Product Search for c Kit: Novus Biologicals

255-SC

Recombinant Human SCF Protein

(209 Publications)

10 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	255-SC-001MG/CF
Carrier Free	255-SC-010/CF
Carrier Free	255-SC-050/CF
Carrier Free	255-SC-200/CF

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455-MC

Recombinant Mouse SCF Protein

(189 Publications)

10 μg

455-MC

1 μg/lane of Recombinant Mouse SCF/c-kit was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	455-MC-010/CF
Carrier Free	455-MC-050/CF
Carrier Free	455-MC-500/CF
Carrier Free	455-MC-020/CF
Carrier Free	455-MC-100/CF

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AF1356

CD117/c-kit Antibody [Unconjugated]

(4 Reviews)

(128 Publications)

100 μg

AF1356

CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9-2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Localization of MAFB in mouse testes.(A) Localization of MAFB in E18.5 mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), GATA4, or STAR is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. G; germ cells. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells and Sertoli cells. (B) Localization of MAFB in adult mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), KIT, SCP3, PNA Lectin, or vimentin is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.

Spermatogenesis development of adult Mafb-cKO mice.Six-week-old mice were injected with tamoxifen and testes of cKO mice at 3- and 8-months of age were examined compared to those of age-matched controls (n = 4 for each group). (A) Testicular sections stained with Periodic acid-Schiff (PAS). (B) Immunostaining with various testicular cell types; undifferentiated spermatogonia (GFRA1 and PLZF), differentiated spermatogonia (KIT+ inside the tubule), spermatocytes (SCP3), spermatids (PNA-Lectin), Sertoli cells (SOX9 and Vimentin), and Leydig cells (KIT+ outside the tubule and STAR). The color of markers is indicated in the left boxes in panels. Colored arrows corresponding to the boxed markers are shown. (C) The expression level of the genes involved in testis function and germ cell development. The marker genes representing undifferentiated spermatogonia (Nanos3), differentiated spermatogonia (c-Kit), undifferentiated and differentiated spermatogonia (Sohlh1), differentiated spermatogonia and preleptotene spermatocytes (Stra8), spermatids (Prm2), Leydig cells (Hsd3b1), and Sertoli cells (Sox9) were analyzed by qRT-PCR. Each reaction was performed in duplicate for each gene. The data represent the means±SEM and are shown as relative mRNA expression after normalization to Hprt. *PAF1356) at 5 μg/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in esophagus in mouse embryo.

c-kit-, F4/80- or Ly-6G-positive cell numbers in cornea after alkali injury. Corneal tissues were obtained 4 days after injury from vehicle- or SDF-1 alpha -treated BABL/c mice, and the tissues from 7 to 8 mice were combined and were subjected to analysis using a flow cytometer after being immunostained with anti-c-kit, anti-F4/80, or anti-Ly-6G antibody. Isotype IgG derived from the same species of the test antibody was used as negative control. Representative results from three to four tests of intracorneal infitlration of c-kit- (A), F4/80- (B), or Ly-6G-positive cells (C) from either vehicle- (left plot) or SDF-1 alpha -treated mice (right plot) are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21850188), licensed under a CC-BY license. Not internally tested by R&D Systems.

CD117/c-kit was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 0.5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1356U-100UG
Alexa Fluor 405	AF1356V-100UG
Alexa Fluor 488	AF1356G-100UG
Alexa Fluor 532	AF1356X-100UG
Alexa Fluor 594	AF1356T-100UG
Alexa Fluor 647	AF1356R-100UG
Alexa Fluor 700	AF1356N-100UG
Alexa Fluor 750	AF1356S-100UG
Allophycocyanin	AF1356A
Biotin	BAF1356
CoraFluor 1	AF1356CL1
DyLight 350	AF1356D
DyLight 405	AF1356E
DyLight 488	AF1356K
DyLight 550	AF1356L
DyLight 594	AF1356M
DyLight 650	AF1356W
DyLight 680	AF1356Y
DyLight 755	AF1356Z
FITC	AF1356F
HRP	AF1356H
Janelia Fluor 525	AF1356JF525
Janelia Fluor 549	AF1356I
Janelia Fluor 646	AF1356J
Janelia Fluor 669	AF1356JF669
PE	AF1356P
PerCP	AF1356C
mFluor Violet 450 SE	AF1356MFV450
mFluor Violet 500 SE	AF1356MFV500
mFluor Violet 610 SE	AF1356MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1356-SP

