Cell-Cycle-and-Replication reagents: Novus Biologicals

NB600-336

c-Myc Antibody - BSA Free

(3 Reviews)

(29 Publications)

0.1 ml

NB600-336

Western Blot: c-Myc Antibody [NB600-336] - Analysis of c-Myc on Jurkat whole cell extract using NB600-336.

Western Blot: c-Myc Antibody [NB600-336] - Lane 1: control HEK293 cell lysate. Lane 2: CMYC over-expression in HEK293 cell lysate. WB image submitted by a verified customer review.

Immunohistochemistry: c-Myc Antibody [NB600-336] - Analysis of c-Myc in mouse prostate using DAB with hematoxylin counterstain.

Western Blot: c-Myc Antibody [NB600-336] - c-Myc in MOLT-4 cells. WB image submitted by a verified customer review.

Western Blot: c-Myc Antibody [NB600-336] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-cMyc antibody (NB600-336) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of c-Myc antibody bound to the blotted protein. This c-Myc antibody detected the c-Myc specific band at its expected position (48-50kDa). The signal below 10 kDa in this blot is potentially the degraded protein and we have not characterized this band.

Western Blot: c-Myc Antibody [NB600-336] - Analysis using the HRP conjugate of NB600-336. Detection of c-Myc in NIH/3T3 cell lysates (30ug and 45ug per lane) using anti-c-Myc antibody. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-336] - Detection of c-myc Tagged Plakoglobin by Immunofluorescence. Samples: Human microvascular endothelial cells expressing c-myc tagged plakoglobin following transient transfection.

Simple Western: c-Myc Antibody [NB600-336] - Lane view shows a specific band for c-Myc in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Z363 promotes MYC and TAF10 degradation. (A) Identification of small inhibitory molecules for MYC. (B) MCF7 cells were treated with Z363 (0, 2.5, 7.5 and 15 μg/ml) for 24 h. The protein levels of MYC and TAF10 were analysed by Western blotting. (C) MCF7 cells were treated with Z363 (7.5 μg/ml) for 0, 6, 12 and 24 h. Furthermore, the protein levels of MYC and TAF10 were analysed by Western blotting. (D) MCF7 cells were treated with 25 μM MG132 at the indicated time points, followed by treatment with or without Z363 (7.5 μg/ml) for 24 h, and MYC and TAF10 expressions were analysed by Western blotting. (E) Western blots for MYC, phosphorylated MYC T58 and S62 in MCF7 cells treated with Z363 at the times indicated. (F) Ratios of pT58 or pS62 to total MYC protein levels from the experiment (E). (G) IF staining for Ki67 and pT58 in Z363‐treated MCF7 cells, scale bar, 10 μm. (H) MCF7 cells were treated with 25 μM MG132 for 2 h, followed by Z363 treatment (7.5 μg/ml) for 24 h. Expressions of MYC and TAF10 were assessed using Western blot analysis. Data shown in F were analysed by two‐way ANOVA. Fluorescence images and blots were representative of three independent experiments. All data are presented as the mean +/- SEM of n = 3. ***p < .001, ns, no significance Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36639831), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The role of miR124-3p and miR766-3p target genes in HNSCC drug resistance. (A) Expression analysis of miR124-3p and miR766-3p direct target genes and downstream target genes by Western blot in HNSCC cell lines (CAL27 and FaDu), with or without transfection with miRNA inhibitors or miRNA mimics. Left: Western blotting showed the expression of miR124-3p target gene (CREBRF) and CREBRF target genes (ATG5 and CREB3). Right: Western blotting showed the expression of miR766-3p target gene (NR3C2) and NR3C2 target genes ( beta -catenin and c-Myc). Quantitative data (relative expression levels after beta -actin-corrected) from three independent experiments are disclosed below each protein band. Data represent the mean +/- SD (n = 3). (B) Target gene analysis in sensitive (CAL27) vs. resistant (CAL27/FP-R) HNSCC cell lines. Quantitative data (relative expression levels after beta -actin-corrected) is shown below each protein band. (C) The effect of NR3C2 and/or CREBRF knockdown on drug-induced cytotoxicity in CAL27 and FaDu. Cells were transfected by 10 nM siRNA (single or combined) for 24 h followed by 72 h exposure to the indicated drug. Cytotoxicity was determined by MTT assay. IC50 values are listed in Table S7. (D) Measurement of apoptosis in CAL27 or FaDu cells in response to cisplatin or 5-FU +/- 24 h prior transfection with 10 nM siRNA. After 24 h of drug treatments, cells were labeled with anti-annexin V-FITC antibody and PI and then analyzed with flow cytometry. A two-way ANOVA with Bonferroni’s correction for multiple comparisons was used to analyze group comparisons, and data are presented as means +/- SD (n = 3). * p < 0.05, *** p < 0.001 (vs. control siRNA), and ### p < 0.001 (vs. untreated). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36358691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

