| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52721SS | |||
| Azide and BSA Free | NBP2-80824 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05950SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-111SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25154SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-107SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71309 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43999 | |||
| BSA Free | NBP2-59780-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-956-0.025ml | |||
![Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Analysis of 20 ug of HeLa cell lysate and 5 ul of Molecular Weight Protein Ladder per well.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Western-Blot-NB120-19294-img0005.jpg "Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Analysis of 20 ug of HeLa cell lysate and 5 ul of Molecular Weight Protein Ladder per well.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin D (CathD) expression and localization in primary neurons. Colocalization of transfected CathD with LAMP-1/CD107a. CathD transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathD. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NB120-19294-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Lamp1 (red) in HeLa cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NB120-19294-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin B (CathB) expression and localization in primary neurons. Colocalization of transfected CathB with LAMP-1/CD107a. CathB transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathB. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NB120-19294-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - EsxG-EsxH alters phagosomal GAL3, ubiquitin, & LAMP1. (A, C, & E) IF images of GAL3 (A), ubiquitin (FK2 antibody) (C), & LAMP1 (E) in BMDMs that were infected with DsRed-expressing H37Rv (WT) or the delta esxH mutant for 3 h. Images are maximum-intensity projections. Scale bar, 10 μm. Boxed areas in the merged image are shown in higher magnification in the rightmost panel. Mtb, M. tuberculosis. (B, D, & F) Automated image analysis was used to quantify the MFI of GAL3 (B), ubiquitin (D), & LAMP1 (F) colocalized with individual bacilli from 5 fields of a 12-mm coverslip. Data are means ± SEM from one representative experiment from three (A, B, E, & F) or two (C & D) independent experiments. ****, P ≤ 0.0001, Student's t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30482832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19294_rabbit-polyclonal-lamp-1-cd107a-antibody-310202415363712.jpg)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Characterization of selected mutant protein glycosylation status or localization.(A) Two independent, representative experiments to analyze the glycosylation status of GFP-NPC1 wild type & GFP-NPC1 P251C/L929C, as determined by immunoblot of indicated samples with anti-GFP antibodies after 6% SDS PAGE; samples were incubated at 70°C in SDS PAGE sample buffer for 10 min prior to loading. Molecular weight markers are shown at left in kilodaltons here & in all subsequent gels shown. (B), Localization of NPC1 P251/L929C/P249K/P259K in NPC1−/− HeLa cells as in Figure 1C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19294_rabbit-polyclonal-lamp-1-cd107a-antibody-310202415382428.jpg)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - NPC1 delta loop mutant cannot rescue cholesterol export from lysosomes.(A) Cholesterol-cross-linked peptides (Hulce et al., 2013) are highlighted in red for two orientations of the crystal structure of N-terminal domain- & first transmembrane domain-deleted NPC1 (PDBID: 5u74). The disordered cytoplasmic loop residues 800–814 are shown as a blue dotted line. (B) Confocal immunofluorescence microscopy analysis of the localization of mouse NPC1-delta 807–811, NPC1-807-811Ala & LAMP1 proteins in HeLa cells (bar, 20 μm). White boxes in images indicate regions of cells enlarged in the insets shown at the lower right of each image. (C) Confocal immunofluorescence microscopy of cholesterol accumulation rescue. NPC1−/− HeLa cells were transfected with GFP-tagged mouse NPC1-delta 807–811 or mouse NPC1-807-811Ala plasmids for 48 h & assayed for cholesterol accumulation rescue as in Figure 1 (bar, 20 μm). (D) Quantitation of cholesterol accumulation rescue using flow cytometry. GFP-positive cells with similar expression levels were analyzed: 2480 NPC1; 427 NPC1-delta 807–811; 764 NPC1-807-811Ala; LAMP1 expressing control, 1753 cells counted. Shown are the normalized data from mean fluorescence intensity flow cytometry values. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19294_rabbit-polyclonal-lamp-1-cd107a-antibody-310202416205170.jpg)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin D (CathD) & B (CathB) expression & localization in primary neurons. A. Western blot analyses of CathD & CathB protein levels after transfection. Primary cortical neurons were transfected with 23QHtt, 23QHtt plus CathD, 23QHtt plus CathB, 145QmHtt, 145QmHtt plus CathD & 145QmHtt plus CathB constructs. Western blot analyses were performed with anti-CathD & anti-CathB antibodies. beta -actin western blots were used as loading controls. Relative expression levels were quantified by band intensity. Positions of molecular weight markers were indicated. Quantification of the western blots are shown in the bar graphs. *p < 0.05 compared to without cathepsin transfection. B. Analyses of CathD & CathB enzymatic activities after transfection. Primary cortical neurons were transfected with CathD, CathB & as described in A. CathD & CathB activities were assayed by CathD or CathB activity assay kit. *p < 0.05 compared to without cathepsin transfection. C & D. Colocalization of transfected CathD (C) & CathB (D) with LAMP-1. CathD & CathB transfected neurons were examined by co-immunostaining of LAMP-1 & CathD or CathB. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins & LAMP-1. Scale bar = 10 micron. Image collected & cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19294_rabbit-polyclonal-lamp-1-cd107a-antibody-31020241621824.jpg)
![Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Effects on autophagic activity & dendritic length of neurons exposed to supernatant from HIV-1-infected microglia in combination with morphine. (A) Representative images of neurons with the indicated treatments. Sup, supernatant from uninfected [HIV(-)] & HIV-1-infected [HIV(+)] microglia. Cells were immunolabeled with antibodies to the autophagic activity marker p62/SQSTM1 (red) & the neuronal cell-type-specific marker MAP2 (green). DAPI (blue) staining indicates cell nuclei. (B) Quantification of p62/SQSTM1 immunoreactivity from (A). Data are presented as the percentage of control cells which was set at 100; F(5,24) = 5.882, p = 0.0011; ∗p < 0.05 when compared to HIV(+)Sup + morphine treatment. (C) Western blotting analysis of p62/SQSTM1 & LAMP1 expression levels for the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Measurement of dendrite length from (A). F(5,24) = 26.15, p = < 0.0001; Φp < 0.05 when compared to morphine; Ψp < 0.05 when compared to HIV(-)Sup; Ωp < 0.05 when compared to HIV(-)Sup + morphine; #p < 0.05 when compared to HIV(+)Sup; & ∗p < 0.05 when compared to HIV(+)Sup + morphine treatment. Error bars show the SEM for five randomly selected fields totaling at least 100 cells from each group. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19294_rabbit-polyclonal-lamp-1-cd107a-antibody-31020241621816.jpg)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - NPC1 Disulfide bond-locked MLD & CTD fails to rescue cholesterol export from lysosomes.(A) Partial NPC1 structure; inset, close-up view of the MLD/CTD interface. The amino acid residues mutated to cysteines for disulfide bond formation are shown & highlighted in red. (B) Confocal immunofluorescence microscopy analysis of mouse NPC1-A521C/K1013C & LAMP1 proteins in HeLa cells (bar, 20 μm). White boxes in images indicate regions of cells enlarged in the insets shown at the lower right of each image. (C) Confocal immunofluorescence microscopy of cholesterol accumulation rescue for NPC1-A521C or mouse NPC1-A521C/K1013C. (D) Flow cytometry of the rescue experiment analyzed in (C). GFP-positive cells with similar expression levels were analyzed: 17746 NPC1-/- cells; 1315 NPC1 wild type; 1137 NPC1-A521C/K1013C cells; 837 NPC1-A521C cells; cell numbers were normalized for comparison.Extracted ion chromatograms from LC-MS analysis of proteolyzed A521C/K1013C NPC1.Protein was carbamidomethylated in the presence or absence of reducing agent prior to deglycosylation & proteolysis. In both samples, blue traces represent m/z = 596.2818 (corresponding to the NPC1 peptide APCSLNDTSLL carbamidomethylated at the engineered cysteine A521C & deamidated at the N524 glycosylation site). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19294_rabbit-polyclonal-lamp-1-cd107a-antibody-31020241621838.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - ICC usage of NBP2-52721. Image submittied via verified customer review.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-52721-img0005.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - ICC usage of NBP2-52721. Image submittied via verified customer review.")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all the ductal and tubular epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-52721-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LAMP (5E7) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-52721-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - MCF-7 human breast cancer cell line. Secondary antibody goat anti-mouse IgG Fc Dy680 (NBP1-72880). Image from verified customer review.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-52721-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all cells, especially the ductal and tubular epithelial cells. Few cells from the glomeruli showed nuclear signal also.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-52721-img0002.jpg)
![Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0006.jpg "Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.")
