Cell Biology reagents: Novus Biologicals

NBP2-57308

MCT8/SLC16A2 Antibody - BSA Free

(6 Publications)

100 ul

NBP2-57308

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of mouse embryo E11 shows strong membranous positivity in the developing circumventricular organ.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human cerebral cortex shows moderate positivity in neuropil.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: MCT8/SLC16A2 Antibody [NBP2-57308] - Staining of human liver shows moderate to strong membranous positivity in hepatocytes.

Expression of MCT8 and OATP1C1 in the cerebral barriers of the human and macaque motor cortex. Representative brightfield (A,E,I,M) and fluorescence confocal microscope (B–D,F–H,J–L,N–T) photomicrographs showing immunostaining for MCT8 (A–H,Q–T) and OATP1C1 (I–P) in blood vessels of human and macaque motor cortex. (A,E) show strong MCT8 staining in the endothelial layer of small vessels and capillaries (white arrowheads) in humans and macaques, respectively. (B–D,F–H) show colocalization of MCT8 (green) with the endothelial markers UEA-I (red) and ENG (red). (I,M) show weak OATP1C1 staining in small vessels (white arrowheads) and venules (black arrowheads). The red arrowhead in M points to a glial cell positive for OATP1C1 with processes surrounding the capillary. (J–L,N–P) show colocalization of OATP1C1 (green) with UEA-I (red) and endoglin (red) in a few blood vessels of the human and macaque motor cortex, respectively. White arrowheads point to vessels. In both human and macaque brain tissues, MCT8-immunopositive capillaries are much more abundant than OATP1C1-immunopositive capillaries, which can only be seen occasionally. Moreover, (Q–T) show MCT8-expressing pericytes (white arrowheads) in the human motor cortex that in addition to express MCT8 (green), also show the pericyte biomarker PDGFR-beta (pink), but not the endothelial marker UEA-I (red). Counterstaining with DAPI (blue) shows nuclei of all cells. ENG: endoglin, UEA-I: Ulex Europaeus Agglutinin-I. PDGFR-beta : platelet-derived growth factor receptor beta. Scale bar = 100 μm (A,E,I,M), 25 μm (B–D), 50 μm (J–L), 120 μm (F–H,N–P), and 12 μm (Q–T). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of MCT8 in pyramidal neurons of the human and macaque motor cortex. Representative brightfield photomicrographs show immunostaining for MCT8 in layers III (A), V (B) and VI (C) of the human motor cortex, and layers II (G), III (H), and V (I) of the macaque motor cortex. White arrowheads point to pyramidal neurons with immunopositive signal in the soma, apical and basal dendrites. Black arrowheads point to smaller cells with the morphology of interneurons. (D–F) (human) and (J–L) (macaque) show confocal microscope images from double-stained sections for MCT8 (green) and the pyramidal neuron markers SMI-32 (red) for 200 kDa neurofilament protein or neurogranin/RC3 (red), respectively. White arrowheads point to pyramidal neurons. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans, the MCT8 signal is located mainly in the pyramidal cell membrane, while in macaques it is located in the membrane and the cytoplasm. Scale bar = 50 μm (A), 120 μm (B,I), 25 μm (C–F), 60 μm (G), 62.5 μm (H), and 43.5 μm (J–L). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of MCT8 and OATP1C1 in human and macaque motor cortex astrocytes. Representative brightfield (A–E,I) photomicrographs of sections immunostained for MCT8 (A,C) and OATP1C1 (B,D,E,I) in cortical layer I (A–D) and subjacent white matter (E,I). Confocal microscope images of human (F–H) and macaque (J–O) sections double-labeled for OATP1C1 (green) and GFAP (red). Counterstaining with DAPI (blue) shows nuclei of all cells. Note that there is full colocalization of both markers. White and red arrowheads point to astrocytes. Blue arrowheads point to a blood vessel. GFAP: glial fibrillary acidic protein. Scale bar = 50 μm (A–D,J–L), 110 μm (E,I), 25 μm (F–H), and 78 μm (M–O). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36834621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-57308-25ul

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NBP1-85814

Vimentin Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-85814

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85814-25ul

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NBP1-89370

S100A10 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-89370

Staining of human lung shows strong membranous positivity in macrophages.

<b>Genetic Strategies Validation. </b>Analysis in human lung and skeletal muscle tissues using NBP1-89370 antibody. Corresponding S100A10 RNA-seq data are presented for the same tissues.

Staining of human small intestine shows strong membranous positivity in glandular cells.