TAF10 promotes cancer cell proliferation and migration. (A and B) MCF7 cells were transfected with Flag‐labelled MYC and HA‐labelled TAF10, and the interaction between MYC and TAF10 was detected by Co‐IP. (C) Endogenous interaction of MYC and TAF10 was tested in MCF7 cells. (D) Schematic representation of MYC mutants. (E) Schematic representation of TAF10 mutants. Co‐IP was used to detect the interaction between the TAF10 mutants and MYC in MCF7 cells. Co‐IP was used to detect the interaction. (G) Interaction between the MYC mutants and TAF10 in MCF7 cells was detected using Co‐IP. (H) MCF7 cells were co‐transfected with empty vector (Vec) and HA‐labelled TAF10 wild‐type (HA‐TAF10 WT) or mutants (HA‐TAF10 delta 1‐116, HA‐TAF10 delta 116‐206, HA‐TAF10 delta 206‐218). The MYC promoter activity was analysed using the dual luciferase reporter assay 24 h later. (I) Overexpression of TAF10 assessed the capacity for colony formation. MCF7 cells were transfected with either Control‐vec or HA‐TAF10. The ability of cells to form colonies was measured using crystal violet staining. (J) Overexpression of TAF10 measured the migration capacity of cells. MCF7 cells were transfected with either Control‐vec or HA‐TAF10. Transwell assays were used to assess the migration capacity of cells. Scale bar, 20 μm. Data shown in H were analysed by one‐way ANOVA. Data shown in I and J were analysed by t‐test. The blots represented three independent experiments. All data are presented as the mean +/- SEM of n = 3. ***p < .001, **p < .01, ns, no significance Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36639831), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Co‐inhibition of MYC and TAF10 causes synergistic reduction of cell proliferation and tumour growth. (A) Cells (WT, TAF10 KO) were treated with different doses of Z363 for 24 h. Cell proliferation was determined by the CCK‐8 assay. (B) Knockout reliability was detected by Western blotting. (C) The proliferation of wild‐type cells (WT), single and double KO cells (MYC KO, TAF10 KO, DKO) and Z363‐treated cells were detected by Ki67 ELISA kit. (D) The apoptosis of wild‐type cells (WT), single and double KO cells (MYC KO, TAF10 KO, DKO) and Z363‐treated cells were detected by flow cytometry. (E) Cell apoptosis‐related proteins levels were detected by Western blotting. (F) The migration of wild‐type cells (WT), single and double KO cells (MYC KO, TAF10 KO, DKO), and Z363‐treated cells were detected by a Transwell migration assay. Scale bar, 20 μm. (G) Representative images showing xenograft tumours at day 28 post‐subcutaneous injection (n = 5). (H and I) Tumours were measured and depicted as tumour volume (H) or tumour weight (I). Data shown in A were analysed by two‐way ANOVA. Data shown in C, D, F, H and I were analysed by one‐way ANOVA. Flow cytometry, transwell and blots were representative of three independent experiments. All data are presented as the mean +/- SEM of n = 3. ***p < .001, **p < .01, *p < .05 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36639831), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Kar4p interacts with the MIS complex components Mum2p and Slz1p.(A) Western blots of total protein and co-IPs between Mum2p-4MYC and the alleles of Kar4p-3HA. (MY 16256 and 16257) (Top) Total protein samples from the extracts that were used for the Co-IPs. Kar2p is used as a loading control. Alleles proficient for Kar4p’s meiotic function (Mei+) are in blue and alleles in red are defective (Mei-). (Bottom) Co-IPs where Mum2p-4MYC was purified and the co-purification of Kar4p-3HA was assayed. (B) Western blots of total protein and Co-IPs between Slz1p-3HA and Kar4p-9MYC. (MY 16405 and 16409) (Left) Total protein samples from the extracts that were used for the co-IPs. “*

Kar4p is required for mRNA m6A methylation.(A) mRNA m6A levels measured using an ELISA-like assay. The indicated mutations were made in the SK1 strain background (MY 16325, 16326, 16351, 16353, and 16356) and samples were harvested after four hours of exposure to meiosis inducing conditions. Experiments were run in three biological replicates for each strain and error bars represent standard deviation. (B) Western Blot of 3xFLAG-Rme1p in wild type (MY 16563) and kar4 delta / delta (MY 16569) across a time course of meiosis. Kar2p is used as a loading control. (C) Western blot of 3xMYC-Ime4p after four hours in meiosis inducing media with either 100 μM cycloheximide or an equivalent amount of DMSO in both wild type (Say914) and kar4 delta / delta (MY 16543). (Top) 3xMYC-Ime4p levels with DMSO. (Middle) 3xMYC-Ime4p levels with cycloheximide. (Bottom) Quantification of three biological replicates of the cycloheximide chase experiment. The strains used are in the SK1 background. Kar2p is used as a loading control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37603553), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Downregulation of CREBRF and NR3C2 increase poor prognosis in HNSCC. (A) Histological analysis of miR124-3p and miR766-3p target gene expression (CREBRF-ATG5/CREB3, NR3C2-beta -catenin/c-Myc) in Responder vs. Non-Responder HNSCC clinical samples. Magnification, x100. Scale bar, 210 um. The quantitative data from all specimens are shown in the bar chart. Each dot in the graph represents an individual clinical sample. Two-sided unpaired Student t test was used to analyze comparisons, and data are presented as means +/- SEM. * p < 0.05 and *** p < 0.001. (B) Histological analysis of tumor morphology in relation to miR124-3p and miR766-3p target gene expression. Representative images of CREBRF, ATG5, CREB3, NR3C2, beta -catenin, and c-Myc expression in the serial section of responder and non-responder HNSCC specimens. BV: Blood Vessel. T: Tumor. N: Normal tissue. The invasive cancer cells are indicated by red arrowhead. Magnification, ×200. Scale bar, 100 um. (C) Summary of resistance mechanisms regulated by miR124-3p and miR766-3p. Our data indicated that upon acquired resistance in HNSCC cells or in non-responder HNSCC tumors, the levels of miR124-3p and miR766-3p go up, which in turn down-regulate its direct target genes: CREBRF and NR3C2, and consequently the expression of downstream targets of CREBRF (ATG5/CREB3) and NR3C2 ( beta -catenin/c-Myc) increased in resistant tumors, which are positively correlated with poor prognosis. Thus, by enhancing the CREBRF-ATG5/CREB3 and NR3C2-beta -catenin/c-Myc axis, miR124-3p and miR766-3p support aggressive HNSCC progression. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36358691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, I, Y

Species Glossary

Reactivity: Hu, Mu, I, Y

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-336AF350
Alexa Fluor 405	NB600-336AF405
Alexa Fluor 488	NB600-336AF488
Alexa Fluor 532	NB600-336AF532
Alexa Fluor 594	NB600-336AF594
Alexa Fluor 647	NB600-336AF647
Alexa Fluor 700	NB600-336AF700
Alexa Fluor 750	NB600-336AF750
Allophycocyanin	NB600-336APC
Biotin	NB600-336B
CoraFluor 1	NB600-336CL1
DyLight 350	NB600-336UV
DyLight 405	NB600-336V
DyLight 488	NB600-336G
DyLight 550	NB600-336R
DyLight 594	NB600-336DL594
DyLight 650	NB600-336C
DyLight 680	NB600-336FR
DyLight 755	NB600-336IR
FITC	NB600-336F
HRP	NB600-336H
Janelia Fluor 525	NB600-336JF525
Janelia Fluor 549	NB600-336JF549
Janelia Fluor 646	NB600-336JF646
Janelia Fluor 669	NB600-336JF669
PE	NB600-336PE
PerCP	NB600-336PCP
mFluor Violet 450 SE	NB600-336MFV450
mFluor Violet 500 SE	NB600-336MFV500
mFluor Violet 610 SE	NB600-336MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-336SS

