| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47985-0.1mg | |||
| IHC-Prediluted | NBP2-48056 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85223-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88071-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70204 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71667 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70078 | |||
![Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31350-img0004.jpg "Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunohistochemistry-Paraffin-NBP1-31350-img0005.jpg)
![Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/MCM7-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31350-img0008.jpg)
![Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 antibody [N2C2], Internal. 7.5 % SDS-PAGE. The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunoprecipitation-NBP1-31350-img0007.jpg)
![Western Blot: Rabbit Polyclonal MCM7 Antibody [NBP1-31350] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. MCM7 Antibody (NBP1-31350) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-212202416030..jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518243316.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-141120251843245.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518381712.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518273411.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0007.jpg "Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.")
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-43535-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunoprecipitation-NBP2-43535-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420122076.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014689.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115031.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014664.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115087.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242011459.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-9102024205654.jpg)
![Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-immunocytochemistry-immunofluorescence-261120251823621.jpg)
![Western Blot: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Western-Blot-NBP2-44948-img0013.jpg "Western Blot: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/834).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Immunofluorescent staining of MCF cells using Cytokeratin 18 Antibody (KRT18/834). Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunocytochemistry-Immunofluorescence-NBP2-44948-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0011.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Cytokeratin 18 Antibody (KRT18/834) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Flow-Cytometry-NBP2-44948-img0009.jpg)
![Western Blot: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Western Blot Analysis of A) human stomach B) human intestine using Cytokeratin 18 Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Western-Blot-NBP2-44948-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Lung Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Skin stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) [NBP2-44948] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-Immunohistochemistry-Paraffin-NBP2-44948-img0007.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NQO-1 Antibody [NBP1-85223] - Analysis in human cell lines A-549 and HEK293 using Anti-NQO1 antibody. Corresponding NQO1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-85223-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NBP1-85223] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85223-img0008.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0017.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0015.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0016.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0018.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Analysis in human skeletal muscle and liver tissues. Corresponding TTN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Analysis in human skeletal muscle and liver tissues. Corresponding TTN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Titin Antibody [NBP1-88071] - SMYD2 Cys13 glutathionylation or oxidation reduces myofibril integrity. Monitoring the myofibril alignment in rat neonatal cardiomyocytes upon incubation of AMA (2 ug/mL) for 12 h: no expression of SMYD2 WT. Immunostainings were done by using antibodies to SMYD2, HA (green), or titin (alpha-titin-NT, red). About 30 cells were photographed and examined for myofibril alignment or directionality by FiberFit software. 34 Images represent the major myofibril structure in individual conditions. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-06786-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88071-img0015.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0014.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0011.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0012.jpg)
![Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Titin-Antibody-Immunohistochemistry-Paraffin-NBP1-88071-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Titin Antibody [NBP1-88071] - SMYD2 Cys13 glutathionylation or oxidation reduces myofibril integrity. a, b Monitoring the myofibril alignment in rat neonatal cardiomyocytes upon incubation of AMA (2 µg/mL) for 12 h: no expression (a) & ectopic expression of SMYD2 WT or C13S (b). Immunostainings were done by using antibodies to SMYD2, HA (green), or titin ( alpha -titin-NT, red). About 30 cells were photographed & examined for myofibril alignment or directionality by FiberFit software.34 Images represent the major myofibril structure in individual conditions. Scale bars, 10 µm. c Analysis of myofibril alignment in cardiomyocytes. Individual cell images were analyzed by the FiberFit software to determine the fiber dispersion parameter (k) values that represent the degree of fiber alignment. High k values represent the aligned networks, whereas low k values represent the disordered networks. The median values with 95% CI are shown, n = 3 independent experiments. Difference is significant by one-way ANOVA, followed by Tukey’s post-hoc test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30337525), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88071_rabbit-polyclonal-titin-antibody-310202416121981.jpg)
