| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-316SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56583SS | |||
| Azide and BSA Free | NBP2-80872 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-644SS | |||
| Azide and BSA Free | NBP2-80970 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1317SS | |||
| Azide and BSA Free | NBP2-80649 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80659-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81556-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-586-0.025ml | |||
![Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - p53 repression of autophagy is PINK1-dependent. LC3-2/LC3-1 (a,b, N = 12), p62/SQSTM1 (a,c, N = 12), TIM23 (a,d, N = 12), TOM20 (a,e, N = 12) and HSP60 (a,f, N = 12) protein levels were analyzed in control (PINK+/+) or Pink1-deficient (PINK-/-) HAP cells treated with vehicle (Ct), pifithrin-alpha (PFT, 30 uM, 4 h) or CCCP (CP, 10 uM, 6 h) as described in the Methods. Bars represent the means +/- SEM of 4 independent experiments performed in triplicates and are expressed as percent of control HAP1 (HAP+/+, Ct) cells. Actin expression is provided as a representative gel loading control. Statistical analyses were performed with GraphPad Prism software by using unpaired Student's t-test. Significant differences are: ns not significant, *p < 0.05, ** p < 0.01 and ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-017-0016-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49956-img0006.jpg "Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - p53 repression of autophagy is PINK1-dependent. LC3-2/LC3-1 (a,b, N = 12), p62/SQSTM1 (a,c, N = 12), TIM23 (a,d, N = 12), TOM20 (a,e, N = 12) and HSP60 (a,f, N = 12) protein levels were analyzed in control (PINK+/+) or Pink1-deficient (PINK-/-) HAP cells treated with vehicle (Ct), pifithrin-alpha (PFT, 30 uM, 4 h) or CCCP (CP, 10 uM, 6 h) as described in the Methods. Bars represent the means +/- SEM of 4 independent experiments performed in triplicates and are expressed as percent of control HAP1 (HAP+/+, Ct) cells. Actin expression is provided as a representative gel loading control. Statistical analyses were performed with GraphPad Prism software by using unpaired Student's t-test. Significant differences are: ns not significant, *p < 0.05, ** p < 0.01 and ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-017-0016-0), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49956] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-Paraffin-NBP1-49956-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - Detection of Human and Mouse Sequestosome-1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49956-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - Evaluation of mitophagy control by p53 in mice brain. p62/SQSTM1 (N = 9) protein levels were analyzed in control (p53+/+) or TP53-deficient (p53-/-) knockout mice brains as described in the Methods. Bars represent the means +/- SEM of 3 independent experiments performed in triplicate and are expressed as percent of control (p53+/+) cells. Actin expression is provided as a representative gel loading control. Statistical analyses were performed with GraphPad Prism software by using unpaired Student's t-test. Significant differences are: ** p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-017-0016-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49956-img0005.jpg)
![Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Western-Blot-NB100-239-img0006.jpg "Western Blot: AMPK alpha 1 Antibody [NB100-239] - Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human smooth muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 (NB100-239). Detection: DAB](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunohistochemistry-NB100-239-img0005.jpg)
![Immunoprecipitation: AMPK alpha 1 Antibody [NB100-239] - Detection of human AMPK alpha 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AMPK alpha 1 antibody NB100-239 (lot 2) used for IP at 3 ug per reaction. AMPK alpha 1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated AMPK alpha 1, NB100-239 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-Immunoprecipitation-NB100-239-img0007.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0002.jpg "Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.")