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NB100-220

ATM Antibody (5C2) - Azide and BSA Free

(36 Publications)

0.1 ml

NB100-220

Western Blot: ATM Antibody (5C2) [NB100-220] - Absence of Atm does not relieve the selective pressure to inactivate p53 during chemical carcinogenesis. Examples of cancer cell lines established from 3MC-fibrosarcomas (each line derives from an independent fibrosarcoma). The genotype of the mice where the 3MC-fibrosarcomas were generated is indicated, as well as, the status of p53 as determined by a nutlin-sensitivity assay (see Supplementary Figures S5 and S6). Cell lines were exposed to 10 Gy and protein extracts were obtained 1h and 24h after irradiation. The levels of the indicated proteins were determined by immunoblotting using beta-actin as loading control. Image collected and cropped by CiteAb from the following publication (www.dx.plos.org/10.1371/journal.pone.0005475) licensed under a CC-BY license.

Immunohistochemistry: ATM Antibody (5C2) [NB100-220] - Limited role of Atm in p53-mediated tumor suppression & DNA damage response in chemically-induced fibrosarcomas.A. Mice of the indicated genotypes, wt (n = 15), p53-super (n = 16), Atm-null (n = 9), p53-super/Atm-null (n = 13) & Arf-null (n = 12), were injected intramuscularly with 3-methyl-cholanthrene (3MC) & tumour development was monitored. Kaplan-Meier tumour-free curves were obtained & statistical significant differences (logrank test) were found for wt vs. p53-super (p<0.005), Atm-null vs. p53-super/Atm-null (p<0.001) & Arf-null vs. wt (p<0.0001). No significant differences were found for wt vs. Atm-null (p = 0.11), or p53-super vs. p53-super/Atm-null (p = 0.18). B. Quantification of the persistent DNA damage response ( gamma H2AX), proliferation (Ki67), & apoptosis (activated caspase-3) in 3MC-fibrosarcomas generated in mice of the indicated genotypes. Quantifications are relative to the 3MC-fibrosarcomas in wild-type mice. Values correspond to the average & standard deviation (n = 5 per genotype). Examples of the immunostainings are shown in the panels at the right. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19421407), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ATM Antibody (5C2) [NB100-220] - BAG3P209L aggregation leads to co-aggregation of proteasomal substrates. a Immunofluorescence pictures of HeLa cells expressing FLAG-BAG3WT or FLAG-BAG3P209L using antibodies against BAG3 (green) or ubiquitin (red). Scale bar = 5 μm. b Suppression of GFP-HttQ74 aggregation of cells expressing a control, FLAG-BAG3WT or FLAG-BAG3P209L. Western blot against indicated antibodies is shown. c Quantification of GFP-HttQ74 aggregation of experiments similar to b. Relative percentage of SDS-insoluble protein levels are shown. Data represents the mean & standard deviation of three independent experiments. d Immunofluorescence pictures of HeLa cells expressing FLAG-BAG3WT or FLAG-BAG3P209L using antibodies against BAG3 (green) or ubiquitin (red). Left column BAG3, middle column ubiquitin & right column is the merge of BAG3 (green), ubiquitin (red), & DAPI (blue). Scale bar = 5 μm. e Fractionation of HEK293 cells expressing HSPB8, a control or BAG3WT or BAG3P209L, together with either Ub-R-GFP or GFP-ODC (ornithine decarboxylase). Western blot against GFP, FLAG (BAG3), Myc (HSPB8), & tubulin are shown. f Fractionation of HEK293 cells expressing HSPB8 & indicated BAG3 variants. Western blot using ubiquitin (FK2) & tubulin antibodies are shown. The same samples as in Fig. 5e have been used, loading control is therefore the same. Source data are provided as a Source data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30559338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB100-306

ATM [p Ser1981] Antibody (10H11.E12) - BSA Free

(1 Review)

(30 Publications)

0.1 ml

NB100-306

Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - Human lymphoblastoid cells stained with ATM [p Ser1981] Antibody (10H11.E12). ICC/IF image submitted by a verified customer review.

<b>Biological Strategies Validation. </b>Western Blot: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - MCELNs decreased the DNA damage of H9C2 cells after radiation. Western blot images of p-ATM (NB100-306) and ATM (NB100-309) in H9C2 cells after 48 h of culture with indicated treatment. IR (-/+): 0/16 Gy X-ray; MCELNs (-/+): 0/10 ug/mL. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35509278/) licensed under a CC-BY license.

MCELNs decreased the DNA damage of H9C2 cells after radiation. (A) Immunofluorescence staining of gamma -H2A.X (green) in H9C2 cells after 48 h of culture with indicated treatment. The nucleus were stained with DAPI (blue), scale bar: 10 μm. Western blot images (B) and quantitation (C) of gamma -H2A.X in H9C2 cells after 48 h of culture with indicated treatment. (D) Immunofluorescence staining of p-ATM (green) in H9C2 cells after 48 h of culture with indicated treatment. The nucleus were stained with DAPI (blue), scale bar: 10 μm. Western blot images (E) and quantitation (F) of p-ATM and ATM in H9C2 cells after 48 h of culture with indicated treatment. IR (–/+): 0/16 Gy X-ray; MCELNs (–/+): 0/10 μg/mL. All data were represented as means +/- SD (n = 3 independent experiments). The statistical significance was evaluated by one-way ANOVA followed by the Turkey's multiple comparisons test among groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35509278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

OOEP is required for ATM activation and RAD51 recruitment to DNA damage sites Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29955025), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca(-)

Species Glossary

Reactivity: Hu, Mu, Rt, Ca(-)

