| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP2-46876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72740 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43359-0.05mg | |||
| BSA Free | NBP1-43359-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64988SS | |||
| Azide and BSA Free | NBP2-80647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54503-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47946-0.1mg | |||
| IHC-Prediluted | NBP2-48095 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47842-0.1mg | |||
| IHC-Prediluted | NBP2-48357 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82245-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| Azide and BSA Free | NBP2-80765 | |||
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NB500-161-img0008.jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KAT3B/p300 Antibody [NB500-161] - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0010.jpg)
![KAT3B/p300 Antibody [NB500-161] - Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for IP at 6 ug/mg lysate. p300 was also immunoprecipitated by a previous lot (lot NB500-161-1) of this antibody. For blotting immunoprecipitated p300, NB500-161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-NB500-161-img0011.jpg)
![Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.](http://images.novusbio.com/fullsize/Collagen-II-Antibody-Immunohistochemistry-NBP1-77795-img0005.jpg "Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.")
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0001.jpg "Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.")
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of His-tagged protein in E. coli lysates. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0012.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 680 conjugate of NBP2-31055. Detection of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0021.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0013.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 ug/mL).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0014.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of recombinant Treponema denticola major outer sheath His-tagged protein fragments (rMSP, rMSP-C, rMSP-V, rMSP-N) purified from E. coli was performed by loading 5 ug of per well. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 488 conjugate of NBP2-31055. Staining of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a DyLight 488-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scietific ArrayScan or ToxInsight Instrument at 20X magnification.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0024.jpg)
![Western Blot: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Western-Blot-NBP2-45887-img0008.jpg "Western Blot: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.")
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MMP13.](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Immunocytochemistry-Immunofluorescence-NBP2-45887-img0010.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-45887-img0016.jpg)
![Western Blot: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of extracts (10ug) from 5 different cell lines.](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Western-Blot-NBP2-45887-img0009.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-45887-img0011.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-45887-img0012.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-45887-img0013.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-45887-img0014.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (2D8) [NBP2-45887] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-45887-img0015.jpg)
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable c](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0001.jpg)
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0002.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0008.jpg "Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.")
![Flow (Cell Surface): CD4 Antibody (W3/25) [NB100-64988] - Rat Splenocytes were stained with NB100-64988 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alex Fluor 488.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Flow-Cell-Surface-NB100-64988-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody (W3/25) [NB100-64988] - Staining of rat splenocytes with CD4 (W3/25) antibody. Live cells were incubated with CD4 antibody on ice, then fixed with 2% formaldehyde prior to secondary antibody incubation.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Immunocytochemistry-Immunofluorescence-NB100-64988-img0007.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody (W3/25) [NB100-64988] - Staining of frozen rat spleen with Mouse anti Rat CD4.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Immunohistochemistry-Frozen-NB100-64988-img0001.jpg)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Staining of stimulated rat spleen cells.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0003.jpg)
![Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) [NBP2-53383] - Formalin-fixed, paraffin-embedded human Uterus stained with Desmin Monoclonal Antibody (DES/1711)](http://images.novusbio.com/fullsize/Desmin-Antibody-DES-1711-Immunohistochemistry-Paraffin-NBP2-53383-img0001.jpg "Immunohistochemistry-Paraffin: Desmin Antibody (DES/1711) [NBP2-53383] - Formalin-fixed, paraffin-embedded human Uterus stained with Desmin Monoclonal Antibody (DES/1711)")
![Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Western-Blot-NBP2-44827-img0012.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (KRT19/800) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunocytochemistry-Immunofluorescence-NBP2-44827-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Colon stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Lung stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Stomach stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0008.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin gamma 1 Antibody (A5).](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-A5-Immunohistochemistry-Paraffin-NBP2-44751-img0003.jpg "Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin gamma 1 Antibody (A5).")
![Immunohistochemistry-Frozen: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-A5-Immunohistochemistry-Frozen-NBP2-44751-img0002.jpg)
![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (A5).](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-A5-Immunohistochemistry-Paraffin-NBP2-44751-img0001.jpg)
![Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0006.jpg](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0006.jpg "<b>Genetic Strategies Validation. </b>Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0006.jpg")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Tau [p Thr181] Antibody [NB100-82245] - analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr181) antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Immunohistochemistry-Paraffin-NB100-82245-img0005.jpg)
![Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Analysis of extracts from mouse brain tissue using Tau(Phospho-Thr181) Antibody and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0001.jpg)
![Simple Western: Tau [p Thr181] Antibody [NB100-82245] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Simple-Western-NB100-82245-img0004.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Western-Blot-NBP2-75978-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Knockout-Validated-NBP2-75978-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Immunocytochemistry-Immunofluorescence-NBP2-75978-img0002.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (2443C) [NBP2-75978] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte (TM) HRP Polymer Antibody (Catalog # VC002). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Immunohistochemistry-Paraffin-NBP2-75978-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0007.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) [NBP2-75978] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75978 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Simple-Western-NBP2-75978-img0008.jpg)