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DTA00D

Human TNF-alpha Quantikine ELISA Kit

(561 Publications)

1 Kit

DTA00D

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	STA00D
HRP	PDTA00D

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	STA00D
Carrier Free	PDTA00D

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DY208

Human IL-8/CXCL8 DuoSet ELISA, 15 Plate

(507 Publications)

1 Kit (for 5 Plates)

DY208

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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D8000C

Human IL-8/CXCL8 Quantikine ELISA Kit

(453 Publications)

1 Kit

D8000C

RT-qPCR for a set of cytokines (CSF2, IL6, IL8 and IL1B) and chemokines (CXCL1 and CXCL6) of total RNA samples derived from mono- and co-cultures in a transwell assay.The respective co- (+) or mono- (-) culture is indicated on the X-axis. The respective analyzed cytokine or chemokine is indicated in the header of each graph. Expression values are shown in arbitrary units (AU) and have been normalized to beta-2 microglobulin (B2M) mRNA copies. Experiments were performed in triplicates (n = 3). Statistical analysis was performed on the mean values by unpaired comparison of mono-cultured HDF and co-cultured HDF RNA samples by using Student’s t-test (**p<0.01, ***p<0.001; n.s.: not significant). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25919140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pathogen-associated molecular patterns (PAMPs) and damage-associated molecular pattern (DAMPs) induce cytokine expression in human breast cancer cells in vitro. a Relative expression of IL-6 and IL-8 mRNAs after 6 h stimulation with lipopolysaccharide 1 (LPS1) and LPS2, using quantitative real-time PCR (QPCR) on mRNA from the cell lines indicated; n = 6. Error bars standard error of the mean (SEM); ***P <0.001 (analysis of variance (ANOVA)). b Release of IL-6 and IL-8 after stimulation with LPS1 and cycloheximide for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells; n = 3. c-e Relative release of IL-6 and IL-8 after stimulation with LPS1, LPS2, the DAMP HMGB1 or IL-1 beta for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells (c), SUM-149 cells (d) and SUM-159 cells (e); n = 10. Error bars SEM; *P <0.05, ***P <0.001 (ANOVA). f Dual luciferase reporter assays of MDA-MB-231 cells transfected with an NF kappa B reporter. TK-Renilla was co-transfected as control (Ctrl). LPS1, LPS2, HMGB1 or IL-1 beta was added to stimulate NF kappa B activity as described in “Methods”; n = 14. Error bars SEM; ***P <0.001 (ANOVA). g The relative release of IL-6 and IL-8 after stimulation with the DAMP S100A9 for 20 h, using ELISA on supernatants from stimulated MDA-MB-231, SUM-149 and SUM-159 cells; n = 6. Error bars SEM. *P <0.05, ***P <0.001 (ANOVA). h Transient transfection of pDUO-MD2/hTLR4 for 72 h in MCF-7 cells induces release of both IL-6 and IL-8 measured by ELISA; n = 6. Error bars SEM; *P <0.05, ***P <0.001 (Students t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