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-306AF350
Alexa Fluor 405	NB100-306AF405
Alexa Fluor 488	NB100-306AF488
Alexa Fluor 532	NB100-306AF532
Alexa Fluor 594	NB100-306AF594
Alexa Fluor 647	NB100-306AF647
Alexa Fluor 700	NB100-306AF700
Alexa Fluor 750	NB100-306AF750
Allophycocyanin	NB100-306APC
Biotin	NB100-306B
CoraFluor 1	NB100-306CL1
DyLight 350	NB100-306UV
DyLight 405	NB100-306V
DyLight 488	NB100-306G
DyLight 550	NB100-306R
DyLight 594	NB100-306DL594
DyLight 650	NB100-306C
DyLight 680	NB100-306FR
DyLight 755	NB100-306IR
FITC	NB100-306F
HRP	NB100-306H
Janelia Fluor 525	NB100-306JF525
Janelia Fluor 549	NB100-306JF549
Janelia Fluor 646	NB100-306JF646
Janelia Fluor 669	NB100-306JF669
PE	NB100-306PE
PerCP	NB100-306PCP
mFluor Violet 450 SE	NB100-306MFV450
mFluor Violet 500 SE	NB100-306MFV500
mFluor Violet 610 SE	NB100-306MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-306SS
Azide and BSA Free	NBP2-80586

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NB110-89719

Ki67/MKI67 Antibody - BSA Free

(3 Reviews)

(28 Publications)

0.1 ml

NB110-89719

<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719] - Ki67/MKI67 Antibody [NB110-89719] - Effect of saireito on the suppression of cell proliferation induced by 5-fluorouracil (5-FU) in mouse small intestines. 5-FU (50 mg/kg) was injected i.p. while saireito (1000 mg/kg) was administered p.o. twice, 30 min before and 8 h after 5-FU injection. The jejunum was excised, sectioned, and Ki67 immunostaining was performed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116213), licensed under a CC-BY license.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Detection of Ki67 in formalin-fixed paraffin embedded mouse intestine using NB110-89719.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Ki-67 (NB110-89719) used at a dilution of 1:250.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Immunohistochemical analysis of mouse spleen.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of Ki67 in mouse cornea. Image courtesy of product review by Bo-Yie Chen.

Effect of saireito on the suppression of cell proliferation induced by 5-fluorouracil (5-FU) in mouse small intestines.5-FU (50 mg/kg) was injected i.p. while saireito (1000 mg/kg) was administered p.o. twice, 30 min before and 8 h after 5-FU injection. The jejunum was excised, sectioned, and Ki67 immunostaining was performed (A). The number of proliferative cells was counted (B). Data are presented as the mean ± SEM of 6 mice. #P < 0.05, versus normal (5-FU-untreated).

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-89719] - The increase in iCM number with SB treatment is not due to increased transgene expression or changes in cell proliferation.Evaluation of gene expression of the transgenes at Day 2 via qPCR is not significantly different between +DMSO (black) control & +SB treatment groups (white, A). Evaluation of overall cell proliferation with Ki67 staining in MEFs with time for +DMSO (black) & +SB treatment groups (grey) (B). A time course evaluation of Troponin T (green) & Ki67 (red) did any dual labeled cells for either the +DMSO (top row) & +SB (bottom row) treated groups (C). Scale bar is 100 µM. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0089678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-89719AF350
Alexa Fluor 405	NB110-89719AF405
Alexa Fluor 488	NB110-89719AF488
Alexa Fluor 532	NB110-89719AF532
Alexa Fluor 594	NB110-89719AF594
Alexa Fluor 647	NB110-89719AF647
Alexa Fluor 700	NB110-89719AF700
Alexa Fluor 750	NB110-89719AF750
Allophycocyanin	NB110-89719APC
Biotin	NB110-89719B
CoraFluor 1	NB110-89719CL1
DyLight 350	NB110-89719UV
DyLight 405	NB110-89719V
DyLight 488	NB110-89719G
DyLight 550	NB110-89719R
DyLight 594	NB110-89719DL594
DyLight 650	NB110-89719C
DyLight 680	NB110-89719FR
DyLight 755	NB110-89719IR
FITC	NB110-89719F
HRP	NB110-89719H
Janelia Fluor 525	NB110-89719JF525
Janelia Fluor 549	NB110-89719JF549
Janelia Fluor 646	NB110-89719JF646
Janelia Fluor 669	NB110-89719JF669
PE	NB110-89719PE
PerCP	NB110-89719PCP
mFluor Violet 450 SE	NB110-89719MFV450
mFluor Violet 500 SE	NB110-89719MFV500
mFluor Violet 610 SE	NB110-89719MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-89719SS

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NBP2-24749

KLF4 Antibody - BSA Free

(2 Reviews)

(26 Publications)

0.1 mg

NBP2-24749

Immunohistochemistry: KLF4 Antibody [NBP2-24749] - IF IHC staining. Representative immunofluorescence immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell markers OCT4 (A-C, green), KLF4 (A-C, red) in normal colon (A), low-grade (B) and high-grade (C) colon adenocarcinoma tissue samples. Cell nuclei were counter-stained with 4', 6'-diamidino-2-phenylindole (A-C, blue). Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221963) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of KLF4 [red] on the cells within the tumor nests (TNs) [thin arrows] and those within the peritumoral stroma [arrowheads]. OCT4 [ green] was expressed on the KLF4+ [green, arrowheads] cells within the peritumoral stroma, and also cells within the peritumoral stroma that expressed KLF4 [ red, thick arrows] not expressing OCT4. c-Myc was expressed by cells within the TNs [green, arrows] and those within the peritumoral stroma [arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed KLF4 [red]. Cell nuclei were counterstained with DAPI [blue]. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license.

Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an anti-rabbit IgG PE conjugated secondary .

Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the (1) absence and (2) presence of immunizing peptide, (3) mouse NIH 3T3, and (4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). Goat anti-rabbit IgG HRP secondary and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative 3,3-diaminobenzidine immunohistochemical stained images of WHO grade I meningioma for OCT4 (A, brown), NANOG (B, purple), SOX2 (C, brown), KLF4 (D, purple) & c-MYC (E, brown) expressed on the endothelial (red arrows) & pericyte (black arrows) layers. Cell nuclei were counterstained with hematoxylin (A-E, blue). Original magnification: 400X. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: KLF4 Antibody [NBP2-24749] - Representative Western blot images of total protein extracted from four head & neck metastatic malignant melanoma-derived primary cell lines probed for induced pluripotent stem cell markers. Blots were probed for OCT4 (A), NANOG (B), SOX2 (C), KLF4 (D), & c-MYC (E) & detected with HRP conjugated goat anti-rabbit antibody. alpha -Tubulin was used as the loading control (F) & detected using HRP conjugated mouse IgGĸ binding protein. Arrows indicate the presence of the proteins with their expected band sizes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32019273), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: KLF4 Antibody [NBP2-24749] - Representative immonoflourescence immunohistochemical stained images of WHO grade I meningioma demonstrating the expression of SOX2 (A, red), NANOG (B, red), KLF4 (C, red), OCT4 (D, green) & c-MYC (E, green) on both the SMA+ (A–C, red, red arrows) pericyte layer, & the ERG+ endothelial layer (D,E, red, white arrows). Cell nuclei were counterstained with 4′, 6′-diamino-2-phenylindole (A–F), blue. Original magnification: 600X. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative colorimetric in situ hybridization stained images of WHO grade I meningioma demonstrating mRNA transcript expression for OCT4 (A, brown), NANOG (B, brown), SOX2 (C, brown), KLF4 (D, brown) & c-MYC (E, brown). Nuclei were counterstained with hematoxylin (A-E, blue). Orignal magnification: 1,000X. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30417000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of SOX2 [(A,D), red], NANOG [(B,E), red], KLF4 [(C,F), red], & c-Myc [(D–F), green] on the cells within the tumor nests (TNs) [(A–F), thin arrows] & those within the peritumoral stroma [(A–F), arrowheads]. OCT4 [(A–C), green] was expressed on the SOX2+ [(A), green, arrowheads], NANOG+ [(B), green, arrowheads], & KLF4+ [(C), green, arrowheads] cells within the peritumoral stroma. There were also cells within the peritumoral stroma that expressed SOX2 [(A), red, thick arrows], NANOG [(B), red, thick arrows] & KLF4 [(C), red, thick arrows] that did not express OCT4. c-Myc was expressed by cells within the TNs [(D–F), green, arrows] & those within the peritumoral stroma [(D–F), arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed SOX2 [(D), red], NANOG [(E), red], & KLF4 [(F), red]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Bv, Pm

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6081A

Alt Catalog# IMG-6081A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24749SS

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NB100-473

Mre11 Antibody (12D7) - Azide and BSA Free

(3 Reviews)

(25 Publications)

100 ul

NB100-473

Western Blot: Mre11 Antibody (12D7) [NB100-473] - Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of human Mre11-transfected 293T cells. 7.5% SDS-PAGE. Mre11 antibody [12D7] (NB100-473). HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Bands are prominent at the theoretical molecular weight of 80 kDa.

Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Analysis of Mre11 antibody (12D7) on lymphoblastoid cells. Dilution 1:200. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Mre11 stained by Mre11 antibody [12D7] (NB100-473).Blue: Hoechst 33342 staining.Scale bar= 10 um.

Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Mouse fibroblasts stained with Mre11 Antibody (12D7). ICC/IF image submitted by a verified customer review.

Western Blot: Mre11 Antibody (12D7) [NB100-473] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (NB100-473) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB200-111

p14ARF/CDKN2A Antibody - BSA Free

(2 Reviews)

(19 Publications)

0.1 ml

NB200-111

Immunocytochemistry/Immunofluorescence: p14ARF/CDKN2A Antibody [NB200-111] - p14ARF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). Tubulin was stained with alpha tubulin (red).

<b>Biological Strategies Validation. </b>Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - ARF expression levels in OCI/AML3 cells after proteasome treatment. This image was submitted via customer Review.

Flow Cytometry: p14ARF/CDKN2A Antibody [NB200-111] - p14ARF antibody was tested at 1:400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - Analysis of HeLa Whole Cell Lysate (NB800-PC1) using p14ARF antibody (lot C) employing ECL detection method.

Immunohistochemistry-Frozen: p14ARF/CDKN2A Antibody [NB200-111] - Mouse brain section, hippocampal (10 um thickness). 3% PFA perfused. 1:1000 dilultion. Image from verified customer review. Image using the Biotin form of this antibody.

