| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89203-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45345 | |||
| Azide and BSA Free | NBP2-34733-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34671-0.1mg | |||
![Western Blot: Cathepsin L Antibody (33/2) [NB100-1775] - Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L. The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate and NBT/BCIP substrate. Lane A: Antibody dilution 1:400. Lane B: Negative control (only secondary antibody).](http://images.novusbio.com/fullsize/Cathepsin-L-Antibody-33-2-Western-Blot-NB100-1775-img0001.jpg "Western Blot: Cathepsin L Antibody (33/2) [NB100-1775] - Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L. The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate and NBT/BCIP substrate. Lane A: Antibody dilution 1:400. Lane B: Negative control (only secondary antibody).")
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunofluorescence-NBP1-97725-img0004.jpg "Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunohistochemistry-Paraffin-NBP1-97725-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunocytochemistry-Immunofluorescence-NBP1-97725-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunocytochemistry-Immunofluorescence-NBP1-97725-img0003.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Western-Blot-NB500-383-img0003.jpg "Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0004.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-MEM-101A-Flow-Cytometry-NB500-383-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Frozen-NBP1-97671-img0001.jpg)
![Flow Cytometry: Vimentin Antibody (RV203) [NBP1-97671] - An intracellular stain was performed on HeLa cells with NBP1-97671PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Flow-Cytometry-NBP1-97671-img0003.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - Paraffin section of human colon](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Paraffin-NBP1-97671-img0002.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Figure 3. Immunohistochemistry with NBP1-97671 (RV203) on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-RV203-Immunohistochemistry-Frozen-NBP1-97671-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.A: Ki67, 20X, insert- 5X digital (LCAS-R-12 weeks post-injection) B: Oct3/4, 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) C: Nestin 20x, insert- 5X digital (LCAS-R-12 weeks post-injection) D: Nestin 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) E: Sox2 40X, insert- 5X digital, (LC26-R-12 weeks post-injection) F: Sox2 40X, insert- 5X digital (LCAS-R-12 weeks post-injection) G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-31020241534370.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.A: Ki67, 20X, insert- 5X digital (LCAS-R-12 weeks post-injection) B: Oct3/4, 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) C: Nestin 20x, insert- 5X digital (LCAS-R-12 weeks post-injection) D: Nestin 40x, insert- 5X digital (LCAS-R-12 weeks post-injection) E: Sox2 40X, insert- 5X digital, (LC26-R-12 weeks post-injection) F: Sox2 40X, insert- 5X digital (LCAS-R-12 weeks post-injection) G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-31020241535653.jpg)
![Immunohistochemistry: Vimentin Antibody (RV203) - BSA Free [NBP1-97671] - Analysis of RG, TCL & the TCL derived tumors(A1, A2) LCAS-R 2nd nsphrs/LCAS-RTL(138) 2nd nsphr, (A3) Tubb3 40x LCAS-RTL(138) 2nd nsphr, (A4) GFAP 40x LCAS-RTL(138) 2nd nsphr; (B1, B2) HE 40x LCAS-R/LCAS-RTL(138) - images show undifferentiated small round blue cells with scarce cytoplasm, (B3) HE 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - extensive proliferation of undifferentiated embryonal tumor cells infiltrating the adjacent brain parenchyma, (B4) Ki67 40x (LCAS-RTL(138) SVZ 15 weeks post-injection) - high proliferative activity of tumor cells is reflected by over 75% of cells expressing Ki-67, (C1) OCT3/4 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensive proliferation of tumor cells invading adjacent brain parenchyma show high expression of OCT3/4 (over 80% of cells), (C2) Sox2 40x (LC26-RTL(170) SVZ 12 weeks post-injection) - extensively expressed in the tumor cells, (C3) PRAME 40x (LCAS-R 12 weeks post-injection) - extensively expressed in the tumor cells, (C4) Nestin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells, (D1) Vimentin 40x (LCAS-RTL(138) 15 weeks post-injection) - extensively expressed in the tumor cells within the main tumor mass & in the invading areas, (D2) BLBP 40x (LC26-RTL(170) 16 weeks post-injection) - extensively expressed in the tumor cells, (D3, D4) OTX2 20x, 40x (LC26-RTL(170) 16 weeks post-injection) - extensive expression (over 80% of cells) in tumor cells. TU-tumor; P-parenchyma; PI- perivascular invasion. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.24460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97671_mouse-monoclonal-vimentin-antibody-rv203-310202416114726.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo")
![Immunohistochemistry: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Immunofluorescence staining of human colon epithelium.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-NBP1-97715-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 5 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0005.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human colon](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0002.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of human kidney epithelium](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0001.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of swine liver hepatocytes](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-RGE53-Immunohistochemistry-Frozen-NBP1-97715-img0003.jpg)
![Western Blot: GFAP Antibody (012) [NBP2-89203] - Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 45 kDa.](http://images.novusbio.com/fullsize/GFAP-Antibody-012-Western-Blot-NBP2-89203-img0001.jpg "Western Blot: GFAP Antibody (012) [NBP2-89203] - Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 45 kDa.")
![Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Western-Blot-NB400-149-img0003.jpg "Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.")
![Immunocytochemistry/Immunofluorescence: PDZK1 Antibody [NB400-149] - PDZK1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-149-img0004.jpg)
![Immunohistochemistry: PDZK1 Antibody [NB400-149] - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-NB400-149-img0005.jpg)
![Simple Western: PDZK1 Antibody [NB400-149] - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Simple-Western-NB400-149-img0006.jpg)
![Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP2-25163-img0005.jpg "Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Tau Antibody [NBP2-25163] - Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-tau, NBP2-25163, dilution 1:2,000 in green, and costained with mouse mAb to MAP2, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The NBP2-25163 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.](http://images.novusbio.com/fullsize/Tau-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25163-img0004.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Western-Blot-NBP2-15052-img0013.jpg "Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Paraffin-embedded human cervical carcinoma.Oct4 stained by Oct4 antibody [GT486] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0016.jpg)
![Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Flow-Cytometry-NBP2-15052-img0009.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant OCT3/4 protein 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Western-Blot-NBP2-15052-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cell were fixed in 4% Paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunocytochemistry-Immunofluorescence-NBP2-15052-img0008.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - BT483 xenograft, using OCT3/4 antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0001.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Imaging of Rabbit stem cels in Bone marrow of tibia. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0012.jpg)
![Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunoprecipitation-NBP2-15052-img0010.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Mouse embryonic stem cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (NBP2-15052) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-910202420134071.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.</br>Sample: Paraffin-embedded human breast carcinoma.</br>Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-91020242011495.jpg)
![Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.</br>Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.</br>Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-9102024202468.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody (486) [NBP2-15052] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-1610202419405748.jpg)
![Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.<br />A. 30 ug human ESC whole cell lysate/extract<br />10% SDS-PAGE<br /> (NBP2-15052) dilution: 1:1000 <br />The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-western-blot-2611202518301214.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM123) [NBP2-32933] - Formalin-fixed paraffin-embedded human tonsil stained with CD34 Monoclonal Antibody (SPM123)](http://images.novusbio.com/fullsize/CD34-Antibody-SPM123-Immunohistochemistry-Paraffin-NBP2-32933-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (SPM123) [NBP2-32933] - Formalin-fixed paraffin-embedded human tonsil stained with CD34 Monoclonal Antibody (SPM123)")
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0002.jpg "Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-NBP2-32867-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867] - Immunofluorescent staining of MEOH-fixed HeLa cells using followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunocytochemistry-Immunofluorescence-NBP2-32867-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunohistochemistry-Paraffin-NBP2-32867-img0008.jpg)
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0004.jpg)
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Mtb Rv1468c directly binds to poly-Ub chains in a UBA-dependent manner. a, b Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated w/ (+) or w/out (–) proteinase K followed by incubation w/ K632–7 (a) or K482–7 (b) poly-Ub chains at 4 °C for 4 h. c Confocal microscopy analysis for colocalization of pSC301-Mtb stably expressing green fluorescent protein (GFP) w/ K632–7 or K482–7 Ub chains in vitro. Bacteria (green) treated w/ (+) or w/out (–) proteinase K further incubated w/ poly-Ub chains as in a & b, & then immunostained using anti-Ub antibody (red). Scale bars, 10 μm. The % colocalizations of Ub & Mtb shown at the bottom. A total of 100 bacterial cells counted. Two-tailed unpaired t-test performed. d Schematic representation of Mtb Rv1468c constructs used. e In vitro interaction analysis of Ub & GST-tagged full-length Mtb Rv1468c or the truncated mutants as indicated in d. Purified recombinant GST-tagged Mtb Rv1468c variants immobilized on glutathione sepharose resin. Mono-Ub (His6-tagged), K63 or K48-linked poly-Ub (Ub2–7) added, & then the protein complexes assayed by immunoblotting w/ indicated antibodies. f Immunoblot analysis of proteins immunoprecipitated (IP) w/ anti-Flag M2 Affinity Gel from lysates of HEK293T cells transfected w/ empty vector (–) or hemagglutinin (HA)-tagged Ub (+) & Flag-tagged wild-type (WT) Rv1468c or its mutants (V64G or L65G). g Confocal microscopy analysis of HeLa cells cotransfected w/ vectors encoding HA-tagged Ub (red) & GFP-tagged WT Rv1468c, V64G or L65G mutant (green). Nuclei (blue) stained w/ DNA-binding dye DAPI. Scale bars, 10 μm. Results representatives from at least 3 independent experiments (mean ± s.e.m. of n = 5 in c). The source data used in c provided in Source Data Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31036822), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32867_mouse-monoclonal-hsp60-antibody-lk2-31020241612249.jpg)
![Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Western-Blot-H00009700-M01-img0005.jpg "Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Separase Antibody (6H6) [H00009700-M01] - Analysis of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Immunohistochemistry-Paraffin-H00009700-M01-img0004.jpg)