| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85032-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26019 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26001 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84063-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30712-25ul | |||
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-26820241525100.jpg "Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Dot Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (negative) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (positive) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Lane 7: Acetyl-Histone H3 (Lys27) (negative) Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 50 seconds.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-28820241459250.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-2920241192347.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-3920248453547.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-24620241332472.png "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.")
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - RM151 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-22620242311096.png)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM151 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-22620242232120.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-2462024124593.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2462024133242.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2462024132628.png)
![Western Blot: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with Sodium Butyrate (+), using RM156 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2262024231100.png)
![Sandwich ELISA: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Sandwich ELISA against acetylated histone H3 at Lys 79 using Clone RM156 (5 ug/mL) as the capture antibody and another biotinylated Histone H3 (1 ug/mL) as the detection antibody.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-22620242311033.png)
![Multiplex Immunoassay: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-22620242311057.png "Multiplex Immunoassay: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-24620241245921.png)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Immunohistochemistry staining of HepG2 cells using Clone RM179.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-2462024132630.png)
![Western Blot: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using RM179 at 1 ug/mL, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-22620242311024.png)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-22620242232121.png "Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.")
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM150 at 0. 5 ug/mL, showed a band of histone H3 monomethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-2462024132634.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - Immunocytochemistry of HeLa cells, using Clone RM150 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-2462024124594.png)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - ChIP performed on HeLa cells using Clone RM150 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-chromatin-immunoprecipitation-2462024225540..png)
![Immunocytochemistry/Immunofluorescence: ZMIZ2 Antibody [NBP1-85032] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ZMIZ2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85032-img0004.jpg "Immunocytochemistry/Immunofluorescence: ZMIZ2 Antibody [NBP1-85032] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: ZMIZ2 Antibody [NBP1-85032] - Staining of human testis shows strong nuclear positivity in Leydig cells and cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ZMIZ2-Antibody-Immunohistochemistry-Paraffin-NBP1-85032-img0008.jpg)
![Immunohistochemistry-Paraffin: ZMIZ2 Antibody [NBP1-85032] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZMIZ2-Antibody-Immunohistochemistry-Paraffin-NBP1-85032-img0005.jpg)
![Immunohistochemistry-Paraffin: ZMIZ2 Antibody [NBP1-85032] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ZMIZ2-Antibody-Immunohistochemistry-Paraffin-NBP1-85032-img0006.jpg)
![Immunohistochemistry-Paraffin: ZMIZ2 Antibody [NBP1-85032] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ZMIZ2-Antibody-Immunohistochemistry-Paraffin-NBP1-85032-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) - Azide and BSA Free [NBP2-61546] - Immunocytochemistry of HeLa cells using Clone RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-immunocytochemistry-immunofluorescence-18920252353230.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) - Azide and BSA Free [NBP2-61546] - Immunocytochemistry of HeLa cells using Clone RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Histone H3 [p Thr3] Antibody (RM159) - Azide and BSA Free [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP3-26019 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-immunocytochemistry-immunofluorescence-189202523485321.png)
![Immunohistochemistry-Paraffin: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using NBP2-61546 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-131020231935490.png)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Western-Blot-NBP2-61535-img0007.jpg "Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP2-61535] - Immunocytochemistry of HeLa cells, using Clone RM150 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61535_rabbit-monoclonal-histone-h3-monomethyl-lys9-antibody-rm150-immunocytochemistry-immunofluorescence-189202523444720.png)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP2-61535] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp2-61535_rabbit-monoclonal-histone-h3-monomethyl-lys9-antibody-rm150-multiplex-immunoassay-189202523474823.png)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP2-61535] - ChIP performed on HeLa cells using Clone RM150 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61535_rabbit-monoclonal-histone-h3-monomethyl-lys9-antibody-rm150-chromatin-immunoprecipitation-189202523394315.png)
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and leydig cells.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0011.jpg "Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and leydig cells.")
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Cerebellum shows moderate nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0008.jpg)
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0009.jpg)
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Skin shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0010.jpg)
![Western Blot: AEBP2 Antibody [NBP2-30712] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/AEBP2-Antibody-Western-Blot-NBP2-30712-img0005.jpg "Western Blot: AEBP2 Antibody [NBP2-30712] - Analysis in human cell line BEWO.")
![Immunocytochemistry/Immunofluorescence: AEBP2 Antibody [NBP2-30712] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.](http://images.novusbio.com/fullsize/AEBP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30712-img0004.jpg)
![Immunohistochemistry: AEBP2 Antibody [NBP2-30712] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/AEBP2-Antibody-Immunohistochemistry-NBP2-30712-img0003.jpg)
![Western Blot: AEBP2 Antibody [NBP2-30712] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/AEBP2-Antibody-Western-Blot-NBP2-30712-img0002.jpg)
![Western Blot: WDR5 Antibody [NBP1-30070] - Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody NBP1-30070 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/WDR5-Antibody-Western-Blot-NBP1-30070-img0003.jpg "Western Blot: WDR5 Antibody [NBP1-30070] - Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody NBP1-30070 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- WDR5 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/WDR5-Antibody-Immunohistochemistry-NBP1-30070-img0002.jpg)
![Western Blot: WDR5 Antibody [NBP1-30070] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. WDR5 was successfully IPed by NBP1-30063.](http://images.novusbio.com/fullsize/WDR5-Antibody-Western-Blot-NBP1-30070-img0001.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-49955] - Detection of Human Sequestosome-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize other epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49955-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49955] - Detection of Human Sequestosome-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize other epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49955] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-NBP1-49955-img0003.jpg)