Western Blot: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT decreases p53mut stability(A) T24 cells & PSN1 cells were treated with MK-2206 (5 μM) or DMSO for 24 hrs. Cells were fixed & stained with DAPI & anti-mutant p53 (OP29 clone). (B) T24 cells were pre-treated with MK-2206 (5 μM) or DMSO for 24 hrs before the addition of fresh media containing cyclohexamide (100 μM) (CHX) in combination with MK-2206 (5 μM) or DMSO for the times indicated. Nuclear extracts were prepared from treated cells & blotted with the indicated antibodies. Quantification is relative to initiation of CHX treatment for both conditions *, MK-2206 is 40% of DMSO control but taken as 1.0 for relative assessment. (C) H1299 cells were transfected with HA-tagged-ubiquitin & mutant p53 (R175H or R248W) as indicated. Transfected cells were treated with DMSO, MK-2206 (5 μM) or Nutlin3A (5 μM) for 16hrs as indicated. p53 immunoprecipitates & whole cell lysates were probed with the indicated antibodies. (D) T24 cells were transfected with non-targeting control or p14ARF siRNA & treated with DMSO, MK-2206 (5 μM) or the NPM oligomerisation inhibitor NCS348884 (4 μM) (Qi et al., 2008) as indicated. (E) T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 μM), Nutlin3A (5 μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. (F) KPC mice derived KRASG12D p53 Floxed (p53Fl), KRASG12D p53R172H ARF+/+ & KRASG12D p53R172H ARF−/− pancreatic tumor cells were treated with MK-2206 (1μM), Nutlin3A (5μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT modulates p53 stability in-vivo & synergizes with ionizing radiation to inhibit tumor growth(A) PSN1 xenografts (PSN1 cells co-injected with LTC-14 stellate cells) established in the flank of athymic nude mice were treated with MK-2206 (60 mg/kg-320 mg/kg) as indicated or beta -cyclo-dextrin (1.5 mg/ml) carrier. Xenograft tumors were lysed & lysates probed by western blot with the indicated antibodies. (B-D) Sections of PSN1 xenografts treated with three consecutive doses of MK-2206 (60 mg/kg). (B) Sections of PSN1 xenografts & in-vitro PSN1 cells fixed & stained with anti-NPM (red) & anti-p14ARF (green). (C) PSN1 xenografts treated with MK-2206 or carrier were stained with DAPI, anti-p53 (DO1) or p53mut (OP29 clone) (D) PSN1 xenografts treated with MK-2206 (60 mg/kg) or carrier were stained by immunohistochemical methods with anti-pS473-AKT, anti-phospho-S48-NPM (pS48-NPM) or p53. (E) PSN1 xenografts established in the flank of athymic nude mice were injected subcutaneously with two alternate day doses of MK-2206 (60 mg/kg) or carrier. Mice were subsequently treated with a single dose of IR (6 Gy) & tumor volumes measured regularly with callipers. Dash lines indicate tumor growth differential at 250 mm3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT promotes enhanced MDM2 activity via the increased association between NPM & p14ARF(A) Npm−/−, p53−/−double null MEF were infected with pBABE retrovirus empty vector & pBABE expressing FLAG-tagged-NPM-WT, NPM-S48A or S48E as indicated. Immunopurification of NPM was done by pulling down with the Flag tag (middle panel) followed by elution of complexes by the Flag peptide & subsequent immunopurification of endogenous MDM2 (lower panel). (B) Nuclear immunoprecipitates of MDM2 from T24 cells treated with MK-2206 (5 μM, 24 hrs). Immunoprecipitates & lysates were blotted with the indicated antibodies. (C) T24 cells were treated with MK-2206 (5 μM) as indicated. p14ARF was immunoprecipitated from whole cell lysates & nuclear extracts & the association with NPM & MDM2 determined by western blot. Immunoprecipitates & lysates were blotted with the indicated antibodies. (D) MDM2 & (E) p53 ubiquinitation assay in H1299 cells transfected with wild type p53, HA-tagged ubiquitin & treated for 16 hrs with DMSO, MK-2206 (5 μM) or Nutlin3A (5 μM) as indicated. Immunoprecipitates & whole cell lysates were probed with the indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-111AF350
Alexa Fluor 405	NB200-111AF405
Alexa Fluor 488	NB200-111AF488
Alexa Fluor 532	NB200-111AF532
Alexa Fluor 594	NB200-111AF594
Alexa Fluor 647	NB200-111AF647
Alexa Fluor 700	NB200-111AF700
Alexa Fluor 750	NB200-111AF750
Allophycocyanin	NB200-111APC
Biotin	NB200-111B
CoraFluor 1	NB200-111CL1
DyLight 350	NB200-111UV
DyLight 405	NB200-111V
DyLight 488	NB200-111G
DyLight 550	NB200-111R
DyLight 594	NB200-111DL594
DyLight 650	NB200-111C
DyLight 680	NB200-111FR
DyLight 755	NB200-111IR
FITC	NB200-111F
HRP	NB200-111H
Janelia Fluor 525	NB200-111JF525
Janelia Fluor 549	NB200-111JF549
Janelia Fluor 646	NB200-111JF646
Janelia Fluor 669	NB200-111JF669
PE	NB200-111PE
PerCP	NB200-111PCP
mFluor Violet 450 SE	NB200-111MFV450
mFluor Violet 500 SE	NB200-111MFV500
mFluor Violet 610 SE	NB200-111MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB200-111SS

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NBP2-29463

p21/CIP1/CDKN1A Antibody (WA-1)

(26 Publications)

0.1 mg

NBP2-29463

p66Shc-/- induces premature cell cycle arrest during aging. (A-B) p21 protein expression in lung homogenate at different ages in WT and p66Shc-/- mice. n=4-5. (C-E) p21 protein expression in lung homogenates from WT and p66Shc-/- mice of corresponding ages. n=6. (F-H) Lamin B1 protein expression in lung homogenates from WT and p66Shc-/- mice of corresponding ages. The expression of p21 and lamin B1 is depicted as a ratio relative to beta -actin expression. (I) Senescence associated beta -galactosidase (SA beta -gal) activity in fresh cryo-sections from lungs of WT and p66Shc-/- mice at 3, 12, and 24 months of age (n= 3-4 mice per group). Thirty parenchymal fields were randomly selected for each lung to quantify the number of beta - galactosidase positive cells. (J-L) mRNA expression of (J) IL6, (K) IL-1 beta beta, and (L) TNF alpha in lung homogenates of p66Shc-/- and WT during aging. n=3 mice per group. Data were log-transformed for analysis. (A-B) Tests were performed as general linear hypothesis tests and P-values were adjusted for multiple testing using the

Aldehyde 13-13 acts to reduce cellular senescence: (A) OR2H2 expression comparison between non-senescent (Non-S) and senescent (S) cells via qPCR; (B) Immunocytochemical depiction of Ki-67 (green) in senescent cells exposed to aldehyde 13-13 (40 uM). DAPI is shown in red; (C) Immunoblot assessment of P16 and P21 expression in senescent cells treated with aldehyde 13-13; (D) Examination of morphology and expression of senescence-associated beta -galactosidase (SA beta -Gal) in VK2/E6E7 cells. Tests were performed in three or four replicates. Ctrl, control; Ald 13-13, aldehyde 13-13. Data are presented as mean +/- SD. Two-tailed unpaired t-test was applied for multiple or two-group comparisons. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37765029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Pm, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