Toll-like receptor 4 (TLR4) silencing decreases endogenous levels of pro-inflammatory cytokines. a Effect of TLR2/4 silencing in breast cancer cells transfected with negative control (nc) siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2) was analyzed using quantitative real-time PCR; ***P <0.001 (analysis of variance (ANOVA)). b IL-6 (left) and IL-8 (right) ELISA on supernatants from MDA-MB-231 breast cancer cells transfected with nc siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2); n = 4. Error bars standard error of the mean (SEM); *P <0.05, **P <0.01, ***P <0.001 (ANOVA). c Boyden chamber migration assays. Migration of primary human myeloid cells towards supernatants from different cell lines indicated. Human primary peripheral blood mononuclear cells were isolated as previously described [48] and allowed to migrate through a Costar Transwell® Permeable Support 8.0-μm 24-well plate (Corning) to the supernatants of breast cancer supernatants cultured under serum-free conditions. Percentage of migrated CD11b+ cells was analyzed using a flow cytometer and CD11b-APC antibodies (BD Sciences); n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (ANOVA). d Matrigel invasion assays. Invasion of lipopolysaccharide (LPS)-stimulated/un-stimulated MDA-MB-231 cells into matrigel invasion chambers (BD Sciences) as indicated: 25 × 103 MDA-MB-231 cells were stimulated or not with LPS and allowed to invade from 72 h. Amount of invaded cells was analyzed using crystal violet staining and manual counting in four separate experiments; n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (Student’s t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PD8000C
HRP	S8000C

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AF332

CD117/c-kit Antibody [Unconjugated]

(1 Review)

(18 Publications)

100 μg

AF332

Recombinant Human SCF/c‑kit Ligand (Catalog # 255-SC) induces cell proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited bySCF/c‑kit Ligand is neutralized (green line) by increasing concentrations of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332). The ND50 is typically 0.06-0.36 μg/mL in the presence of 20 ng/mL of Recombinant HumanSCF/c‑kit Ligand.

CD117/c-kit was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of MO7e human megakaryocytic leukemic cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD117/c-kit at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 172 kDa (as indicated) using 5 µg/mL of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Tissue fixed in 4% PFA for 1 hour, and cryoprotected in 30% sucrose for 24 hours. Tissue frozen in OCT compound and cut at 10um thickness. Blocked for 1 hour in protein block and stained at 5 ug/mL. Image from a verified customer review.

The protein loading of KIT‐EVs shifts among vesicle subtypes after cell treatment with SMase inhibitors. (A) Viability assessment of HMC‐1.1 cells at the end of the 16 h treatment with DMSO (vehicle control), 10 μM GW4869 (n‐SMase inhibitor) or 10 μM amitriptyline (a‐SMase inhibitor) to control for potential drug‐induced cell death. Data are presented as mean ± S.D. from three independent experiments, normalized to the control. (B, C) EVs released by control‐ or inhibitor‐treated HMC‐1.1 cells were pelleted as P15 (B) or P120 (C) EVs and evaluated by nanoparticle tracking. The sum of P15 (B) or P120 (C) EVs (ranging from 0 to 250 nm) released under each condition was plotted. Representative data are shown as mean ± S.D. from five repetitions. Ns, not significant; **, 0.0011; ****p < 0.0001 (two‐tailed, unpaired Student t‐test). (D) Total lysates (30 μg) of DMSO‐ or inhibitor‐treated cells were analyzed by immunoblotting with the indicated antibodies. (E,F) KIT‐EVs were captured with an antibody against N‐terminal KIT from P15 (3.4 × 108 EVs) (E) or P120 (2.6 × 109 EVs) (F) vesicles released by control, GW4869‐ or amitriptyline‐treated cells. Total KIT‐EV lysates were evaluated by Western blotting; representative blots are shown. (G,H) Signal intensities of proteins in KIT‐EVs (E,F) were quantified and normalized to the DMSO control. Up‐ or down‐regulation (indicated by arrows) of protein content in P15 and P120 KIT‐EVs in response to GW4869 (G) or amitriptyline (H) is displayed in Volcano graphs (n = 3). The q‐value is the FDR‐adjusted p‐value, and the dashed line indicates the significance threshold. In D, E and F, dashed lines imply lanes from the same blot that were not loaded contiguously Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.

