| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26007 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25985 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25982 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25998 | |||
![Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Immunocytochemistry-Immunofluorescence-H00008467-M02-img0003.jpg "Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Immunohistochemistry-Paraffin-H00008467-M02-img0005.jpg)
![Sandwich ELISA: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Detection limit for recombinant GST tagged SMARCA5 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Sandwich-ELISA-H00008467-M02-img0006.jpg)
![Western Blot: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Western blot analysis of p62/SQSTM1 on different lysates with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/500 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: RAW264.7 cell lysate (10 ug/Lane) Lane 3: PANC-1 cell lysate (10 ug/Lane) Lane 4: Mouse liver tissue lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32702) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-278202415295572.jpg "Western Blot: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Western blot analysis of p62/SQSTM1 on different lysates with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/500 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: RAW264.7 cell lysate (10 ug/Lane) Lane 3: PANC-1 cell lysate (10 ug/Lane) Lane 4: Mouse liver tissue lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32702) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32702) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-298202414161843.jpg)
![Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Immunocytochemistry analysis of A549 cells labeling p62/SQSTM1 with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-29202410484897.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25989_rabbit-histone-h3-mab-rm154-azide-and-bsa-free-22620242311080.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - RM154 specifically reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25989_rabbit-histone-h3-mab-rm154-azide-and-bsa-free-22620242311036.png)
![Western Blot: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP3-25989] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM154 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa.](http://images.novusbio.com/fullsize/nbp3-25989_rabbit-histone-h3-mab-rm154-azide-and-bsa-free-2462024132641.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25985_rabbit-histone-h3-mab-rm135-azide-and-bsa-free-226202422321235.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM135 at 0.025 ug/mL, showed a band of histone H3 dimethylated at Lysine 4 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25985_rabbit-histone-h3-mab-rm135-azide-and-bsa-free-22620242311042.png)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP3-25985] - RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp3-25985_rabbit-histone-h3-mab-rm135-azide-and-bsa-free-24620241332425.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26000_rabbit-histone-h3-mab-rm149-azide-and-bsa-free-2262024231101.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - RM149 specifically reacts to Histone H3 acetylated at Lysine 4. No cross reactivity with non-modified Lysine 4, acetylated Lysine 9, Lysine 14, Lysine 18, Lysine 23, Lysine 27, Lysine 36, Lysine 56, lysine 79, or Lysine 122 in histone H3.](http://images.novusbio.com/fullsize/nbp3-26000_rabbit-histone-h3-mab-rm149-azide-and-bsa-free-2462024132620.png)
![Western Blot: Histone H3 [ac Lys4] Antibody (RM149) - Azide and BSA Free [NBP3-26000] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM149 at 0.5 ug/mL, showed a band of Histone H3 acetylated at Lysine 4 in treated HeLa.](http://images.novusbio.com/fullsize/nbp3-26000_rabbit-histone-h3-mab-rm149-azide-and-bsa-free-22620242311010.png)
![Western Blot: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using NBP2-61537, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Western-Blot-NBP2-61537-img0007.jpg "Western Blot: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using NBP2-61537, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa. Observed molecular weight is ~16 kDa.")
![Immunohistochemistry: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Immunohistochemistry staining of HepG2 cells using NBP2-61537.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Immunohistochemistry-NBP2-61537-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP2-61537] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61537_rabbit-monoclonal-histone-h3-ac-lys56-antibody-rm179-immunocytochemistry-immunofluorescence-18920252338178.png)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61540 at 0.025 ug/ml, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-RM135-Western-Blot-NBP2-61540-img0004.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody (RM135) [NBP2-61540] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61540 at 0.025 ug/ml, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP2-61540] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61540_rabbit-monoclonal-histone-h3-dimethyl-lys4-antibody-rm135-immunocytochemistry-immunofluorescence-18920252339513.png)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys4] Antibody (RM135) - Azide and BSA Free [NBP2-61540] - RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp2-61540_rabbit-monoclonal-histone-h3-dimethyl-lys4-antibody-rm135-multiplex-immunoassay-18920252343566.png)
![Western Blot: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using NBP2-61532. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys14-Antibody-RM130-Western-Blot-NBP2-61532-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using NBP2-61532. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP2-61532] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61532_rabbit-monoclonal-histone-h3-ac-lys14-antibody-rm130-immunocytochemistry-immunofluorescence-18920252343561.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP2-61532] - ChIP performed on HeLa cells using RM130, 5ug. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61532_rabbit-monoclonal-histone-h3-ac-lys14-antibody-rm130-chromatin-immunoprecipitation-189202523485313.png)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Western-Blot-NBP2-61534-img0007.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP2-61534] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61534_rabbit-monoclonal-histone-h3-dimethyl-lys9-antibody-rm151-immunocytochemistry-immunofluorescence-18920252352116.png)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP2-61534] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61534_rabbit-monoclonal-histone-h3-dimethyl-lys9-antibody-rm151-chromatin-immunoprecipitation-189202523474821.png)
![Western Blot: BAF180/PB1 Antibody [NBP1-32025] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE PB1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Western-Blot-NBP1-32025-img0003.jpg "Western Blot: BAF180/PB1 Antibody [NBP1-32025] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE PB1 antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody [NBP1-32025] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32025-img0002.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NBP1-32025] - Paraffin-embedded mouse duodenum. PB1 antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunohistochemistry-Paraffin-NBP1-32025-img0004.jpg)
![Western Blot: SMARCA5/SNF2H Antibody [NBP3-12952] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:10000.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NBP3-12952-img0001.jpg "Western Blot: SMARCA5/SNF2H Antibody [NBP3-12952] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:10000.")
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP3-12952] - SMARCA5/SNF2H antibody detects SMARCA5/SNF2H protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunohistochemistry-Paraffin-NBP3-12952-img0002.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Western-Blot-NBP2-59288-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Immunofluorescence-NBP2-59288-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4me2 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-Chromatin-Immunoprecipitation-NBP2-59288-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys4] Antibody (C.15200151) [NBP2-59288] - Cross reactivity of the antibody directed against H3K4me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K4me2. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the dimethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys4-Antibody-C-15200151-ELISA-NBP2-59288-img0002.jpg)