Staining of human skin shows strong membranous positivity in squamous epithelial cells.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines Caco-2 and HEK293 using Anti-S100A10 antibody. Corresponding S100A10 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

<b>Genetic Strategies Validation. </b>Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-S100A10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

The AT2-blocker PD123319 induces S100A10 expression in astrocytes with increased proliferation whereas Telmisartan does not affect S100A10 or Ki67 expression. *p < 0.05, **p < 0.01, and ***p < 0.001 compared different experimental groups as marked by horizontal bar; graphs depict mean values +/- standard error of the mean (SEM). A Experimental timeline. Three to five days after subculturing, astrocytes were left unstimulated or treated with 10 uM telmisartan or 10 uM PD123319 for 48 h. They were then exposed to 2 h of oxygen–glucose deprivation (OGD) before they were allowed to recover in regular culture medium for 24 h. Afterward, the cells were used for further experiments. Unstimulated astrocytes, which were exposed to 2 h of OGD, served as controls. B S100A10 marker expression in untreated (unstimulated) or OGD-treated (2 h of OGD) astrocytes with or without the additional preincubation with 10 uM telmisartan or 10 uM PD123319. C Proliferation rate of untreated (unstimulated) and OGD-treated (2 h OGD) astrocytes, with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h as assessed by mRNA-ki67 expression. D ATP concentration of untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h. E Expression of the connexin Cx43 in untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan and 10 uM PD123319 over 48 h. F Representative immunocytochemical stainings of astrocytes with Glia Fibrillary Acidic Protein (GFAP) + (green) and S100A10 + (red) in unstimulated (control), PD123319-preincubated (PD123319), or after 2 h of OGD (OGD + Control). Hoechst stained all cell nuclei blue; scale bars = 50 um Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38926677), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Rt, Mu

Species Glossary

Reactivity: Hu, Rt, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89370-25ul

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NBP2-02577

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73605AF350
Alexa Fluor 405	NBP2-73605AF405
Alexa Fluor 488	NBP2-73605AF488
Alexa Fluor 532	NBP2-73605AF532
Alexa Fluor 594	NBP2-73605AF594
Alexa Fluor 647	NBP2-73605AF647
Alexa Fluor 700	NBP2-73605AF700
Alexa Fluor 750	NBP2-73605AF750
Allophycocyanin	NBP2-73605APC
Biotin	NBP2-73605B
CoraFluor 1	NBP2-73605CL1
DyLight 350	NBP2-73605UV
DyLight 405	NBP2-73605V
DyLight 488	NBP2-73605G
DyLight 550	NBP2-73605R
DyLight 594	NBP2-73605DL594
DyLight 650	NBP2-73605C
DyLight 680	NBP2-73605FR
DyLight 755	NBP2-73605IR
FITC	NBP2-73605F
HRP	NBP2-73605H
Janelia Fluor 525	NBP2-73605JF525
Janelia Fluor 549	NBP2-73605JF549
Janelia Fluor 646	NBP2-73605JF646
Janelia Fluor 669	NBP2-73605JF669
PE	NBP2-73605PE
PerCP	NBP2-73605PCP
mFluor Violet 450 SE	NBP2-73605MFV450
mFluor Violet 500 SE	NBP2-73605MFV500
mFluor Violet 610 SE	NBP2-73605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73605

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NBP1-97712

Cytokeratin 19 Antibody (RCK108) - BSA Free

(3 Publications)

0.1 mg

NBP1-97712

Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [DyLight 650] [NBP1-97712C] - An intracellular stain was performed on HT-29 cells with Cytokeratin 19 (RCK108) NBP1-97712C (blue) and a matched isotype control NBP1-97005C (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight650. Image using the DyLight 650 form of this antibody.

Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Analysis using the Unpurified version of NBP1-97712. Immunohistochemistry on frozen section of human liver bile duct epithelium

Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on a frozen tissue sections of human kidney with NBP1-97712 (RCK108; dilution 1:500).

Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on frozen tissue sections of human colon with NBP1-97712 (RCK108; dilution 1:500).

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human small intestine paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human placenta paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.

