| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89388-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96140SS | |||
| Azide and BSA Free | NBP2-80599 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27406SS | |||
| Azide and BSA Free | NBP2-80834 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44973 | |||
| Azide and BSA Free | NBP2-34621-0.1mg | |||
![Immunocytochemistry/Immunofluorescence: S100A6 Antibody [NBP1-89388] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89388-img0018.jpg "Immunocytochemistry/Immunofluorescence: S100A6 Antibody [NBP1-89388] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0022.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0019.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules and in glomeruli.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0020.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0008.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0009.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0003.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on NIH3T3 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0012.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0005.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0007.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U937 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0011.jpg)
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Western-Blot-NBP2-27406-img0009.jpg "Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0008.jpg)
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Western-Blot-NBP2-27406-img0001.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands, whereas the surrounding fibromuscular stroma cells did not develop any staining.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0002.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0003.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0004.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0006.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0007.jpg)
![Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Western-Blot-NB100-74451-img0017.jpg "Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in Human fibroblast cells. Methanol fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0016.jpg)
![Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of 10ug nuclear extract from fractionated mouse tissue lysate.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Western-Blot-NB100-74451-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (red) in HeLa cells at high magnification.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in U251 Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in HMVEC Cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in A549 Cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0015.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.")
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Western-Blot-NB300-560-img0004.jpg "Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg)
![Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Western-Blot-NBP2-22152-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22152-img0001.jpg)
![Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-NBP2-22152-img0002.jpg)
![Western Blot: HDAC5 Antibody [NBP2-22152] - WB analysis of HDAC5 in 1. HeLa, 2. Ntera 2, 3. HepG2 and 4. MCF7 cell lysates.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Western-Blot-NBP2-22152-img0003.jpg)
![Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/IGF-I-Antibody-Immunohistochemistry-Paraffin-NBP2-16929-img0003.jpg "Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0046.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-NB100-456-img0044.jpg)
![Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.](http://images.novusbio.com/fullsize/PCNA-Antibody-Flow-Cytometry-NB100-456-img0004.jpg)
![Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0041.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0043.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NB100-456-img0047.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0010.jpg "Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Co-localization of necdin and CCAR1 in primary myoblasts of wt (C57/Bl6) mice. Images taken at confocal laser scanning microscope at 40x (i-v) and 63x (zoomed 1.67 times) magnification. Nuclei are stained with DAPI (i, vi; blue). Panels shows immunostaining experiment using the polyclonal anti-CCAR1 (ii, vii, Ab-CCAR1-red) or monoclonal anti-Ndn (iii, viii; Ab-Ndn-green). Panel iv shows the merged images of ii and iii and panel ix the merged images of vii and viii (Ab-CCAR1 + Ab-Ndn-yellow). Panel v shows the merged images of i, ii and iii and panel x the merged images of vi, vii, viii, (Ab-CCAR + Ab-Ndn + DAPI). Scale bar (i-iv) = 17 um; (vi-x) = 41,75 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0011.jpg)
![Immunohistochemistry-Paraffin: CCAR1 Antibody [NB500-186] - Human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-CCAR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunohistochemistry-Paraffin-NB500-186-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human CCAR1on HeLa whole cell lysate using NB500-186. CCAR1 was also IPed with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. IPed CCAR1 was blotted using NB500-188.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human and Mouse CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0006.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Interaction of necdin and CCAR1 in C2C12 myoblasts and primary myoblast cells. Co-IP experiments were performed on protein extract from C2C12 transfected with pCMV-FLAG-Necdin (C2C12 pCMV-FLAG-Ndn) or primary myoblasts from C57/Bl6 newborn mice. Proteins were immunoprecipitated using the polyclonal anti-Ndn (alpha-Ndn) or polyclonal anti-CCAR1 (alpha-CCAR1) and with a non-specific rabbit antisera as control (IgG). Necdin and CCAR1 were detected in immunoprecipitated samples using specific antibody. Input (I) represents 10% of the immunoprecipitated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0008.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing CCAR1 and necdin (beta-actin as loading control) expression in C2C12 mock transfected (M) or transfected with pSG5-HA-CCAR1 and/or pCMV-FLAG-Necdin (N or N+C). Both the endogenous and transfected CCAR1 were detected with the polyclonal anti-CCAR1, the transfected CCAR1 with anti-HA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Images taken at confocal laser scanning microscope (63x magnif. zoomed 1.67 x) showing co-immunostaining on C2C12 transfected with pSG5-HA-CCAR1 (i) and/or pCMV-Ndn (C2C12, CCAR1-Ndn-N+C) (ii, iii) and on primary myoblasts from Ndn-/- (Ndn-/-) (iv) and tgMlcNec (MlcNec) (v) newborn mice. Panels i-v show immunostaining using the specific monoclonal anti-Ndn (Ndn: Ab-Ndn-green); panels i'-v' show immunostaining using the polyclonal anti-CCAR1 (CCAR1: Ab-CCAR1-red). Co-immunostained images of anti-Ndn and anti-CCAR1 are shown in panels i''-v'' (Ab-CCAR1 + Ab-Ndn-yellow). Panels i'''-v''' show merged images with nuclei stained with DAPI (merge Ab-CCAR1 + ab-Ndn + DAPI). Scale bars (i-iii) 41,75 um; (iv-v) 44,7 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0012.jpg)
![Immunoprecipitation: CCAR1 Antibody [NB500-186] - Detection of human CCAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 (lot NB500-186-2) used for IP at 6 ug/mg lysate. CCAR1 was also immunoprecipitated by a previous lot (lot NB500-186-1) of the antibody. For blotting immunoprecipitated CCAR1, NB500-186 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunoprecipitation-NB500-186-img0007.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416182617.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416165639.jpg)
![Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Western-Blot-NBP2-32972-img0008.jpg "Western Blot: HSP27 Antibody (G3.1) [NBP2-32972] - Western Blot Analysis of HeLa cell lysate using HSP27 Antibody (G3.1).")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (G3.1) [NBP2-32972] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunocytochemistry-Immunofluorescence-NBP2-32972-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0004.jpg)
![Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Flow-Cytometry-NBP2-32972-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)](http://images.novusbio.com/fullsize/HSP27-Antibody-G3-1-Immunohistochemistry-Paraffin-NBP2-32972-img0003.jpg)
