated LNA-663. TUBULIN was used as protein loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-017-0080-x) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - p53 DINP1 Antibody [NBP1-76638] - Expression in human lung tissue lysate with p53DINP1 antibody at (A) 0.5 and (B) 1 u/mL.](http://images.novusbio.com/fullsize/p53-DINP1-Antibody-Western-Blot-NBP1-76638-img0001.jpg)
![Western Blot: p53 DINP1 Antibody - BSA Free [NBP1-76638] - miR-663 controls PIN-1-mediated apoptosis & p53 mitochondrial functiona Immunoblot showing BTG2 downmodulation 72 h after siRNA transfection. TUBULIN was used as protein loading control. Normalized BTG2 levels are expressed as fold decrease relative to cntrl treated cells. b Western blotting showing BTG2 modulation in NIH-H460 after the indicated treatments. Double transfection of anti-BTG2 siRNA together with LNA-663 restores BTG2 levels of control cells, counteracting LNA-mediated induction. PARP-1 cleavage is reduced to 60%. TUBULIN was used as protein loading control. Numbers indicate BTG2 levels fold change relative to cntrl treated cells, & cleaved PARP-1 levels fold decrease relative to LNA-663/cntrl siRNA-treated cells. The values were normalized to TUBULIN signal, as measured by densitometric analyses. c Subcellular BTG2 & PIN-1 localization in NIH-H460 cells treated with control LNA or LNA-663 was assessed by immunofluorescence staining. d Mitochondrial membrane potential of NIH-H460 cells upon miR-663 downmodulation was measured by JC-1 staining. An increase of the ratio between green & red signals is indicative of membrane depolarization typical of apoptotic cells. e Western blotting showing PIN-1 modulation in NIH-H460 subcellular fractions 24 h after LNA-663 treatment. Cytoplasmic accumulation & nuclear decrease of PIN-1 are expressed as fold changes relative to cntrl-treated cells. The histogram shows the ratio between cytoplasmic & nuclear signal. f Immunoblot of p53 in NIH-H460 subcellular fractions. Hsp90 & Bcl-2 were used as cytoplasmic & mitochondrial markers, respectively. Mitochondrial p53 modulation is expressed as fold increase relative to cntrl treated cells & normalized to Bcl-2 signal Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29352138), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-31020241616377.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunofluorescence of p53 DINP1 in human liver tissue with p53 DINP1 antibody at 5 ug/ml. Green: p53 DINP1 antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-2711202419353887.gif)
![Immunohistochemistry: p53 DINP1 Antibody - BSA Free [NBP1-76638] - Immunohistochemical staining of mouse liver using p53 DINP1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76638_rabbit-polyclonal-p53-dinp1-antibody-2711202420352418.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0003.jpg "Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Staining in canine blood cells from a verified customer review.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-OTI1F12-Immunocytochemistry-Immunofluorescence-NBP2-46125-img0005.jpg)
![Western Blot: Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of extracts (35ug) from 9 different cell lines by using CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Western-Blot-NBP2-46125-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD22.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-1F12-Immunofluorescence-NBP2-46125-img0002.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Western-Blot-NB100-692-img0003.jpg "Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin fixed paraffin embedded human tonsil stained with MGMT antibody.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0002.jpg)
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MGMT MT 3.1 (Cat. #NB 100-692) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0001.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - MMR protein expression & hMutS alpha activity decreases while hprt mutation rates & MNNG resistance increases in MGMT & MMR negative subclones after repeated exposure to MNNG.A. MMR protein expression, each fluorescent protein band was measured against a loading control (p62) in the same lane by Alpha lnnotech Fluorochem HD2, histograms produced by Prism GraphPad software, error bars indicate SD. U251 not represented in histogram because hMSH6 & hMSH2 expression is completely absent (Figure 1). Asterisks (*) denote statistically significant differences at P < 0.05 between each subclone protein expression & HeLa MR for the designated MMR protein. Statistical significance determined by student t-test using Prism GraphPad software. Each experiment was performed a minimum of 3 times. B. hMutS alpha binding activity of equal nuclear protein concentration from nuclear extracts of each cell line by EMSA using [32P]-69mer oligomers with either G:T or 06meG:T located in the center. C. hprt mutation rates in two sequentially isolated HeLa MNNGR clones as compared to HeLa MR. D. Classic colony survival of HeLa MNNG4 & U251R subclones indicates significant resistance to 0.2 µM MNNG as compared to HeLa MR & U251 parental cell lines. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24019948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-692_mouse-monoclonal-mgmt-antibody-mt-3-1-31020241612636.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0001.jpg "Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250")
![Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.](http://images.novusbio.com/fullsize/PARP2-Antibody-Western-Blot-NB100-185-img0002.jpg)
![Western Blot: PARP2 Antibody [NB100-185] - Activation of signaling pathways by dandelion root extract. Western blots of proteins involved in programmed cell death and cell survival and inflammation. The protein levels, including ULK1 (NBP2-24738), Bcl-2 related protein A1 (NBP1-76715), and PARP2 (NB100-185) corresponded to the mRNA levels obtained during the gene expression analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27564258/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb100-185_rabbit-polyclonal-parp2-antibody-unpurified-25520231539510.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0010.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0007.jpg)
![Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Flow-Cytometry-NBP2-67390-img0001.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0008.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0003.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0006.jpg)
![Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Western-Blot-NBP2-67326-img0008.jpg "Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0003.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BS](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0009.jpg)
![Flow Cytometry: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Flow-Cytometry-NBP2-67326-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0004.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0005.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0006.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0007.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0007.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Flow-Cytometry-NBP2-75640-img0001.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Rad51-Antibody-JM54-26-Immunohistochemistry-Paraffin-NBP2-75640-img0006.jpg)
![Western Blot: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of Rad51 on different lysates with Rabbit anti-Rad51 antibody at 1/1,000 dilution.Lane 1: Jurkat cell lysate (10 µg/Lane)Lane 2: NIH/3T3 cell lysate (10 µg/Lane)Lane 3: Mouse testis tissue lysate (20 µg/Lane)Predicted band size: 37 kDaObserved band size: 37 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-western-blot-2862024111350..jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of U-2 OS cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/100 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (ET1705-96) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111534..jpg)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (JM54-26) [NBP2-75640] - Analysis of NIH/3T3 cells labeling Rad51 with Rabbit anti-Rad51 antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Rad51 antibody (at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunocytochemistry-immunofluorescence-2862024111720..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] - Mouse spleen tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024111850..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Human tonsil tissue with Rabbit anti-Rad51 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-286202411203..jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody (JM54-26) [NBP2-75640] -Mouse testis tissue with Rabbit anti-Rad51 antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75640_rabbit-monoclonal-rad51-antibody-jm54-26-immunohistochemistry-paraffin-2862024112111..jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NB21-2044-img0006.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NB21-2044] - H4 K8ac antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2044-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NB21-2044] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-2044 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NB21-2044-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - Dot Blot analysis of H4 K8ac using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NB21-2044-img0004.jpg)
![Histone H4 [ac Lys8] Antibody [NB21-2044]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-NB21-2044-img0008.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0003.jpg "Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).")
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunocytochemistry-Immunofluorescence-NB100-1768-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunohistochemistry-Paraffin-NB100-1768-img0006.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0002.jpg)