![Immunocytochemistry/Immunofluorescence: PLTP Antibody [NB400-106] - PLTP antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunocytochemistry-Immunofluorescence-NB400-106-img0002.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression and activity in RA and OA joints and 3OH-myristate levels in synovial fluid of RA and OA patients. Immunohistological analysis of PLTP expression in synovial tissue from 5 RA and 6 OA patients stained for PLTP and macrophages (CD68+ cells); All RA synovial tissue showed positive stainings while only 2 out 6 OA tissues were positive for PLTP staining. Representative stainings are shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0008.jpg)
![Western Blot: PLTP Antibody [NB400-106] - WB analysis of PLTP in human brain lysate.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0001.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP antibody expression in RA synovial tissues. (A) Immuno-histological analyses used RA patients synovial tissue sections (n=5). They were stained for PLTP or macrophages (CD68+ cells). Blue arrows indicate macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with CD68+ staining (black arrow; non-exhaustive) and morphological features. (B, C) Double staining displays localization (n = 3) of PLTP with macrophages (CD68+) in B or RA-FLS (alpha-SMA+) in C. Overlay is shown to visualize co-localization of PLTP. All magnification and fluorescence analysis at 20x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0003.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - Recombinant PLTP antibody induced cytokine production and FLS proliferation, independent of its lipid transfer ability. (A) RA-FLS were stimulated for 48 hours with PLTP or heat inactivated-PLTP and proliferation was evaluated during the final day of stimulation using thymidine incorporation. (B) Blockade of PLTP decreased the effect of rhPLTP on FLS proliferation. (C) PLTP impact on RA-FLS cytokine production. (D) OA-FLS were treated with either native PLTP or heated-PLTP and analyzed for cytokine production and proliferation. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0005.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106] - PLTP was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human PLTP polyclonal Antibody (Catalog # NB400-106) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in decidual cells.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-Paraffin-NB400-106-img0007.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression in RA synovial tissues.For immuno-histological analysis, (A) synovial tissue sections from RA patients (n = 5) were stained for PLTP or macrophages (CD68+ cells). Representative images obtained for immunohistological staining are shown. Blue arrows show macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with morphological features & CD68+ staining (black arrow; non-exhaustive). (B, C) Double staining was performed to visualize localization (n = 3) of PLTP with macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C). Fluorescence was analyzed at 20x magnification. Overlay is shown to visualize co-localization of PLTP in macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C) or PLTP expression in infiltrate. Original magnification: 20x. Separate images can be found in S1 Fig. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0193815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-106_rabbit-polyclonal-pltp-antibody-310202416171467.jpg)
![Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Western-Blot-NBP2-25183-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - HeLa cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-25183-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - IHC staining of LAMP-1 [H4A3] catalog number NBP2-25183 in mouse liver. Cytoplasmic and membrane staining was observed in tubule cells and glomeruli.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunohistochemistry-Paraffin-NBP2-25183-img0014.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0017.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human kidney.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunohistochemistry-Paraffin-NBP2-25183-img0006.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A549 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0009.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0004.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on THP-1 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0008.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0010.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) APC.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0011.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor (R) 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-NBP2-25183-img0012.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Analysis of PHA-activated PBMC with anti-CD107a (H4A3) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0013.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A431 cells with LAMP-1/CD107a Antibody [H4A3] NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0015.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0016.jpg)
![Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.](http://images.novusbio.com/fullsize/LCAT-Antibody-Western-Blot-NBP1-05950-img0003.jpg "Western Blot: LCAT Antibody [NBP1-05950] - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.")
![Immunocytochemistry/Immunofluorescence: LCAT Antibody [NBP1-05950] - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/LCAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05950-img0004.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0007.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.")
![Western Blot: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of different cell lysates using mouse mAb to LAMP1, NBP2-25154 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50uM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody fails to recognize the rodent homologue. The same blot was probed with chicken pAB to HSP60, dilution 1:20,000. The HSP60 antibody reveals heat shock 60 protein with apparent molecular weight of 60kDa in all preparations.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Western-Blot-NBP2-25154-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for immunofluorescent staining of lysosomal (LAMP-1/CD107a, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04070-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0008.jpg)
![Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Western-Blot-NBP2-02396-img0001.jpg "Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunocytochemistry-Immunofluorescence-NBP2-02396-img0008.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0007.jpg)
![Flow Cytometry: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells transfected with either Endothelial Lipase Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Flow-Cytometry-NBP2-02396-img0010.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0002.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0003.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0004.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human breast tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0005.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human Kidney tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0006.jpg)
![Immunoprecipitation: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Lane 1: Negative Control Lane 2: NBP2-02378 Lane 3: NBP2-02380 Lane 4:NBP2-02381 Lane 5: NBP2-02382 Lane 6: NBP2-02394 Lane 7: NBP2-02395 Lane 8: NBP2-02396 Lane 9: NBP2-02397](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunoprecipitation-NBP2-02396-img0009.jpg)
![Western Blot: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Western-Blot-NBP2-59780-img0009.jpg "Western Blot: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human liver shows strong granular cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human fallopian tube shows granular cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human endometrium shows granular cytoplasmic immunoreactivity in epithelial and stromal cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human prostate shows strong granular cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0004.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-118-img0003.jpg "Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-118] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-118-img0002.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-118] - Analysis of Endothelial Lipase in ECV304 cells.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-118-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0006.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 10 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, FITC conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0005.jpg)