![Immunocytochemistry/ Immunofluorescence: Titin Antibody [NBP1-88071] - SMYD2 Cys13 glutathionylation induces dissociation of SMYD2 from N2A & Hsp90. a, b SMYD2 glutathionylation disrupts its interaction with Hsp90. Purified SMYD2-SH & SMYD2-SSG were incubated with GST-Hsp90 bound to glutathione beads, & eluted sample was analyzed (a). Hsp90 was co-immunoprecipitated with SMYD2 WT or C13S from HEK293 cells in response to AMA with glucose deprivation (b). c, d SMYD2 glutathionylation disrupts its interaction with N2A. Purified SMYD2-SH & SMYD2-SSG were incubated with GST-N2A bound to glutathione beads, & eluted sample was analyzed (c). FLAG-N2A was co-immunoprecipitated with SMYD2 WT or C13S in HEK293 cells in response to AMA with glucose deprivation (d). e SMYD2 subjected to glutathionylation decreases its binding with N2A. SMYD2 WT or C13S was pre-incubated with H2O2 in the absence or presence of glutathione for 15 min, then mixed with GST-N2A bound to glutathione beads for 1 h. Eluted samples were analyzed. f, g Colocalization of titin & SMYD2 decreases upon incubation of AMA in rat neonatal cardiomyocytes expressing SMYD2 WT versus C13S. Immunostainings of cardiomyocytes with antibodies to titin ( alpha -titin-NT, red), HA, or SMYD2 (green) are shown with enlarged areas for details (the red boxes) (f). Pearson’s correlation coefficients were calculated to determine colocalization of titin & SMYD2 (g). About 30 cells were analyzed in individual conditions. Images represent the major colocalization pattern in individual experiments. Scale bars, 10 µm. Data represent the mean ± SD, n = 3 independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30337525), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88071_rabbit-polyclonal-titin-antibody-310202416114738.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0009.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Staining of paraffin-embedded Human liver tissue using anti-liver Arginase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Immunohistochemistry-Paraffin-NBP2-03618-img0006.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-liver Arginase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Flow-Cytometry-NBP2-03618-img0005.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver Arginase.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0007.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0008.jpg)
![Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KIF2C monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Western-Blot-NBP2-01064-img0010.jpg "Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KIF2C monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KIF2C .](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunocytochemistry-Immunofluorescence-NBP2-01064-img0011.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Human tonsil using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0009.jpg)
![Flow Cytometry: KIF2C Antibody (OTI1C5) [NBP2-01064] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KIF2C antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Flow-Cytometry-NBP2-01064-img0001.jpg)
![Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF2C.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Western-Blot-NBP2-01064-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0003.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0004.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0005.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0006.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0007.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Human Kidney tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0008.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0020.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0016.jpg)
![Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Western-Blot-NBP1-48251-img0018.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0012.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0014.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and ACTN1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ACTN1 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Knockout-Validated-NBP1-48251-img0021.jpg)
![Simple Western:alpha-Actinin 1 Antibody (OTI7A4)[NBP1-48251]Simple Western� analysis of endogenous protein Cytokeratin 8 from HELA lysates (0.5 mg/mL) using Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp1-48251_mouse-alpha-actinin-1-mab-oti7a4-simple-western-13112025123690.jpg%253fstore=america&image-type=image)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0001.jpg "Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.")
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of His-tagged protein in E. coli lysates. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0012.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 680 conjugate of NBP2-31055. Detection of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0021.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0013.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 ug/mL).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0014.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of recombinant Treponema denticola major outer sheath His-tagged protein fragments (rMSP, rMSP-C, rMSP-V, rMSP-N) purified from E. coli was performed by loading 5 ug of per well. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 488 conjugate of NBP2-31055. Staining of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a DyLight 488-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scietific ArrayScan or ToxInsight Instrument at 20X magnification.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0024.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (D33) [NB120-17156] - Formalin fixed paraffin embedded human leiomyoma stained with Desmin antibody (NB120-17156).](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-Paraffin-NB120-17156-img0009.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (D33) [NB120-17156] - Formalin fixed paraffin embedded human leiomyoma stained with Desmin antibody (NB120-17156).")
![Immunohistochemistry-Paraffin: Desmin Antibody (D33) [NB120-17156] - Paraffin embedded human leiomyoma stained with Desmin antibody.](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-Paraffin-NB120-17156-img0007.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (D33) [NB120-17156] - FFPE human leiomyoma.](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-Paraffin-NB120-17156-img0002.jpg)
![Immunohistochemistry: Desmin Antibody (D33) [NB120-17156]](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-NB120-17156-img0003.jpg)
![Immunohistochemistry: Desmin Antibody (D33) [NB120-17156]](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-NB120-17156-img0004.jpg)
![Immunohistochemistry: Desmin Antibody (D33) [NB120-17156]](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-NB120-17156-img0005.jpg)
![Immunohistochemistry: Desmin Antibody (D33) [NB120-17156]](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-NB120-17156-img0006.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (D33) [NB120-17156] - Rat heart tissue, alcohol fixed, paraffin embedded. Image from verified customer review.](http://images.novusbio.com/fullsize/Desmin-Antibody-D33-Immunohistochemistry-Paraffin-NB120-17156-img0008.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0008.jpg "Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0010.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0009.jpg)
![Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Western blot analysis of Cytokeratin 18 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Western-Blot-NBP2-67370-img0011.jpg "Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Western blot analysis of Cytokeratin 18 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0016.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Flow-Cytometry-NBP2-67370-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0013.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon carcinoma with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0015.jpg)