$HMC‐1‐derived EVs contain KIT and canonical EV protein markers. (A, B) Detection of canonical EV marker (A) and mast cell marker proteins (B) as well as calreticulin as an exclusion marker in HMC‐1.1 and HMC‐1.2 cells and secreted EVs. 30 μg of protein of cell or EV lysates were separated by SDS‐PAGE, blotted and probed with the indicated antibodies. (C) HMC‐1.1‐derived EVs were separated from soluble protein by size exclusion chromatography (SEC; qEVoriginal70) and eluted as pooled fractions F7‐F11, followed by filter concentration. Cell (30 μg) and EV lysates (15 μg) were analyzed by immunoblotting with the indicated antibodies. The dashed line indicates the samples were run on the same blot, but not in contiguous lanes. (D) A representative graph showing the concentration and size measurement of SEC‐isolated EVs (C) by nanoparticle tracking. The average profile of five workflow repetitions is displayed. (E) Workflow of the immunocapture of EV subpopulations. EVs are bulk‐isolated from pre‐cleared cell culture media by PEG‐precipitation and incubated with antibody‐conjugated beads allowing for immunocapture of specific EV subpopulations. (F) Representative Western blot of CD9‐, CD81‐ and CD63‐immunocaptured EVs. Each bead capture was performed from an equal number of HMC‐1.1‐derived EVs (1.9 × 1010 EVs). Unconjugated beads served as a negative control. The presence of canonical EV protein markers as well as KIT in the EV subpopulations were determined by immunoblotting. *, signal from previous probing with a flotillin‐1 antibody Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$KIT‐containing EVs can be isolated by immunocapture. (B) Representative immunoblots showing the canonical EV marker content of KIT‐EVs captured from HMC‐1.1‐derived EVs that isolated by size exclusion chromatography (SEC). Three different antibodies directed against N‐t, C‐t (left panel) or full‐length (fl) KIT protein (right panel) used for immunocapturing of KIT‐containing EVs. Each pulldown was performed from vesicles secreted by 20 × 106 & collected in SEC fractions seven to nine. Unbound EVs in the flow‐through (FT) pelleted by ultracentrifugation (UC) to demonstrate the capture efficiency. Beads conjugated to isotype control antibodies served as negative controls. Cell lysates (30 μg) included. g, goat; rb, rabbit; m, mouse. Dashed lines imply lanes from the same blot that not loaded contiguously. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$HMC‐1‐derived EVs contain KIT and canonical EV protein markers. (A, B) Detection of canonical EV marker (A) and mast cell marker proteins (B) as well as calreticulin as an exclusion marker in HMC‐1.1 and HMC‐1.2 cells and secreted EVs. 30 μg of protein of cell or EV lysates were separated by SDS‐PAGE, blotted and probed with the indicated antibodies. (C) HMC‐1.1‐derived EVs were separated from soluble protein by size exclusion chromatography (SEC; qEVoriginal70) and eluted as pooled fractions F7‐F11, followed by filter concentration. Cell (30 μg) and EV lysates (15 μg) were analyzed by immunoblotting with the indicated antibodies. The dashed line indicates the samples were run on the same blot, but not in contiguous lanes. (D) A representative graph showing the concentration and size measurement of SEC‐isolated EVs (C) by nanoparticle tracking. The average profile of five workflow repetitions is displayed. (E) Workflow of the immunocapture of EV subpopulations. EVs are bulk‐isolated from pre‐cleared cell culture media by PEG‐precipitation and incubated with antibody‐conjugated beads allowing for immunocapture of specific EV subpopulations. (F) Representative Western blot of CD9‐, CD81‐ and CD63‐immunocaptured EVs. Each bead capture was performed from an equal number of HMC‐1.1‐derived EVs (1.9 × 1010 EVs). Unconjugated beads served as a negative control. The presence of canonical EV protein markers as well as KIT in the EV subpopulations were determined by immunoblotting. *, signal from previous probing with a flotillin‐1 antibody Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$KIT‐EVs are released as heterogeneous subpopulations with different physical properties. (A) Cell culture supernatant was subjected to differential ultracentrifugation to obtain P15 and P120 EV pellets, as illustrated. Where indicated, P15 and P120 EVs were further separated by iodixanol density gradients. Vesicles from each density fraction were pelleted and analyzed. (B) P15 and P120 EVs obtained from HMC‐1.1 cells were analyzed by nanoparticle tracking. Representative concentration and size profiles averaged from five workflow repetitions are depicted. The averaged percentage (%) of different size ranges within the P15 or P120 population is shown on top (bar chart). (C) Representative Western blot of HMC‐1.1 cell, P15 and P120 EV lysates (30 μg) probed with the indicated antibodies. (D, E) HMC‐1.1‐derived P15 (D) or P120 (E) EV pellets were separated by density gradients and the vesicles from each fraction (F1‐F10) were analyzed by nanoparticle tracking for concentration (particles/ml), shown as box plots, and size (represented as average mode, nm), shown as a line graph. Data in the box plots represent the median with the whiskers indicating minimum and maximum values; data in the line graph are represented as mean ± S.D. from five repetitions. Representative results are shown. (F, G) Representative immunoblots showing the presence of KIT and annexin A1 in P15 EV fractions (F) and KIT and CD81 in P120 EV fractions (G). The density (g/ml) of each fraction is indicated below Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

