| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-353SS | |||
| Azide and BSA Free | NBP2-80606 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88517-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70314 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47264-25ul | |||
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0016.jpg "Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024205614.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-91020242024619.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024200109.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-910202420134060.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-western-blot-1411202518334521.jpg)
![Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-NB100-563-img0006.jpg "Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NB100-563-img0007.jpg)
![Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Western-Blot-NB100-353-img0006.jpg "Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Immunocytochemistry-Immunofluorescence-NB100-353-img0005.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0008.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0007.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0011.jpg "Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.")
![Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32775-img0013.jpg)
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-Paraffin-NBP1-32775-img0004.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0005.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat 2 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0007.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0009.jpg)
![Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP1-32775] - Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 ug of MAD1 antibody. Western blot analysis was performed using MAD1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NBP1-32775-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88517-img0008.jpg "Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0014.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0001.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0011.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0012.jpg)
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-88517-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0021.jpg "Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-31311] - Analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31311-img0008.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0019.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - DLD-1 xenograft, using Mad2L1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0009.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0012.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NBP1-31311-img0017.jpg)
![Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Western-Blot-NBP1-47680-img0002.jpg "Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680] - BubR1 Antibody (6E5) [NBP1-47680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunocytochemistry-Immunofluorescence-NBP1-47680-img0012.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0009.jpg)
![Flow Cytometry: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Flow-Cytometry-NBP1-47680-img0011.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0003.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0004.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0005.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0006.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0007.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0008.jpg)
![Western Blot: Bub3 Antibody (10G1S6) [NBP3-16709] - Western blot analysis of extracts of various cell lines, using Bub3 Rabbit mAb (NBP3-16709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Bub3-Antibody-10G1S6-Western-Blot-NBP3-16709-img0006.jpg "Western Blot: Bub3 Antibody (10G1S6) [NBP3-16709] - Western blot analysis of extracts of various cell lines, using Bub3 Rabbit mAb (NBP3-16709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunofluorescence analysis of U-2 OS cells using Bub3 Rabbit mAb (NBP3-16709) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Bub3-Antibody-10G1S6-Immunocytochemistry-Immunofluorescence-NBP3-16709-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunofluorescence analysis of C6 cells using Bub3 Rabbit mAb (NBP3-16709) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Bub3-Antibody-10G1S6-Immunocytochemistry-Immunofluorescence-NBP3-16709-img0004.jpg)
![Immunohistochemistry: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry analysis of Bub3 in paraffin-embedded rat brain tissue using Bub3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16709_rabbit-bub3-mab-10g1s6-8220252010426.jpg)
![Immunohistochemistry: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry analysis of Bub3 in paraffin-embedded human colon carcinoma tissue using Bub3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16709_rabbit-bub3-mab-10g1s6-822025209867.jpg)
![Immunohistochemistry: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry analysis of Bub3 in paraffin-embedded human colon tissue using Bub3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16709_rabbit-bub3-mab-10g1s6-822025208436.jpg)
![Immunohistochemistry: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry analysis of Bub3 in paraffin-embedded mouse brain tissue using Bub3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16709_rabbit-bub3-mab-10g1s6-82202520844.jpg)
![Immunohistochemistry: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry analysis of Bub3 in paraffin-embedded rat spleen tissue using Bub3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16709_rabbit-bub3-mab-10g1s6-822025208414.jpg)
![Immunohistochemistry: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry analysis of Bub3 in paraffin-embedded mouse lung tissue using Bub3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16709_rabbit-bub3-mab-10g1s6-822025208455.jpg)
![Immunohistochemistry: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry analysis of Bub3 in paraffin-embedded rat colon tissue using Bub3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16709_rabbit-bub3-mab-10g1s6-8220252010425.jpg)
![Western Blot: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Western-Blot-NBP2-70314-img0001.jpg "Western Blot: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70314-img0003.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0009.jpg)
![Flow Cytometry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70314-img0002.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0004.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0005.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0006.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0007.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0008.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0010.jpg)
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0012.jpg "Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Immunohistochemistry-Paraffin-H00000701-M02-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi ( Cat # H00000701-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody (M02) clone 2G5 (Cat # H00000701-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0009.jpg)
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - BUB1B monoclonal antibody (M02), clone 2G5 Analysis of BUB1B expression in Hela S3 NE.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0011.jpg)
![Proximity Ligation Assay: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Proximity-Ligation-Assay-H00000701-M02-img0006.jpg)
![Sandwich ELISA: BubR1 Antibody (2G5) [H00000701-M02] - Detection limit for recombinant GST tagged BUB1B is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Sandwich-ELISA-H00000701-M02-img0010.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)
![Western Blot: SPC24 Antibody [NBP2-47264] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/SPC24-Antibody-Western-Blot-NBP2-47264-img0003.jpg "Western Blot: SPC24 Antibody [NBP2-47264] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.")
![Immunocytochemistry/Immunofluorescence: SPC24 Antibody [NBP2-47264] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/SPC24-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47264-img0006.jpg)
