| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13294-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90243-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86863-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87262-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33066 | |||
| IHC-Prediluted | NBP2-44365 | |||
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Analysis in human placenta and skeletal muscle tissues. Corresponding TOP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0015.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human placenta shows very strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0018.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0019.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0016.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody [NBP1-90365] - Staining of human lymphoid tissues shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90365-img0017.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Septin-9 Antibody [NBP2-13294] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using anti-SEPT9 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP2-13294-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP2-13294] - Staining of human cell line A-431 shows localization to actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13294-img0006.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0009.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0010.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0011.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Borealin Antibody [NBP1-89951] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89951-img0010.jpg "Immunocytochemistry/Immunofluorescence: Borealin Antibody [NBP1-89951] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0015.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human duodenum shows strong nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0012.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human lymphoid tissues shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0013.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416113528.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416121943.jpg)
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP1-86863] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86863-img0009.jpg "Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP1-86863] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.")
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP1-86863-img0020.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP1-86863-img0017.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP1-86863-img0018.jpg)
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP1-86863-img0019.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Analysis in human placenta and skeletal muscle tissues. Corresponding CDK6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Analysis in human placenta and skeletal muscle tissues. Corresponding CDK6 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cdk6 Antibody [NBP1-87262] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP1-87262-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk6 Antibody [NBP1-87262] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87262-img0019.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0027.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cdk6 Antibody [NBP1-87262] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP1-87262-img0020.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0024.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0025.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0026.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0021.jpg "Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Flow-Cytometry-NBP2-02272-img0019.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0017.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0020.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0002.jpg "Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody [NB100-1199] - LPS/IFN gamma-stimulated neuron-glial co-cultures (Glia/PAL31+ neuron) showing expression of both iNOS and PAL31. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1199-img0006.jpg)
![Immunohistochemistry: ANP32B Antibody [NB100-1199] - (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) and 1000X (E&F). Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-NB100-1199-img0005.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - (0.1 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0001.jpg)
![Immunohistochemistry-Paraffin: ANP32B Antibody [NB100-1199] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-Paraffin-NB100-1199-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ANP32B Antibody [NB100-1199] - Western blot analysis of pal31 siRNA-treated C6 showing knockdown of PAL31 expression by PAL31 silencer using 41.5 (lane1), 83 (lane2), and 166 (lane 3) picomole of pal31 siRNA or 332 picomole negative control. Actin works as a loading control. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Knockdown-Validated-NB100-1199-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Upregulated PAL31 expression was observed not only in microglia/ macrophage but also in glial cells near the injury site. (A) Normal thoracic spinal cord section showing few ED-1 positive cells (red). (B) Normal thoracic spinal cord section showing GFAP positive cells (red). (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) & 1000X (E&F). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-3102024168397.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Overexpression of PAL31 in C6 did not affect the cell proliferative activity. (A) GFP/bright field-superimposed micrograph in GFP overexpressing cells showing > 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-310202415525215.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - Effect of overexpression or knockdown of PAL31 in C6 on H2O2-induced toxicity. (A) Representative micrographs showing C6 overexpressing GFP or GFP-tagged PAL31 after being treated with H2O2 (0 ~ 1 mM) for 4 hours. (B) MTT assay in GFP- or GFP-PAL31-overexpressed C6 after H2O2 treatment showing significant difference (cytoprotective effect of PAL31) at 0.5 mM & 1 mM H2O2 treatment between C6/GFP & C6/PAL31 groups. The data in each dosage were analyzed by two-way ANOVA & Bonferroni post hoc test. *P 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-31020241682198.jpg)
![Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Western-Blot-NBP2-29439-img0011.jpg "Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0010.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Mouse Monoclonal Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0012.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin paraffin breast cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0003.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-paraffin ovarian cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0004.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0008.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0009.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31350-img0004.jpg "Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-31350] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-31350] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunohistochemistry-Paraffin-NBP1-31350-img0005.jpg)
![Chromatin Immunoprecipitation (ChIP): MCM7 Antibody [NBP1-31350] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit igG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.](http://images.novusbio.com/fullsize/MCM7-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31350-img0008.jpg)
![Immunoprecipitation: MCM7 Antibody [NBP1-31350] - MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 antibody [N2C2], Internal. 7.5 % SDS-PAGE. The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/MCM7-Antibody-Immunoprecipitation-NBP1-31350-img0007.jpg)
![Western Blot: Rabbit Polyclonal MCM7 Antibody [NBP1-31350] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. MCM7 Antibody (NBP1-31350) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-212202416030..jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518243316.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-141120251843245.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518381712.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM7 antibody [N2C2], Internal (NBP1-31350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31350_rabbit-polyclonal-mcm7-antibody-western-blot-1411202518273411.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse brain. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunohistochemistry-Paraffin-NBP2-15840-img0012.jpg)
![Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - A431 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FZR1/CDH1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Western-Blot-NBP2-15840-img0013.jpg)
![Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Mouse heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunohistochemistry-Paraffin-NBP2-15840-img0010.jpg)
![Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Rat heart. FZR1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FZR1-CDH1-Antibody-Immunohistochemistry-Paraffin-NBP2-15840-img0011.jpg)
![Western Blot: CD81 Antibody [NBP2-20564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20564_rabbit-polyclonal-cd81-antibody-910202420131279.jpg "Western Blot: CD81 Antibody [NBP2-20564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CD81 Antibody [NBP2-20564] - CD81 Antibody Immunohistochemical analysis of paraffin-embedded Lung Papillary Adenocarcinoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunohistochemistry-Paraffin-NBP2-20564-img0004.jpg)
![Western Blot: CD81 Antibody [NBP2-20564] - AsPC-1 whole cell extracts (5.5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20564_rabbit-polyclonal-cd81-antibody-91020242021979.jpg)
![Western Blot: CD81 Antibody [NBP2-20564] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20564_rabbit-polyclonal-cd81-antibody-91020242011478.jpg)