KIT‐EV immunocapture of LAD2 huMC EVs and KIT‐EV recovery from spiked plasma. (A) Cytokine‐starved LAD2 huMCs were treated or not with stem cell factor (SCF; 100 ng/ml) for 2 h in HEPES‐BSA buffer. Cell lysates (30 μg) were analyzed by immunoblotting with the indicated antibodies. (B) EVs released into the supernatant by 18 × 106 LAD2 cells treated or not with SCF for 2 h were sedimented by differential ultracentrifugation into P15 and P120 EVs. KIT‐EV immunocapture was performed (KIT antibody AF332) from all sedimented LAD2 P15 and P120 EVs. The total bead elution was loaded and analyzed by Western blotting with the indicated antibodies. Input EV lysates (5 × 108) were included. Representative blots are shown. (C) HMC‐1.1 cell line‐derived EVs (1.2 × 1010 EVs) were incubated or not in human normal plasma and captured with a KIT antibody (AF332) or by CD9 antibody‐conjugated beads. A goat HA antibody served as a negative isotype control. Representative blots are shown. sKIT, soluble KIT that is present in plasma. Dashed lines imply lanes from the same blot that were not loaded contiguously Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.

$HMC‐1‐derived EVs contain KIT and canonical EV protein markers. (A, B) Detection of canonical EV marker (A) and mast cell marker proteins (B) as well as calreticulin as an exclusion marker in HMC‐1.1 and HMC‐1.2 cells and secreted EVs. 30 μg of protein of cell or EV lysates were separated by SDS‐PAGE, blotted and probed with the indicated antibodies. (C) HMC‐1.1‐derived EVs were separated from soluble protein by size exclusion chromatography (SEC; qEVoriginal70) and eluted as pooled fractions F7‐F11, followed by filter concentration. Cell (30 μg) and EV lysates (15 μg) were analyzed by immunoblotting with the indicated antibodies. The dashed line indicates the samples were run on the same blot, but not in contiguous lanes. (D) A representative graph showing the concentration and size measurement of SEC‐isolated EVs (C) by nanoparticle tracking. The average profile of five workflow repetitions is displayed. (E) Workflow of the immunocapture of EV subpopulations. EVs are bulk‐isolated from pre‐cleared cell culture media by PEG‐precipitation and incubated with antibody‐conjugated beads allowing for immunocapture of specific EV subpopulations. (F) Representative Western blot of CD9‐, CD81‐ and CD63‐immunocaptured EVs. Each bead capture was performed from an equal number of HMC‐1.1‐derived EVs (1.9 × 1010 EVs). Unconjugated beads served as a negative control. The presence of canonical EV protein markers as well as KIT in the EV subpopulations were determined by immunoblotting. *, signal from previous probing with a flotillin‐1 antibody Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

KIT‐containing EVs can be isolated by immunocapture. (A) Illustration of the predicted topology of the receptor KIT in an EV. KIT is embedded in the EV membrane by its transmembrane domain (TMD) with the N‐terminal (N‐t) ligand‐binding domain exposed to the extravesicular environment, while the C‐terminal (C‐t) protein domain is directed into the EV lumen. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.

