| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96140SS | |||
| Azide and BSA Free | NBP2-80599 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44995SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24669SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87321-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40823SS | |||
![Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-783-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)")
![Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783] - Staining of paraffin embedded Human Lymph Node. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunohistochemistry-Paraffin-NB100-783-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0008.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0009.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0003.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on NIH3T3 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0012.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0005.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0007.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U937 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0011.jpg)
![Western Blot: Folliculin Antibody [NBP1-44995] - Analysis of FLCN isoforms 1, 2 and 3 on HeLa whole cell lysate](http://images.novusbio.com/fullsize/Folliculin-Antibody-Western-Blot-NBP1-44995-img0004.jpg "Western Blot: Folliculin Antibody [NBP1-44995] - Analysis of FLCN isoforms 1, 2 and 3 on HeLa whole cell lysate")
![Immunocytochemistry/Immunofluorescence: Folliculin Antibody [NBP1-44995] - Detection of FLCN (Green) in Hela cells using NBP1-44995. Nuclei (Blue) are counterstained using Hoechst 33258](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44995-img0002.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. The SCC cells as well as the associated stromal cells depicted strong cytoplasmic-nuclear immunostaining of Folliculin protein. The staining was more intense in the carcinoma cells compared to the tumor stroma [Magnification 10X]](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0014.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. The carcinoma cells as well as the various stromal cells depicted cytoplasmic- nuclear immunostaining for Folliculin protein. The cytoplasmic staining was more intense in the carcinoma cells compared to tumor stroma [Magnification 40X]](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0010.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Cytoplasmic-nuclear immunostaining of Folliculin protein was observed in the various cells and interestingly, the staining was intense and localized more towards the cell membranes in a bunch of cells having mesenchymal morphology. [Magnification 40X]](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0012.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal skin from human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Intense cytoplasmic with moderate nuclear immunostaining of Folliculin protein was observed in the various cells of the epidermal layer [Magnification 40X].](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0013.jpg)
![Immunohistochemistry-Paraffin: Folliculin Antibody [NBP1-44995] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal liver of human using rabbit polyclonal Folliculin antibody (NBP1-44995) at 5 ug/ml concentration. Strong cytoplasmic-nuclear immunopositivity of Folliculin protein was observed in hepatocytes [Magnification 10X].](http://images.novusbio.com/fullsize/Folliculin-Antibody-Immunohistochemistry-Paraffin-NBP1-44995-img0009.jpg)
![Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunoprecipitation-NBP1-41201-img0017.jpg "Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-western-blot-196202494114..jpg)
![RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] (NBP1-41201) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-immunocytochemistry-immunofluorescence-2611202518283514.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DLL3-Antibody-Western-Blot-NBP2-24669-img0001.jpg "Western Blot: DLL3 Antibody [NBP2-24669] - Analysis of DLL3 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) T98G cell lysate using DLL3 antibody at 3 and 5 ug/mL respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: DLL3 Antibody [NBP2-24669] - Human brain tissue probed with an isotype control (top) and DLL3 antibody (bottom) at 5 ug/mL.](http://images.novusbio.com/fullsize/DLL3-Antibody-Immunohistochemistry-Paraffin-NBP2-24669-img0002.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0007.jpg "Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunocytochemistry-Immunofluorescence-NBP2-22204-img0002.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Flow-Cytometry-NBP2-22204-img0001.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0006.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0003.jpg)
![ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-ELISA-NBP2-22204-img0005.jpg)
![Western Blot: CTBP2 Antibody (1R1U6) [NBP3-15268] - Analysis of extracts of various cell lines, using CTBP2 antibody (NBP3-15268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CTBP2-Antibody-1R1U6-Western-Blot-NBP3-15268-img0007.jpg "Western Blot: CTBP2 Antibody (1R1U6) [NBP3-15268] - Analysis of extracts of various cell lines, using CTBP2 antibody (NBP3-15268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CTBP2 Antibody (1R1U6) [NBP3-15268] - Rat ovary using CTBP2 Rabbit mAb (NBP3-15268) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CTBP2-Antibody-1R1U6-Immunohistochemistry-Paraffin-NBP3-15268-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CTBP2 Antibody (1R1U6) [NBP3-15268] - Immunofluorescence analysis of NIH/3T3 cells using CTBP2 Rabbit mAb (NBP3-15268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CTBP2-Antibody-1R1U6-Immunocytochemistry-Immunofluorescence-NBP3-15268-img0004.jpg)
![Immunohistochemistry-Paraffin: CTBP2 Antibody (1R1U6) [NBP3-15268] - Human colon carcinoma using CTBP2 Rabbit mAb (NBP3-15268) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CTBP2-Antibody-1R1U6-Immunohistochemistry-Paraffin-NBP3-15268-img0001.jpg)
![Immunohistochemistry-Paraffin: CTBP2 Antibody (1R1U6) [NBP3-15268] - Mouse kidney using CTBP2 Rabbit mAb (NBP3-15268) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CTBP2-Antibody-1R1U6-Immunohistochemistry-Paraffin-NBP3-15268-img0002.jpg)
![Immunoprecipitation: CTBP2 Antibody (1R1U6) [NBP3-15268] - Analysis of 300ug extracts of Hela cells using 3ug CTBP2 antibody (NBP3-15268). Western blot was performed from the immunoprecipitate using CTBP2 (NBP3-15268) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/CTBP2-Antibody-1R1U6-Immunoprecipitation-NBP3-15268-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody (1R1U6) [CTBP2] - Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15268_rabbit-ctbp2-mab-1r1u6-5220251726010.jpg)
![Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody (1R1U6) [CTBP2] - Immunofluorescence analysis of PC-12 cells using CTBP2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15268_rabbit-ctbp2-mab-1r1u6-52202517192921.jpg)
![Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody (1R1U6) [CTBP2] - Immunofluorescence analysis of NIH/3T3 cells using CTBP2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15268_rabbit-ctbp2-mab-1r1u6-5220251721359.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.Positive control:Lane 1: NIH-3T3 Lane 2: HelaLane 3: Rat brain Lane 4: Mouse testis](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Western-Blot-NBP2-75642-img0010.jpg "Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.Positive control:Lane 1: NIH-3T3 Lane 2: HelaLane 3: Rat brain Lane 4: Mouse testis")
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunocytochemistry-Immunofluorescence-NBP2-75642-img0004.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0009.jpg)
![Flow Cytometry: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Flow-Cytometry-NBP2-75642-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunocytochemistry-Immunofluorescence-NBP2-75642-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunocytochemistry-Immunofluorescence-NBP2-75642-img0003.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0005.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0006.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0007.jpg)
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-JU37-47-Immunohistochemistry-Paraffin-NBP2-75642-img0008.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0012.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0007.jpg)
![Western Blot: BMI-1 Antibody [NBP1-87321] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-87321-img0008.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0010.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human prostate shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0011.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-87321] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0013.jpg)
![Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP2-24693-img0006.jpg "Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-24693] - Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and neck SCC) lines established by the Gollin lab (White et al., 2007). The BMI1 antibody was used at 5 ug/ml. Staining is primarily nuclear, some cytoplasmic staining is also observed. Data courtesy of Hatem Kaseb, MD and Susanne M. Gollin, PhD.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24693-img0004.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24693-img0005.jpg)
![Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle](http://images.novusbio.com/fullsize/Bmi1-Antibody-Western-Blot-NB110-40823-img0002.jpg "Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle")
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Bmi1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40823-img0004.jpg)