| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-213SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33148 | |||
| IHC-Prediluted | NBP2-44386 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56709SS | |||
| Azide and BSA Free | NBP2-80613 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89300-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78381SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68281SS | |||
| Azide and BSA Free | NBP2-80993 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92156-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54791SS | |||
| Azide and BSA Free | NBP2-80822 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64792SS | |||
![Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.](http://images.novusbio.com/fullsize/Smac-Antibody-78-1-118-Western-Blot-NB500-213-img0002.jpg "Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0011.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human skin stained with MART-1 Monoclonal Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0007.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0005.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0006.jpg)
![Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Frozen-NBP2-15197-img0010.jpg)
![Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK and the studied compounds, lysed and subjected to Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-01230-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK and the studied compounds, lysed and subjected to Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-01230-4) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) [NB100-56709] - Tissue section of normal human breast using 5 ug/mL concentration of . Very strong diffused as well as granular immunopositivity of Caspase 3 was observed specifically in the cytoplasmic of ductal /acinar epithelial cells.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunohistochemistry-Paraffin-NB100-56709-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody (31A893) [NB100-56709] - Left panel: Untreated Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with at 10 ug/mL overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunocytochemistry-Immunofluorescence-NB100-56709-img0008.jpg)
![Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Analysis for human Caspase-3 using HL60 lysates with Caspase-3 Antibody (31A893) at 2 ug/mL dilution. NB100-56709 only detects a 32 kD Caspase-3 corresponding to pro-Caspase-3.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0005.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Analysis of Caspase-3 in Jurkat cells. Cells were treated with 2 uM staurosporine for different time periods. Caspase-3 activation is detected in Western blots by the presence of Caspase-3 cleavage fragments. These antibodies detect both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0006.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) [NB100-56709] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 5 ug/mL of mouse monoclonal Caspase-3 Antibody (31A893) (NB100-56709, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunohistochemistry-Paraffin-NB100-56709-img0007.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Lemongrass extract is dependent on the production of oxidative stress to induce apoptosis. (E) E6-1 cells treated for 3 hrs with LG with or without NAC, lysed, & subjected to SDS-PAGE. Cells then transferred to a PVDF membrane & probed for the specific proteins. Bands visualized with a chemiluminescence reagent. Statistical calculations performed using Two-Way ANOVA multiple comparison for (A) & One-Way ANOVA multiple comparison for (B–C). **p < 0.01 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-31020241535657.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Functioning FADD protein is required to induce apoptosis in cancer cells treated with lemongrass extract(A) U-937 & (B) DN FADD Jurkat cells were treated for 12 hours & 48 hours, respectively, with the specified treatments, lysed, & subjected to SDS-PAGE. Cells were then transferred to a PVDF membrane & probed for the specific proteins. Bands were visualized with a chemiluminescence reagent. (C) DN FADD Jurkat cells were treated for 48 hours with the specified doses & stained with Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (D) DN FADD Jurkat cells were treated with H2DCFDA following treatments with paraquat (PQ), LG, or WT for 3 hours. Results were obtained using the image-based cytometry with the Y-axis representative of percent of cells positive for DCF. Values are expressed as a mean ± SD from three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison for (C) & One-Way ANOVA multiple comparison for (D). ***p < 0.001 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-310202415525230.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Functioning FADD protein is required to induce apoptosis in cancer cells treated with lemongrass extract(A) U-937 & (B) DN FADD Jurkat cells were treated for 12 hours & 48 hours, respectively, with the specified treatments, lysed, & subjected to SDS-PAGE. Cells were then transferred to a PVDF membrane & probed for the specific proteins. Bands were visualized with a chemiluminescence reagent. (C) DN FADD Jurkat cells were treated for 48 hours with the specified doses & stained with Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (D) DN FADD Jurkat cells were treated with H2DCFDA following treatments with paraquat (PQ), LG, or WT for 3 hours. Results were obtained using the image-based cytometry with the Y-axis representative of percent of cells positive for DCF. Values are expressed as a mean ± SD from three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison for (C) & One-Way ANOVA multiple comparison for (D). ***p < 0.001 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-310202415535124.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Analysis in human skeletal muscle and liver tissues. Corresponding FLNC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Analysis in human skeletal muscle and liver tissues. Corresponding FLNC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FLNC Antibody [NBP1-89300] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining shown in green.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89300-img0009.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0015.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0006.jpg)
![Immunohistochemistry-Frozen: FLNC Antibody [NBP1-89300] - Mouse muscle vasus transverse cryosection stained with Filamin C antibody (NBP1-89300). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Frozen-NBP1-89300-img0012.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staning of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0013.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0014.jpg)
![Immunohistochemistry: FAT4 Antibody - BSA Free [NBP1-78381] - FAT4 Antibody [NBP1-78381] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FAT4-Antibody-Knockout-Validated-NBP1-78381-img0005.jpg "<b>Genetic Strategies Validation. </b>Immunohistochemistry: FAT4 Antibody - BSA Free [NBP1-78381] - FAT4 Antibody [NBP1-78381] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: FAT4 Antibody [NBP1-78381] - IHC staining of metastatic lung nodules in the nude mice model. Representative images show IHC staining with anti-FAT4 antibody of lung sections from BGC-823-FAT4-KO and BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 i1/4m. Image collected and cropped by Citeab from the following publication (Low FAT4 expression is associated with a poor prognosis in gastric cancer patients. Oncotarget (2018) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FAT4-Antibody-Knockout-Validated-NBP1-78381-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAT4 Antibody [NBP1-78381] - Antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/FAT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78381-img0003.jpg)
![Immunohistochemistry: FAT4 Antibody [NBP1-78381] - IHC analysis of FAT4 in mouse prostate cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/FAT4-Antibody-Immunohistochemistry-NBP1-78381-img0004.jpg)
![Western Blot: FAT4 Antibody - BSA Free [NBP1-78381] - Expression of selected genes in BCC subsets. (A) Real time PCR for GPR64, FAT4, TMEM98, ALOX5 AP, DUSP4, RAP1A & FN1. The RNA was isolated from Oct4hi, Octmed & Oct4low subsets from MDA-MB-231 stably transfected with pEGFP1-Oct3/4. The results are shown as relative to beta -actin expression with mean ± SD for four independent experiments. (B) Western blots for GPR64, TMEM98, FAT4, RAP1A & FN1 using whole cell extracts expression from Oct4hi, Octmed & Oct4low, isolated as for ‘A’. The results represent four different independent experiments. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-18834-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78381_rabbit-polyclonal-fat4-antibody-310202415392572.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0012.jpg "Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.")