ABX-129A

Alt Catalog# ABX-129A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33084AF350
Alexa Fluor 405	NBP2-33084AF405
Alexa Fluor 488	NBP2-33084AF488
Alexa Fluor 532	NBP2-33084AF532
Alexa Fluor 594	NBP2-33084AF594
Alexa Fluor 647	NBP2-33084AF647
Alexa Fluor 700	NBP2-33084AF700
Alexa Fluor 750	NBP2-33084AF750
Allophycocyanin	NBP2-33084APC
Biotin	NBP2-33084B
CoraFluor 1	NBP2-33084CL1
DyLight 350	NBP2-33084UV
DyLight 405	NBP2-33084V
DyLight 488	NBP2-33084G
DyLight 550	NBP2-33084R
DyLight 594	NBP2-33084DL594
DyLight 650	NBP2-33084C
DyLight 680	NBP2-33084FR
DyLight 755	NBP2-33084IR
FITC	NBP2-33084F
HRP	NBP2-33084H
Janelia Fluor 525	NBP2-33084JF525
Janelia Fluor 549	NBP2-33084JF549
Janelia Fluor 646	NBP2-33084JF646
Janelia Fluor 669	NBP2-33084JF669
PE	NBP2-33084PE
PerCP	NBP2-33084PCP
mFluor Violet 450 SE	NBP2-33084MFV450
mFluor Violet 500 SE	NBP2-33084MFV500
mFluor Violet 610 SE	NBP2-33084MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33084
IHC-Prediluted	NBP2-44524

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NB110-90592

Ki67/MKI67 Antibody - BSA Free

(1 Review)

(18 Publications)

0.1 ml

NB110-90592

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Deregulated MYC transforms breast acini into invasive breast carcinoma in vivo. Tumours harvested in Figure 1C were stained (above) for Ki67/MKI67 and TUNEL and were quantified (below) for the degree of Ki67/MKI67 and TUNEL staining. Individual quantifications per tumour are shown; *P<=0.05, ***P<=0.001, one-way ANOVA with Bonferroni post-test for multiple testing. Scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.038083), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Ki-67/MKI67 (NB110-90592) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (20X)

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (40X)

Ki67/MKI67 was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-Ki67/MKI67 Affinity Purified Polyclonal Antibody conjugated to Biotin (Catalog # NB110-90592B) at 2 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Ze

Species Glossary

Reactivity: Hu, Mu, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-90592AF350
Alexa Fluor 532	NB110-90592AF532
Alexa Fluor 594	NB110-90592AF594
Alexa Fluor 750	NB110-90592AF750
Allophycocyanin	NB110-90592APC-0.1ml
Biotin	NB110-90592B
CoraFluor 1	NB110-90592CL1
DyLight 350	NB110-90592UV-0.1ml
DyLight 405	NB110-90592V-0.1ml
DyLight 488	NB110-90592G
DyLight 550	NB110-90592R
DyLight 594	NB110-90592DL594
DyLight 650	NB110-90592C
DyLight 680	NB110-90592FR-0.1ml
DyLight 755	NB110-90592IR-0.1ml
FITC	NB110-90592F-0.1ml
HRP	NB110-90592H
Janelia Fluor 525	NB110-90592JF525
Janelia Fluor 549	NB110-90592JF549
Janelia Fluor 646	NB110-90592JF646
Janelia Fluor 669	NB110-90592JF669
PE	NB110-90592PE-0.1ml
PerCP	NB110-90592PCP-0.1ml
mFluor Violet 450 SE	NB110-90592MFV450
mFluor Violet 500 SE	NB110-90592MFV500
mFluor Violet 610 SE	NB110-90592MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-90592SS

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NB100-56340

HDAC1 Antibody - BSA Free

(2 Reviews)

(19 Publications)

0.1 mg

NB100-56340

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-337

Alt Catalog# IMG-337

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56340SS

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NB200-108

c-Myc Antibody (9E11) - BSA Free

(3 Reviews)

(12 Publications)

0.1 ml

NB200-108

Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U937 cells with c-Myc [9E11] Antibody NB200-108AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: c-Myc Antibody (9E11) [NB200-108] - Whole cell protein from PC3 (lane 1), U-2 OS (lane 2) and mouse testis (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-c-Myc in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers in low SVCT-2 expressing cell lines. Expression of c-Myc and cyclin D1 was analyzed by western blotting in HCT15 and DLD-1 cell lines after L-ascorbic acid treatment. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.

Flow (Intracellular): c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E11) NB200-108AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E11) NB200-108PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.

Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody [9E11] NB200-108 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody.

Reactivity:

Hu, Mu, Rt, Ch, Ye

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Ye

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-108AF350
Alexa Fluor 405	NB200-108AF405
Alexa Fluor 488	NB200-108AF488
Alexa Fluor 532	NB200-108AF532
Alexa Fluor 594	NB200-108AF594
Alexa Fluor 647	NB200-108AF647
Alexa Fluor 700	NB200-108AF700
Alexa Fluor 750	NB200-108AF750
Allophycocyanin	NB200-108APC
Biotin	NB200-108B
CoraFluor 1	NB200-108CL1
DyLight 350	NB200-108UV
DyLight 405	NB200-108V
DyLight 488	NB200-108G
DyLight 550	NB200-108R
DyLight 594	NB200-108DL594
DyLight 650	NB200-108C
DyLight 680	NB200-108FR
DyLight 755	NB200-108IR
FITC	NB200-108F
HRP	NB200-108H
Janelia Fluor 525	NB200-108JF525
Janelia Fluor 549	NB200-108JF549
Janelia Fluor 646	NB200-108JF646
Janelia Fluor 669	NB200-108JF669
PE	NB200-108PE
PerCP	NB200-108PCP
mFluor Violet 450 SE	NB200-108MFV450
mFluor Violet 500 SE	NB200-108MFV500
mFluor Violet 610 SE	NB200-108MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB200-108SS
Azide and BSA Free	NBP2-80674

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NB100-56694

TRF-2 Antibody - BSA Free

(13 Publications)

0.1 mg

NB100-56694

<b>Genetic Strategies Validation. </b>Western Blot: TRF-2 Antibody [NB100-56694] - U2OS cells were transduced with a lentiviral vector coding for a Dox inducible control shRNA (shCtrl) or a shRNA against TRF-2 (shTRF2) and selected with puromycin +/- Dox for a week. A) Western blot analysis of TRF-2 expression one week post selection. Membranes were also probed with anti-tubulin antibodies to show the input material loaded. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442/) licensed under a CC-BY license.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - U2OS cells were transduced with a lentiviral vector coding for a Dox inducible control shRNA (shCtrl) or a shRNA against TRF-2 (shTRF2) and selected with puromycin +/- Dox for a week. One week post selection, +Dox cells were infected with HHV-6A for 48h and processed for IFA using anti-TRF-2 (green) and anti-IE2 (red). Cells with IE2 in punctate form and cells with large patchy IE2, likely to represent VRC, are shown. Nuclei are outlined by dashed lines. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442/) licensed under a CC-BY license.