$KIT‐EVs are released as heterogeneous subpopulations with different physical properties. (A) Cell culture supernatant was subjected to differential ultracentrifugation to obtain P15 and P120 EV pellets, as illustrated. Where indicated, P15 and P120 EVs were further separated by iodixanol density gradients. Vesicles from each density fraction were pelleted and analyzed. (B) P15 and P120 EVs obtained from HMC‐1.1 cells were analyzed by nanoparticle tracking. Representative concentration and size profiles averaged from five workflow repetitions are depicted. The averaged percentage (%) of different size ranges within the P15 or P120 population is shown on top (bar chart). (C) Representative Western blot of HMC‐1.1 cell, P15 and P120 EV lysates (30 μg) probed with the indicated antibodies. (D, E) HMC‐1.1‐derived P15 (D) or P120 (E) EV pellets were separated by density gradients and the vesicles from each fraction (F1‐F10) were analyzed by nanoparticle tracking for concentration (particles/ml), shown as box plots, and size (represented as average mode, nm), shown as a line graph. Data in the box plots represent the median with the whiskers indicating minimum and maximum values; data in the line graph are represented as mean ± S.D. from five repetitions. Representative results are shown. (F, G) Representative immunoblots showing the presence of KIT and annexin A1 in P15 EV fractions (F) and KIT and CD81 in P120 EV fractions (G). The density (g/ml) of each fraction is indicated below Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$KIT‐containing EVs can be isolated by immunocapture. (B) Representative immunoblots showing the canonical EV marker content of KIT‐EVs captured from HMC‐1.1‐derived EVs that isolated by size exclusion chromatography (SEC). Three different antibodies directed against N‐t, C‐t (left panel) or full‐length (fl) KIT protein (right panel) used for immunocapturing of KIT‐containing EVs. Each pulldown was performed from vesicles secreted by 20 × 106 & collected in SEC fractions seven to nine. Unbound EVs in the flow‐through (FT) pelleted by ultracentrifugation (UC) to demonstrate the capture efficiency. Beads conjugated to isotype control antibodies served as negative controls. Cell lysates (30 μg) included. g, goat; rb, rabbit; m, mouse. Dashed lines imply lanes from the same blot that not loaded contiguously. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$KIT‐EVs are released as heterogeneous subpopulations with different physical properties. (A) Cell culture supernatant was subjected to differential ultracentrifugation to obtain P15 and P120 EV pellets, as illustrated. Where indicated, P15 and P120 EVs were further separated by iodixanol density gradients. Vesicles from each density fraction were pelleted and analyzed. (B) P15 and P120 EVs obtained from HMC‐1.1 cells were analyzed by nanoparticle tracking. Representative concentration and size profiles averaged from five workflow repetitions are depicted. The averaged percentage (%) of different size ranges within the P15 or P120 population is shown on top (bar chart). (C) Representative Western blot of HMC‐1.1 cell, P15 and P120 EV lysates (30 μg) probed with the indicated antibodies. (D, E) HMC‐1.1‐derived P15 (D) or P120 (E) EV pellets were separated by density gradients and the vesicles from each fraction (F1‐F10) were analyzed by nanoparticle tracking for concentration (particles/ml), shown as box plots, and size (represented as average mode, nm), shown as a line graph. Data in the box plots represent the median with the whiskers indicating minimum and maximum values; data in the line graph are represented as mean ± S.D. from five repetitions. Representative results are shown. (F, G) Representative immunoblots showing the presence of KIT and annexin A1 in P15 EV fractions (F) and KIT and CD81 in P120 EV fractions (G). The density (g/ml) of each fraction is indicated below Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36239715), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF332U-100UG
Alexa Fluor 405	AF332V-100UG
Alexa Fluor 488	AF332G-100UG
Alexa Fluor 532	AF332X-100UG
Alexa Fluor 594	AF332T-100UG
Alexa Fluor 647	AF332R-100UG
Alexa Fluor 700	AF332N-100UG
Alexa Fluor 750	AF332S-100UG
Allophycocyanin	AF332A
Biotin	BAF332
CoraFluor 1	AF332CL1
DyLight 350	AF332D
DyLight 405	AF332E
DyLight 488	AF332K
DyLight 550	AF332L
DyLight 594	AF332M
DyLight 650	AF332W
DyLight 680	AF332Y
DyLight 755	AF332Z
FITC	AF332F
HRP	AF332H
Janelia Fluor 525	AF332JF525
Janelia Fluor 549	AF332I
Janelia Fluor 646	AF332J
Janelia Fluor 669	AF332JF669
PE	AF332P
PerCP	AF332C
mFluor Violet 450 SE	AF332MFV450
mFluor Violet 500 SE	AF332MFV500
mFluor Violet 610 SE	AF332MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF332-SP