Reactivity:

Hu, Rt, Ze

Species Glossary

Reactivity: Hu, Rt, Ze

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97712AF350
Alexa Fluor 405	NBP1-97712AF405
Alexa Fluor 488	NBP1-97712AF488
Alexa Fluor 532	NBP1-97712AF532
Alexa Fluor 594	NBP1-97712AF594
Alexa Fluor 647	NBP1-97712AF647
Alexa Fluor 700	NBP1-97712AF700
Alexa Fluor 750	NBP1-97712AF750
Allophycocyanin	NBP1-97712APC
Biotin	NBP1-97712B
CoraFluor 1	NBP1-97712CL1
DyLight 350	NBP1-97712UV
DyLight 405	NBP1-97712V
DyLight 488	NBP1-97712G
DyLight 550	NBP1-97712R
DyLight 594	NBP1-97712DL594
DyLight 650	NBP1-97712C
DyLight 680	NBP1-97712FR
DyLight 755	NBP1-97712IR
FITC	NBP1-97712F
HRP	NBP1-97712H
Janelia Fluor 525	NBP1-97712JF525
Janelia Fluor 549	NBP1-97712JF549
Janelia Fluor 646	NBP1-97712JF646
Janelia Fluor 669	NBP1-97712JF669
PE	NBP1-97712PE
PerCP	NBP1-97712PCP
mFluor Violet 450 SE	NBP1-97712MFV450
mFluor Violet 500 SE	NBP1-97712MFV500
mFluor Violet 610 SE	NBP1-97712MFV610

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NB110-68133

Napsin A Antibody (TMU-Ad02) - BSA Free

(1 Review)

(3 Publications)

0.1 ml

NB110-68133

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-68133AF350
Alexa Fluor 405	NB110-68133AF405
Alexa Fluor 488	NB110-68133AF488
Alexa Fluor 532	NB110-68133AF532
Alexa Fluor 594	NB110-68133AF594
Alexa Fluor 647	NB110-68133AF647
Alexa Fluor 700	NB110-68133AF700
Alexa Fluor 750	NB110-68133AF750
Allophycocyanin	NB110-68133APC
Biotin	NB110-68133B
CoraFluor 1	NB110-68133CL1
DyLight 350	NB110-68133UV
DyLight 405	NB110-68133V
DyLight 488	NB110-68133G
DyLight 550	NB110-68133R
DyLight 594	NB110-68133DL594
DyLight 650	NB110-68133C
DyLight 680	NB110-68133FR
DyLight 755	NB110-68133IR
FITC	NB110-68133F
HRP	NB110-68133H
Janelia Fluor 525	NB110-68133JF525
Janelia Fluor 549	NB110-68133JF549
Janelia Fluor 646	NB110-68133JF646
Janelia Fluor 669	NB110-68133JF669
PE	NB110-68133PE
PerCP	NB110-68133PCP
mFluor Violet 450 SE	NB110-68133MFV450
mFluor Violet 500 SE	NB110-68133MFV500
mFluor Violet 610 SE	NB110-68133MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-68133SS
Azide and BSA Free	NBP2-80866

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NB110-74571

Striatin Antibody - BSA Free

(7 Publications)

0.1 ml

NB110-74571

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-74571SS

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NBP1-41186

BRCA1 Antibody (8F7) - Azide and BSA Free

(10 Publications)

100 ul

NBP1-41186

Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382 was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB110-82375

Mucolipin 1 Antibody - BSA Free

(1 Review)

(4 Publications)

0.1 ml

NB110-82375

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-82375AF350
Alexa Fluor 405	NB110-82375AF405
Alexa Fluor 488	NB110-82375AF488
Alexa Fluor 532	NB110-82375AF532
Alexa Fluor 594	NB110-82375AF594
Alexa Fluor 647	NB110-82375AF647
Alexa Fluor 700	NB110-82375AF700
Alexa Fluor 750	NB110-82375AF750
Allophycocyanin	NB110-82375APC
Biotin	NB110-82375B
CoraFluor 1	NB110-82375CL1
DyLight 350	NB110-82375UV
DyLight 405	NB110-82375V
DyLight 488	NB110-82375G
DyLight 550	NB110-82375R
DyLight 594	NB110-82375DL594
DyLight 650	NB110-82375C
DyLight 680	NB110-82375FR
DyLight 755	NB110-82375IR
FITC	NB110-82375F
HRP	NB110-82375H
Janelia Fluor 525	NB110-82375JF525
Janelia Fluor 549	NB110-82375JF549
Janelia Fluor 646	NB110-82375JF646
Janelia Fluor 669	NB110-82375JF669
PE	NB110-82375PE
PerCP	NB110-82375PCP
mFluor Violet 450 SE	NB110-82375MFV450
mFluor Violet 500 SE	NB110-82375MFV500
mFluor Violet 610 SE	NB110-82375MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-82375SS

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NBP2-22116

Cytokeratin 19 Antibody (4E8) - BSA Free

(2 Publications)

0.1 ml

NBP2-22116

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.