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0011.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0003.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0005.jpg)
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0009.jpg)
![Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Simple-Western-NB110-68281-img0010.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) antagomiR in rat vascular smooth muscle cells (A7r5) induced angiogenesis & proliferation but suppressed senescence. a Schematic of the protocol for transfection of A7r5 VSMCs with a miRNA antagomiR control (antagomiR NC) or a miR-214 antagomiR & processed at the indicated times. b The effects of miR-214 antagomiR on VSMC cell proliferation were tested by CCK-8 assay. c Angiogenetic mRNA expression levels of NOS3, VEGFA, CXCL12 & CXCR4 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). d Senescence-associated mRNA expression of TERT, TERF1 & TERF2 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). e, G Representative western blots depicting TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking expression in antagomiR NC or miR-214 antagomiRs transfected cells. f, h Normalized expression of TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking (n = 3). i Senescence-associated beta -galactosidase staining demonstrating senescence in SCR or miR-214 transfected cells. j Bar graphs show quantification of relative of SA-beta -gal positive cells (n = 3). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; ns, non-significance (Two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-68281_mouse-monoclonal-trf-1-antibody-57-6-310202416212346.jpg)
![Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Analysis in human cerebral cortex and liver tissues. Corresponding Myosin 5a RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Immunohistochemistry-Paraffin-NBP1-92156-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Analysis in human cerebral cortex and liver tissues. Corresponding Myosin 5a RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Myosin 5a Antibody [NBP1-92156] - Staining of human cell line A-431 shows localization to microtubule organizing center & focal adhesion sites. Antibody staining shown in green.](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92156-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human kIdney shows moderate memberanous positivity in cells in tubules.](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Immunohistochemistry-Paraffin-NBP1-92156-img0013.jpg)
![Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Immunohistochemistry-Paraffin-NBP1-92156-img0002.jpg)
![Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebral cortex shows strong membranous positivity in neuropil.](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Immunohistochemistry-Paraffin-NBP1-92156-img0006.jpg)
![Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human hippocampus shows strong cytoplasmic positivity in pyramidal cells.](http://images.novusbio.com/fullsize/Myosin-5a-Antibody-Immunohistochemistry-Paraffin-NBP1-92156-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Ki67/MKI67 Antibody (1297A) NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0007.jpg "Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Ki67/MKI67 Antibody (1297A) NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in Ki67/MKI67 knockout (KO) HeLa cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # NBP2-54791) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Knockout-Validated-NBP2-54791-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Ki67/MKI67 Antibody [1297A] conjugated to Alexa Fluor 647 (NBP2-54791AF647) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0010.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki-67/MKI67 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Ki-67/MKI67 Monoclonal Antibody at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunohistochemistry-Paraffin-NBP2-54791-img0002.jpg)
![Flow (Intracellular): Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki-67/MKI67 Antibody (1297A) [NBP2-54791] - An intracellular stain was performed on Jurkat Cells with Ki-67/MKI67 (1297A) antibody NBP2-54791 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Flow-Intracellular-NBP2-54791-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Ki67/MKI67 (1297A) at 2.0 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0003.jpg)
![Flow Cytometry: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - An intracellular stain was performed on HeLa cells with Ki67/MKI67 Antibody [1297A] NBP2-54791APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Flow-Cytometry-NBP2-54791-img0005.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67/MKI67 immunoreactivity in mouse small intestine. NBP2-54791 was diluted to 0.5ug/mL in Antibody Diluent and was left on sections for 1h at room temperature. Secondary was Horse Anti-Rabbit HRP. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-54791_rabbit-monoclonal-ki67-mki67-antibody-1297a-1552023121119.jpg)
![Immunohistochemistry-Paraffin: Rabbit Monoclonal Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67 immunoreactivity in FFPE sections of human tonsil. NBP2-54791 was used at 500ng per mL and was left on tissue sections for 1hr at room temperature. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-54791_rabbit-monoclonal-ki67-mki67-antibody-1297a-immunohistochemistry-paraffin-15202512223..jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.](http://images.novusbio.com/fullsize/F-Actin-Antibody-NH3-Immunocytochemistry-Immunofluorescence-NB100-64792-img0001.jpg "Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792] - Human inner ear tissue section stained with Actin Gamma 1 antibody at 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse at 1:200. Nuclei stained with DAPI. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-NH3-Immunohistochemistry-Paraffin-NB100-64792-img0002.jpg)
![Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Immunohistochemistry-Paraffin-NB500-330-img0009.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0007.jpg)
![Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Immunohistochemistry-Frozen-NB500-330-img0008.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0002.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0004.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0003.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) FITC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0005.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0015.jpg "Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.")
![Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 (3) for further details).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0013.jpg)
![Functional (Inhibition): RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29321) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-p-Ser529-p-Ser536-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29321-img0012.jpg)