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MCK00

Mouse SCF Quantikine ELISA Kit

(27 Publications)

1 Kit

MCK00

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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DCK00

Human SCF Quantikine ELISA Kit

(25 Publications)

1 Kit

DCK00

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NB100-77477

CD117/c-kit Antibody (2B8) - BSA Free

(1 Review)

(11 Publications)

0.1 mg

NB100-77477

Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.

Identification of c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and (a) Kit-129 aptamer or (b) rabbit anti-c-kit antibody 2B8.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-77477AF350
Alexa Fluor 532	NB100-77477AF532
Alexa Fluor 594	NB100-77477AF594
Alexa Fluor 750	NB100-77477AF750
Allophycocyanin	NB100-77477APC-0.1ml
Allophycocyanin/Cy7	NB100-77477APCCY7
Biotin	NB100-77477B
CoraFluor 1	NB100-77477CL1
DyLight 350	NB100-77477UV-0.1ml
DyLight 405	NB100-77477V-0.1ml
DyLight 488	NB100-77477G
DyLight 550	NB100-77477R
DyLight 594	NB100-77477DL594
DyLight 650	NB100-77477C
DyLight 680	NB100-77477FR-0.1ml
DyLight 755	NB100-77477IR-0.1ml
FITC	NB100-77477F
HRP	NB100-77477H
Janelia Fluor 525	NB100-77477JF525
Janelia Fluor 549	NB100-77477JF549
Janelia Fluor 646	NB100-77477JF646
Janelia Fluor 669	NB100-77477JF669
PE	NB100-77477PE-0.1ml
PE/Atto594	NB100-77477PEATT594
PE/Cy5.5	NB100-77477PECY55
PE/Cy7	NB100-77477PECY7
PerCP	NB100-77477PCP-0.1ml
mFluor Violet 450 SE	NB100-77477MFV450
mFluor Violet 500 SE	NB100-77477MFV500
mFluor Violet 610 SE	NB100-77477MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-77477SS
Azide and BSA Free	NBP2-80624

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NBP1-43359

CD117/c-kit Antibody (ACK2) - BSA Free

(1 Review)

(9 Publications)

0.1 mg

NBP1-43359

Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were

Reactivity:

