| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61835-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1162V | |||
| FITC | NB21-1162F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1162SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20133-0.025mg | |||
![Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Western-Blot-NBP2-59217-img0001.jpg "Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - ChIP assays were performed using human HeLa cells, treated with TSA, the antibody against H3K18ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Chromatin-Immunoprecipitation-NBP2-59217-img0003.jpg)
![ELISA: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K18ac . The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-ELISA-NBP2-59217-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - HeLa cells were stained with the antibody against H3K18ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K18ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18-Dimethyl-Arg17]-Antibody-Immunofluorescence-NBP2-59217-img0004.jpg)
![Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Western blot analysis of lysates from wild type (WT) and SMARCC1 knockout (KO) 293T cells using [KO Validated] SMARCC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16367_rabbit-smarcc1-mab-1r7i6-522025125485.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Western blot analysis of lysates from wild type (WT) and SMARCC1 knockout (KO) 293T cells using [KO Validated] SMARCC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Western blot analysis of extracts of various cell lines, using SMARCC1 Rabbit mAb (NBP3-16367) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-1R7I6-Western-Blot-NBP3-16367-img0002.jpg)
![Chromatin Immunoprecipitation: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Chromatin immunoprecipitation analysis of extracts of MCF7 beta-estradiol cells, using SMARCC1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16367_rabbit-smarcc1-mab-1r7i6-52202511365149.jpg)
![Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Immunofluorescence analysis of NIH-3T3 cells using SMARCC1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16367_rabbit-smarcc1-mab-1r7i6-52202511365164.jpg)
![Western Blot: SmarcAL1 Antibody [NBP2-20414] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (NBP2-20414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20414_rabbit-polyclonal-smarcal1-antibody-910202420104425.jpg "Western Blot: SmarcAL1 Antibody [NBP2-20414] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (NBP2-20414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![SmarcAL1 antibody immunoprecipitates SMARCAL1 protein in IP experiments.IP samples: A549 whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of SMARCAL1 protein by 4 ug SmarcAL1 antibody (NBP2-20414)5 % SDS-PAGEThe immunoprecipitated SMARCAL1 protein was detected by SmarcAL1 antibody (NBP2-20414) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-20414_rabbit-polyclonal-smarcal1-antibody-immunoprecipitation-2611202518354319.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Western-Blot-NBP2-59294-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Immunofluorescence-NBP2-59294-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K9me3 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 3 and 9 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Chromatin-Immunoprecipitation-NBP2-59294-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Cross reactivity of the antibody directed against H3K9me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K9me2. The wells were coated with peptides containing the unmodified H3K9 as well as the mono-, di- and trimethylated H3K9 and the dimethylated H3K4 and H3K27. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-ELISA-NBP2-59294-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Western-Blot-NBP2-59295-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Immunofluorescence-NBP2-59295-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me3 and optimized PCR primer sets for qPCR. ChIP was performed with the AutoHistone ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Chromatin-Immunoprecipitation-NBP2-59295-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Cross reactivity of the antibody directed against H3K27me3. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K27me3. The wells were coated with peptides containing the unmodified H3K27 region as well as the mono-, di- and trimethylated H3K27 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-ELISA-NBP2-59295-img0002.jpg)
![Western Blot: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K36me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Western-Blot-NBP2-59211-img0001.jpg "Western Blot: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K36me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Human osteosarcoma (U2OS) cells were stained with the antibody against H3K36me1 and with DAPI. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H3K36me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 568 or with DAPI (right), which specifically labels DNA. Figure B, C, D and E: staining of the cells with the H3K36me1 antibody after incubation of the antibody with 2 ng/ul blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K36, respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Immunofluorescence-NBP2-59211-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - ChIP assays were performed using human U2OS cells, the antibody against H3K36me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells and stringent washing conditions. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the constitutively expressed GAPDH gene and for the inactive gene MYOD. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59211-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36me1, crude serum and Flow Through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:46,000.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-ELISA-NBP2-59211-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - ChIP assays were performed using human HeLa cells treated with colcemid, the antibody against H3K27me3S28p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active gene GAPDH as a negative control, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3S28p is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27-p-Ser28]-Antibody-Chromatin-Immunoprecipitation-NBP2-59212-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Western blot was performed on histone extracts (15 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 2) using the antibody against H3K27me3S28p diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0005.jpg)
![ELISA: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27me3S28p. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-ELISA-NBP2-59212-img0001.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K27me3S28p with peptides containing other modifications of histone H3 and H4 and the unmodified H3K27S28 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:2,000. The figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Dot-Blot-NBP2-59212-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me2 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59194-img0003.jpg "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me2 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![Western Blot: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K79me2 diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-Western-Blot-NBP2-59194-img0001.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - Analysis was performed to test the cross reactivity of the Diagenode antibody against H3K79me2 with peptides containing other modifications and unmodified sequences of histone H3. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure 4 shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-Dot-Blot-NBP2-59194-img0005.jpg)
![ELISA: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K79me2 . The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:6,600.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-ELISA-NBP2-59194-img0002.jpg)
![Western Blot: AEBP2 Antibody (3E3C10) [NBP2-61835] - Analysis using AEBP2 mAb against HEK293 (1) and AEBP2 (AA: 358-495)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AEBP2-Antibody-3E3C10-Western-Blot-NBP2-61835-img0004.jpg "Western Blot: AEBP2 Antibody (3E3C10) [NBP2-61835] - Analysis using AEBP2 mAb against HEK293 (1) and AEBP2 (AA: 358-495)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: AEBP2 Antibody (3E3C10) [NBP2-61835] - Analysis of Hela cells using AEBP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/AEBP2-Antibody-3E3C10-Immunocytochemistry-Immunofluorescence-NBP2-61835-img0002.jpg)
![Western Blot: AEBP2 Antibody (3E3C10) [NBP2-61835] - Analysis using AEBP2 mAb against human AEBP2 (AA: 358-495) recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/AEBP2-Antibody-3E3C10-Western-Blot-NBP2-61835-img0003.jpg)
![Western Blot: AEBP2 Antibody (3E3C10) [NBP2-61835] - Analysis using AEBP2 mouse mAb against COS7 (1), HepG2 (2), and SK-MES-1 (3) cell lysate.](http://images.novusbio.com/fullsize/AEBP2-Antibody-3E3C10-Western-Blot-NBP2-61835-img0005.jpg)
![ELISA: AEBP2 Antibody (3E3C10) [NBP2-61835] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/AEBP2-Antibody-3E3C10-ELISA-NBP2-61835-img0001.jpg)
![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0005.jpg "Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of various cell lines, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Confocal imaging of Hep G2 cells using SMARCA5/SNF2H Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16216_rabbit-smarca5-snf2h-mab-6h8i7-5220251242527.jpg)
![Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Confocal imaging of C6 cells using SMARCA5/SNF2H Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16216_rabbit-smarca5-snf2h-mab-6h8i7-52202511365146.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys23] Antibody [NB21-1162] - Histone H3 [Dimethyl Lys23] antibody was tested at 1:25 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys23]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1162-img0004.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys23] Antibody [NB21-1162] - Histone H3 [Dimethyl Lys23] antibody was tested at 1:25 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.")
![Flow Cytometry: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C223 (NBP3-20133, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023924296.png "Flow Cytometry: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C223 (NBP3-20133, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C223 (NBP3-20133) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023924242.png)
![Immunocytochemistry/Immunofluorescence: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C223 (NBP3-20133) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20133, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023964611.png)