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0001.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys & urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies & merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) & distal (THP) renal tubular epithelial cells & renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder & left & right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) & uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) & merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-749_mouse-monoclonal-aquaporin-1-aqp1-antibody-1-a5f6-31020241617140.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - USSC administration recovered aquaporin1 (AQP1) expression in the choroid plexus after PHH. (A) Representative immunofluorescence image of cryosections labeled with AQP1 specific antibody (arrows showing choroid epithelium immunoreactivity) on postnatal days 7. Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & images from left to right respectively at postnatal day 7. CPE (Choroid plexus epithelial cells). Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (B) Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & the images from left to right respectively at day 14. Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (C) Representative immunofluorescence image of cryosections labeled with aquaporin1. As seen in the high power image (panel-B above), the strong AQP1 immunoreactivity is evident over the entire choroid plexus in the control, reduced signal in IVH followed by recovered strong AQP1 expression observed in images from left to right respectively at day 14. Images were taken at 4× objective; 20 μm sections. The scale bar is 500 μm. Fluorescence images were taken using “Nikon Eclipse 90i microscope” (Nikon Instruments, Japan). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33897371), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-749_mouse-monoclonal-aquaporin-1-aqp1-antibody-1-a5f6-310202416171417.jpg)
![Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Simple-Western-NB100-316-img0005.jpg "Simple Western: FANCD2 Antibody (FI-17) [NB100-316] - Image shows a specific band for FANCD2 in 1 mg/mL of HeLa cell lysate using FANCD2 Antibody (FI-17). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - MCF7 cell line (lane 1, molecular weight: 164.1 KDa) and HeLa whole cell extract (lane 2) using FANCD2 Antibody (FI-17).](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0003.jpg)
![Western Blot: FANCD2 Antibody (FI-17) [NB100-316] - Expression of FANCD2 (Molecular weight: 164.1 KDa) in different cell lines. Proliferating cells were treated with 10-Gy IR. 50 ug protein was loaded each lane. FANCD2 Antibody (FI-17) at 1:1000.](http://images.novusbio.com/fullsize/FANCD2-Antibody-FI-17-Western-Blot-NB100-316-img0004.jpg)
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Western-Blot-NB100-56583-img0004.jpg "Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.")
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0008.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0003.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0005.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0006.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of NFkB p105/p50 (2J10D7) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nb100-56583_mouse-monoclonal-nfkb-p105-p50-antibody-2j10d7-imgenex-img-5812a-2210202585646.jpg)
![Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunohistochemistry-NB500-644-img0005.jpg "Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (8E2) [NB500-644] - Immunocytochemical analysis using Survivin Antibody (8E2) [NB500-644] was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunocytochemistry-Immunofluorescence-NB500-644-img0004.jpg)
![Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Flow-Intracellular-NB500-644-img0006.jpg)
![Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.](http://images.novusbio.com/fullsize/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0003.jpg "Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.")
![Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) [NB600-1317] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaining. This antibody clone specifically stained the membranes of the cells close to the bases of the crypts/villi which are known to actively migrate towards the tip of villi.](http://images.novusbio.com/fullsize/CD44-Antibody-OX-50-Immunohistochemistry-Paraffin-NB600-1317-img0005.jpg)
![Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0002.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.")
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/fullsize/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/fullsize/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - SOX9 monoclonal antibody (M02), clone 3C10 Analysis of SOX9 expression in HepG2.](http://images.novusbio.com/fullsize/SOX9-Antibody-3C10-Western-Blot-H00006662-M02-img0013.jpg "Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - SOX9 monoclonal antibody (M02), clone 3C10 Analysis of SOX9 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SOX9-Antibody-3C10-Immunocytochemistry-Immunofluorescence-H00006662-M02-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.7 ug/ml.](http://images.novusbio.com/fullsize/SOX9-Antibody-3C10-Immunohistochemistry-Paraffin-H00006662-M02-img0010.jpg)
![Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody (M02), clone 3C10.Lane 1: SOX9 transfected lysate(56.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SOX9-Antibody-3C10-Western-Blot-H00006662-M02-img0014.jpg)
![Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02] - analysis of SOX9 in formalin-fixed, paraffin-embedded human fetal developing pancreas tissue using anti-SOX9 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/SOX9-Antibody-3C10-Immunohistochemistry-Paraffin-H00006662-M02-img0006.jpg)
![Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SOX9 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SOX9-Antibody-3C10-Immunoprecipitation-H00006662-M02-img0011.jpg)
![ELISA: SOX9 Antibody (3C10) [H00006662-M02] - Detection limit for recombinant GST tagged SOX9 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SOX9-Antibody-3C10-ELISA-H00006662-M02-img0015.jpg)
![Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP1-80659-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP1-80659] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80659-img0009.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0015.jpg)
![Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP1-80659-img0008.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human prostate shows moderate cytoplasmic positivity in glandular and in smooth muscle cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0012.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0013.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP1-80659-img0014.jpg)
![Western Blot: TOMM20 Antibody [NBP1-81556] - Analysis in human cell lines HEK293 and MCF-7. Corresponding TOMM20 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP1-81556-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: TOMM20 Antibody [NBP1-81556] - Analysis in human cell lines HEK293 and MCF-7. Corresponding TOMM20 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: TOMM20 Antibody [NBP1-81556] - Analysis in mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Western-Blot-NBP1-81556-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody [NBP1-81556] - Z-stack confocal images with SRRF (super-resolution radial fluctuations) analysis of Tomm20, an outer mitochondrial membrane protein, in HeLa cells. Tomm20 was detected in HeLa cells using 1 ug/ml Rb anti-Tomm20 (NBP1-81556) and 1:500 JF646-conjugated anti-Rb secondary antibody (red). Cells were counterstained with Dapi (blue). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81556-img0021.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human duodenum shows moderate to strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody [NBP1-81556] - RPE1 cells were fixed with 4% PFA, permeablized and blocked in 2.5% BSA with 0.2% Triton-X. Primary antibody was diluted 1:200 in PBS containing 2.5% BSA overnight at 4 deg C. Cells were incubated with anti-rabbit secondary antibody and counterstained with hoescht 33342. Image was collected using Zeiss 780 LSM. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81556-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TOMM20 Antibody [NBP1-81556] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81556-img0008.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human endometrium shows moderate to strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0013.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human prostate shows moderate to strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0014.jpg)
![Immunohistochemistry-Paraffin: TOMM20 Antibody [NBP1-81556] - Staining of human cerebral cortex shows moderate to strong positivity in mitochondria in neurons.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Immunohistochemistry-Paraffin-NBP1-81556-img0015.jpg)
![Simple Western: TOMM20 Antibody [NBP1-81556] - Simple Western lane view shows a specific band for TOMM20 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TOMM20-Antibody-Simple-Western-NBP1-81556-img0006.jpg)
![Simple Western: TOMM20 Antibody [NBP1-81556] - Electropherogram image(s) of corresponding Simple Western lane view. TOMM20 antibody was used at 1:30 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/TOMM20-Antibody-Simple-Western-NBP1-81556-img0007.jpg)
![Western Blot: TOMM20 Antibody [NBP1-81556] - Quantification of LC3B-II & TOM20 proteins in WT & Ts1Cje hippocampus. Hippocampal proteins from WT & Ts1Cje mice pairs were analyzed in Western blot with anti-LC3B or anti-TOM20 antibody. A LC3B western blot showing WT & Ts1Cje littermate pairs analyzed & total protein loaded. The ratio LC3B-II/LC3B-I is shown as the mean ± SEM (WT: 0.8714 ± 0.04154; Ts1Cje: 0.6500 ± 0.06802; p = 0.0152, Student’s t-test, n = 7 for WT & n = 6 for Ts1Cje). B TOM20 western blot showing WT & Ts1Cje littermate pairs analyzed & total protein loaded. The signals were normalized to the corresponding total protein loaded & the mean ± SEM values are shown (WT: 1.024 ± 0.08799; Ts1Cje: 1.412 ± 0.1452; p = 0.0414, Student’s t-test, n = 7 animals per genotype) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34034796), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81556_rabbit-polyclonal-tomm20-antibody-310202415171331.jpg)
![Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Dual-RNAscope-ISH-IHC-NB600-586-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-NB600-586-img0001.jpg)
![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Paraffin-NB600-586-img0005.jpg)
![Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586] - Canine brain/spinal cord. VWF antibody in green. A pericyte marker in red. Alexa Fluor conjugated secondary antibodies were used for visualization. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Frozen-NB600-586-img0008.jpg)
![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Paraffin-NB600-586-img0006.jpg)