Hu, Mu, Gp

Species Glossary

Reactivity: Hu, Mu, Gp

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43359AF350
Alexa Fluor 405	NBP1-43359AF405
Alexa Fluor 488	NBP1-43359AF488
Alexa Fluor 532	NBP1-43359AF532
Alexa Fluor 594	NBP1-43359AF594
Alexa Fluor 647	NBP1-43359AF647
Alexa Fluor 700	NBP1-43359AF700
Alexa Fluor 750	NBP1-43359AF750
Allophycocyanin	NBP1-43359APC
Allophycocyanin/Cy7	NBP1-43359APCCY7
Biotin	NBP1-43359B
CoraFluor 1	NBP1-43359CL1
DyLight 350	NBP1-43359UV
DyLight 405	NBP1-43359V
DyLight 488	NBP1-43359G
DyLight 550	NBP1-43359R
DyLight 594	NBP1-43359DL594
DyLight 650	NBP1-43359C
DyLight 680	NBP1-43359FR
DyLight 755	NBP1-43359IR
FITC	NBP1-43359F
HRP	NBP1-43359H
Janelia Fluor 525	NBP1-43359JF525
Janelia Fluor 549	NBP1-43359JF549
Janelia Fluor 646	NBP1-43359JF646
Janelia Fluor 669	NBP1-43359JF669
PE	NBP1-43359PE
PE/Atto594	NBP1-43359PEATT594
PE/Cy5.5	NBP1-43359PECY55
PE/Cy7	NBP1-43359PECY7
PerCP	NBP1-43359PCP
mFluor Violet 450 SE	NBP1-43359MFV450
mFluor Violet 500 SE	NBP1-43359MFV500
mFluor Violet 610 SE	NBP1-43359MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43359-0.05mg
BSA Free	NBP1-43359-0.025mg

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MAB1356

CD117/c-kit Antibody (180627) [Unconjugated]

(11 Publications)

100 μg

MAB1356

CD117/c-kit was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # MAB1356) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to Bergmann glial cells located around Purkinje cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1356U-100UG
Alexa Fluor 405	FAB1356V-100UG
Alexa Fluor 488	FAB1356G-100UG
Alexa Fluor 532	FAB1356X
Alexa Fluor 594	FAB1356T-100UG
Alexa Fluor 647	FAB1356R-100UG
Alexa Fluor 700	FAB1356N-100UG
Alexa Fluor 750	FAB1356S-100UG
Allophycocyanin	FAB1356A
Allophycocyanin/Cy7	FAB1356APCCY7
Biotin	BAM1356
CoraFluor 1	FAB1356CL1
DyLight 350	FAB1356D
DyLight 405	FAB1356E
DyLight 488	FAB1356K
DyLight 550	FAB1356L
DyLight 594	FAB1356M
DyLight 650	FAB1356W
DyLight 680	FAB1356Y
DyLight 755	FAB1356Z
FITC	FAB1356F
HRP	FAB1356H
Janelia Fluor 525	FAB1356JF525
Janelia Fluor 549	FAB1356I
Janelia Fluor 646	FAB1356J
Janelia Fluor 669	FAB1356JF669
PE/Atto594	FAB1356PEATT594
PE/Cy5.5	FAB1356PECY55
PE/Cy7	FAB1356PECY7
PerCP	FAB1356C
Phycoerythrin	FAB1356P-025
Phycoerythrin	FAB1356P-100
mFluor Violet 450 SE	FAB1356MFV450
mFluor Violet 500 SE	FAB1356MFV500
mFluor Violet 610 SE	FAB1356MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1356A
Carrier Free	MAB1356-SP
Carrier Free	MAB1356-500
Carrier Free	FAB1356P-100
Carrier Free	FAB1356P-025
Carrier Free	FAB1356V-100UG
Carrier Free	FAB1356U-100UG
Carrier Free	FAB1356T-100UG
Carrier Free	FAB1356S-100UG
Carrier Free	FAB1356R-100UG
Carrier Free	FAB1356N-100UG
Carrier Free	FAB1356G-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Recombinant Human SCF Protein

Recombinant Mouse SCF Protein

CD117/c-kit Antibody [Unconjugated]

Human TNF-alpha Quantikine ELISA Kit

Human IL-8/CXCL8 DuoSet ELISA, 15 Plate

Human IL-8/CXCL8 Quantikine ELISA Kit

CD117/c-kit Antibody [Unconjugated]

Mouse SCF Quantikine ELISA Kit

Human SCF Quantikine ELISA Kit

CD117/c-kit Antibody (2B8) - BSA Free

CD117/c-kit Antibody (ACK2) - BSA Free

CD117/c-kit Antibody (180627) [Unconjugated]