Western Blot: TRF-2 Antibody [NB100-56694] - TRF2 knockdown affect HHV-6A/B chromosomal integration.A) U2OS cells were transduced with lentiviral vectors expressing a scrambles shRNA (shCtrl) or a shTRF2. After a week of selection, cells were monitored for TRF2 expression by western blot. B) After a week of selection, shCtrl & shTRF2 treated cells were infected with HHV-6A or HHV-6B. After 30 days, DNA was isolated & the relative frequency of integration, relative to shCtrl set at 100%, estimated by ddPCR. *p<0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - TRF2 expression in HHV-6A-infected U2OS cells.U2OS cells were infected with HHV-6A & analyzed for TRF2 & IE2 expression at 24h, 48h & 72h post-infection by dual color immunofluorescence. A) Representative immunofluorescence of TRF2 & IE2 expression in bystander & IE2 expressing cells at 24, 48h & 72h post infection. B) Mean relative TRF2 expression ± SD in uninfected (blue), IE2- (green-uninfected bystander) or IE2+ (red-infected) cells at 24h, 48h & 72h post infection. Each symbol represents the relative TRF2 expression from a single nucleus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - Binding of TRF2 to viral DNA during HHV-6A/B infection.A) Schematic representation of the HHV-6A/B genome. The DR6 probe used for hybridization is shown in red. Uninfected & HHV-6A-infected HSB-2 cells (B-D) or uninfected & HHV-6B-infectd Molt-3 cells (E-F) were analyzed for TRF2 binding to viral DNA using ChIP. The input was hybridized with Alu probe to assess quantity of starting material. Anti-IgG (negative control), anti-PolII (positive control) or TRF2 antibodies were used for immunoprecipitation. B) QPCR detection of GAPDH DNA following ChIP. Results are expressed as fold increase over control IgG. C & E) Eluted DNA was hybridized with 32P-labeled Alu, telomeric (TTAGGG)3 or HHV-6A (DR6) probes. After hybridization the membranes were washed & exposed to X-ray films. D & F) Densitometric analysis of relative binding of TRF2 to telomeric & viral DNA. Results of one experiment representative of three are presented & are expressed as signal after normalization to input. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - TRF2 is required for IE2 localization with telomeres.U2OS cells were transduced with a lentiviral vector coding for a Dox inducible control shRNA (shCtrl) or a shRNA against TRF2 (shTRF2) & selected with puromycin +/- Dox for a week. A) Western blot analysis of TRF2 expression one week post selection. Membranes were also probed with anti-tubulin antibodies to show the input material loaded. B) One week post selection, +Dox cells were infected with HHV-6A for 48h & processed for IFA using anti-TRF2 (green) & anti-IE2 (red). Cells with IE2 in punctate form & cells with large patchy IE2, likely to represent VRC, are shown. Nuclei are outlined by dashed lines. C) The percentage of HHV-6A infected cells (from B) was estimated after counting a minimum of 700 cells & scoring the IE2+ ones. Results are expressed as mean %IE2+ cells ± SD. D) Mean percentage ± SD of IE2 localizing with telomeres in the presence (shCtrl +Dox) & absence (shTRF2 +Dox) of TRF2. Each dot represents the % of IE2 foci localizing with telomeres in one nucleus. ****p<0.0001. E) IF-FISH confocal images of shCtrl (+Dox) & shTRF2 (+Dox) cells analyzed for TRF2 (green), IE2 (red) & telomeres (cyan). Nuclei are outlined by dashed circles. Examples of IE2 localizing with telomeres (top row) or not found with telomeres (bottom row) are highlighted by the dashed polygons. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - A) A stick diagram of the IE2 protein with various domains identified is presented. B) Colocalization of HHV-6A IE2 protein with telomeres in the absence of viral DNA. U2OS cells were transfected with an empty vector, with IE2 expression vector or with IE2 delta 1290–1500 expression vector. Forty-eight hours later cells were processed for dual color immunofluorescence. Telomeres were labeled in cyan & IE2 in red. Nuclei are outlined by dashed lines. Examples of IE2 colocalizing with telomeres are presented in a 3D view (white arrows). C) The graph represents the mean ± SD % of WT IE2 & delta 1290–1500 IE2 localizing with telomeres. D) Lack of colocalization between HHV-6A p41 & telomeres in uninfected cells. U2OS cells were transfected with an empty vector or with a p41 expression vector. Forty-eight hours later cells were processed for dual color immunofluorescence. TRF2 was labeled green, p41 in red & nuclei outlined by a dashed line. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - Colocalization of shelterin complex proteins & HHV-6A IE2 protein at VRC & cellular telomeres.A) U2OS cells were mock-infected or infected with HHV-6A for 48h after which cells were processed for IF-FISH. Telomeres were labeled in magenta, TRF2 in green & IE2 in red. The panels in the middle row show images of cells with IE2 patches overlapping with large, diffuse TRF2 & telomeric staining (rectangles). The panels in the third row represent infected cells with punctate IE2 pattern colocalizing with TRF2 & telomeres (dashed squares). The colocalization of IE2, TRF2 & telomeres are shown in both 2D & 3D images. B) Uninfected & HHV-6A-infected U2OS cells were transfected with an empty vector, a myc-tagged-TRF1 expression vector. Forty-eight hours later cells were processed for IF-FISH. TRF1 was labeled in green & IE2 in red. Nuclei were stained with DAPI. Images on the far right show 2D colocalization of TRF1 with IE2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-148A

Alt Catalog# IMG-148A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56694SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

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Marker

Modification

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Clone

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Species Glossary

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