Western Blot: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Western blot analysis using Cytokeratin 19 mAb against human Cytokeratin 19 (AA: 115-269) recombinant protein. (Expected MW is 43.1 kDa)

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 19 mouse mAb with DAB staining.

ELISA: Cytokeratin 19 Antibody (4E8) [NBP2-22116] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22116AF350
Alexa Fluor 405	NBP2-22116AF405
Alexa Fluor 488	NBP2-22116AF488
Alexa Fluor 532	NBP2-22116AF532
Alexa Fluor 594	NBP2-22116AF594
Alexa Fluor 647	NBP2-22116AF647
Alexa Fluor 700	NBP2-22116AF700
Alexa Fluor 750	NBP2-22116AF750
Allophycocyanin	NBP2-22116APC
Biotin	NBP2-22116B
CoraFluor 1	NBP2-22116CL1
DyLight 350	NBP2-22116UV
DyLight 405	NBP2-22116V
DyLight 488	NBP2-22116G
DyLight 550	NBP2-22116R
DyLight 594	NBP2-22116DL594
DyLight 650	NBP2-22116C
DyLight 680	NBP2-22116FR
DyLight 755	NBP2-22116IR
FITC	NBP2-22116F
HRP	NBP2-22116H
Janelia Fluor 525	NBP2-22116JF525
Janelia Fluor 549	NBP2-22116JF549
Janelia Fluor 646	NBP2-22116JF646
Janelia Fluor 669	NBP2-22116JF669
PE	NBP2-22116PE
PerCP	NBP2-22116PCP
mFluor Violet 450 SE	NBP2-22116MFV450
mFluor Violet 500 SE	NBP2-22116MFV500
mFluor Violet 610 SE	NBP2-22116MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22116SS
Azide and BSA Free	NBP2-80689

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NBP2-24716

MTSS1 Antibody - BSA Free

(2 Publications)

0.1 mg

NBP2-24716

No Mtss1 was found in endothelial cells at E14.5, and no vascular defects were observed in Mtss1-conditional knockout (KO) mice.(A) Fluorescence in situ hybridization for Plxnd1 mRNA (green) and Mtss1 mRNA (red) in the brain and dorsal trunk at E14.5. White dotted boxes are shown in the inset image on the bottom. Scale bars, 100 μm for brain, 50 μm for dorsal trunk. (B) 3D vascular reconstruction analysis images after CD31 immunostaining and tissue clearing obtained using multifunctional fast confocal microscopy Dragonfly 502w. (C) Western blotting to analyze Mtss1 expression after AP-Sema3E (2 nM) treatment in human umbilical vein endothelial cells (HUVECs) or human cortical microvessel endothelial cells (HCMEC/D3). Error bars, mean +/- SEM; ns p>0.05 by Mann‒Whitney test; AP, n = 4, AP-Sema3E, n = 4 for HUVECs, ns p>0.05 by Student’s t-test; AP, n = 4, AP-Sema3E, n = 4 for HCMEC/D3s in four dependent experiments.Figure 8—figure supplement 1—source data 1.Western blots shown in Figure 8—figure supplement 1C.Western blots shown in Figure 8—figure supplement 1C. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of Mtss1 induces I-BAR domain-dependent morphological changes in COS7 cells, generating protrusions.(A) Western blot images showing that weakly expression of endogenous Mtss1 was not altered by overexpression of Plexin-D1 with or without Sema3E in COS7 cells. Asterisk indicates nonspecific band. (B) Quantification of the band intensity in (A). Error bars, mean +/- SEM; ns p>0.05 by two-way ANOVA with Bonferroni’s post hoc correction for multiple comparisons; n = 3. (C) Schematics describing the full-length construct of Mtss1-myc and its deletion mutant constructs (Mtss1 delta I-BAR-myc, Mtss1 delta WH2-myc, and I-BAR-myc). (D) Immunocytochemistry images taken after overexpression of each construct. Constructs show the I-BAR domain leading to diverse cell protrusion morphologies. Some of the protrusions were excessively spiked or thin and long (arrowheads). Overexpression of the I-BAR domain only (I-BAR-myc) can induce extreme protrusion structures. Scale bar, 20 μm.Figure 3—figure supplement 1—source data 1.Western blots shown in Figure 3—figure supplement 1A.Western blots shown in Figure 3—figure supplement 1A. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The absence of Mtss1 does not affect medium spiny neuron (MSN) survival, dendritic arborization, and Plexin-D1 expression during striatonigral pathway development.(A) Immunohistochemistry staining for cleaved caspase 3 (CC3) in the striatum of wild-type (WT) or Mtss1 conditional knockout (cKO) mice. The white dotted boxes on the left images are shown in the inset images on the right at a better resolution. Scale bar, 25 μm. (B) Quantification of cell death by the number of CC3-positive cells in a 1 mm2 area covering the dorsal part of the striatum in WT or Mtss1 cKO mice. Error bars, mean +/- SEM; ns p>0.05 by Mann‒Whitney test; WT, n = 20; Mtss1 cKO mice, n = 20 (five sections/mouse). (C) Representative images of Golgi staining at low (top panels) and high (bottom panels) magnification. Scale bars, 100 μm. Sholl analysis of dendritic morphology (D) and dendritic length (E) performed by using Neurolucida360 in 3D analysis. Error bars, mean +/- SEM; ns p>0.05 by Student’s t-test; WT, n = 12, and Mtss1 cKO mice, n = 15, from three mice. (F, G) Western blot images and quantification of Plexin-D1 expression in the striatum of WT or Mtss1 cKO mice at P5. Error bars, mean +/- SEM; *p0.05 by Mann‒Whitney test; WT, n = 20; Mtss1 cKO mice, n = 20 (five sections/mouse). (C) Representative images of Golgi staining at low (top panels) and high (bottom panels) magnification. Scale bars, 100 μm. Sholl analysis of dendritic morphology (D) and dendritic length (E) performed by using Neurolucida360 in 3D analysis. Error bars, mean +/- SEM; ns p>0.05 by Student’s t-test; WT, n = 12, and Mtss1 cKO mice, n = 15, from three mice. (F, G) Western blot images and quantification of Plexin-D1 expression in the striatum of WT or Mtss1 cKO mice at P5. Error bars, mean +/- SEM; *p<0.05 by Student’s t-test; WT mice, n = 3, and Mtss1 cKO mice, n = 4. (H) Plexin-D1 expression in MSNs obtained from the striatum of WT or Mtss1 cKO mice at P0 and measured at DIV6 in culture. (I) Quantification of the western blots shown in (H). Error bars, mean +/- SEM; *p<0.05, by Student’s t-test; n = 3 for WT, n = 3 for KO in three independent experiments.Figure 7—figure supplement 3—source data 1.Raw uncropped western blot & gel images.Western blots shown in Figure 7—figure supplement 3F and H.Raw uncropped western blot & gel images.Western blots shown in Figure 7—figure supplement 3F and H. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Pm, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6108A

Alt Catalog# IMG-6108A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24716SS

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NB100-1687

MRCL3 [p Ser19, p Ser20] Antibody - BSA Free

(1 Review)

0.05 mg

NB100-1687

Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Affinity purified phosphospecific antibody to monophosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19 was used at a 1:1000 dilution to detect myosin light chain by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated or insulin or CLA treated according as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gta- Rabbit IgG (611-103-122) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.

Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 l of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.

Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody: Myosin pS19/pS20 primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Regulatory Light Chain Phospho. Other band(s): None.

Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.

affinity purified anti-Monophosphorylated RLC Smooth and Non-Muscle Myosin pS19/20 antibody was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Immunohistochemistry with anti-myosin pS19/pS20 antibody showing strong cytoplasmic staining of myocytes in mouse heart muscle 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-myosin pS19/pS20 antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.

Affinity Purified Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13uL or 20 ug of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG () preceded color development using Amersham's substrate system. Other detection methods will yield similar results. Data courtesy of the Alliance for Cellular Signaling (//www.signaling-gateway.org).

Western blot of Rabbit Anti-Myosin pS19/pS20 primary antibody. Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody: Myosin pS19/pS20 primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking: for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Regulatory Light Chain Phospho. Other band(s): None.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

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Modification

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Clone

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Species Glossary

MCT8/SLC16A2 Antibody - BSA Free

Vimentin Antibody - BSA Free

S100A10 Antibody - BSA Free

Profilin 1 Antibody (OTI1D5)

Cytokeratin 19 Antibody (RCK108) - BSA Free

Napsin A Antibody (TMU-Ad02) - BSA Free

Striatin Antibody - BSA Free

BRCA1 Antibody (8F7) - Azide and BSA Free

Mucolipin 1 Antibody - BSA Free

Cytokeratin 19 Antibody (4E8) - BSA Free

MTSS1 Antibody - BSA Free

MRCL3 [p Ser19, p Ser20] Antibody - BSA Free