Recombinant Mouse SCF Protein

Images

 
1 μg/lane of Recombinant Mouse SCF/c-kit was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 2.5-10 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse SCF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 2.5-10 ng/mL.
Source
E. coli-derived mouse SCF/c-kit Ligand protein
Lys26-Ala189, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Kitl
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
455-MC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SCF Protein

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 - 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

  1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
  2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
  5. Kapur, R. et al. (2002) Blood 100:1287.
  6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
  8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
  9. Huang, E. et al. (1990) Cell 63:225.
  10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
  11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
  12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
  13. Flanagan, J.G. et al. (1991) Cell 64:1025.
  14. Martin, F.H. et al. (1990) Cell 63:203.
  15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

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Publications for SCF/c-kit Ligand (455-MC)(163)

We have publications tested in 5 confirmed species: Human, Mouse, Chicken, Rabbit, Transgenic Mouse.

We have publications tested in 6 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Multiplex Standard, cell culture media.


Filter By Application
Bioassay
(140)
Cell Culture
(11)
Differentiation
(1)
In Vivo
(7)
Multiplex Standard
(1)
cell culture media
(1)
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Human
(4)
Mouse
(151)
Chicken
(2)
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(2)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 163. Show All 163 Publications.
Publications using 455-MC Applications Species
R Deshycka, V Sudaryo, NJ Huang, Y Xie, LY Smeding, MK Choi, HL Ploegh, HF Lodish, N Pishesha Engineered red blood cells carrying PCSK9 inhibitors persistently lower LDL and prevent obesity PLoS ONE, 2021;16(11):e0259353. 2021 [PMID: 34731223] (Bioassay, Mouse) Bioassay Mouse
T Kobayashi, A Castillo-V, CA Penfold, M Morgan, N Mizuno, WWC Tang, Y Osada, M Hirao, F Yoshida, H Sato, H Nakauchi, M Hirabayash, MA Surani Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells Cell Reports, 2021;37(2):109812. 2021 [PMID: 34644585] (Bioassay, Rabbit) Bioassay Rabbit
HZ Guo, ZH Guo, SH Yu, LT Niu, WT Qiang, MM Huang, YY Tian, J Chen, H Yang, XQ Weng, Y Zhang, W Zhang, SY Hu, J Shi, J Zhu Leukemic progenitor cells enable immunosuppression and post-chemotherapy relapse via IL-36-inflammatory monocyte axis Science Advances, 2021;7(41):eabg4167. 2021 [PMID: 34623912] (Bioassay, Mouse) Bioassay Mouse
SR Naidu, M Capitano, J Ropa, S Cooper, X Huang, HE Broxmeyer Chromatin remodeling subunit BRM and valine regulate hematopoietic stem/progenitor cell function and self-renewal via intrinsic and extrinsic effects Leukemia, 2021;0(0):. 2021 [PMID: 34599272] (Bioassay, Mouse) Bioassay Mouse
Y Ishikura, H Ohta, T Sato, Y Murase, Y Yabuta, Y Kojima, C Yamashiro, T Nakamura, T Yamamoto, T Ogawa, M Saitou In�vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34496297] (Bioassay, Mouse) Bioassay Mouse
C Zhao, Y Zhao, J Zhao, G Meng, S Huang, Y Liu, S Wang, L Qi Acute myeloid leukemia cell-derived extracellular vesicles carrying microRNA-548ac regulate hematopoietic function via the TRIM28/STAT3 pathway Cancer Gene Therapy, 2021;0(0):. 2021 [PMID: 34453123] (Bioassay, Mouse) Bioassay Mouse
K Yamamoto, S Goyama, S Asada, T Fujino, T Yonezawa, N Sato, R Takeda, A Tsuchiya, T Fukuyama, Y Tanaka, A Yokoyama, H Toya, A Kon, Y Nannya, R Onoguchi-M, S Nakagawa, T Hirose, S Ogawa, N Akimitsu, T Kitamura A histone modifier, ASXL1, interacts with NONO and is involved in paraspeckle formation in hematopoietic cells Cell Reports, 2021;36(8):109576. 2021 [PMID: 34433054] (Bioassay, Mouse) Bioassay Mouse
N Ohashi, T Terashima, M Katagi, Y Nakae, J Okano, Y Suzuki, H Kojima GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS Scientific Reports, 2021;11(1):12803. 2021 [PMID: 34140581] (Cell Culture, Mouse) Cell Culture Mouse
F Herbst, TJL Lang, ESP Eckert, P Wünsche, AA Wurm, T Kindinger, K Laaber, S Hemmati, A Hotz-Wagen, O Zavidij, A Paruzynski, J Lu, C von Kalle, T Zenz, C Klein, M Schmidt, CR Ball, H Glimm The balance between the intronic miR-342 and its host gene Evl determines hematopoietic cell fate decision Leukemia, 2021;0(0):. 2021 [PMID: 34021250] (Bioassay, Mouse) Bioassay Mouse
RA Cardenas, R Gonzalez, E Sanchez, MA Ramos, EI Cardenas, AI Rodarte, RJ Alcazar-Fe, A Isaza, AR Burns, R Heidelberg, R Adachi SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis The Journal of Biological Chemistry, 2021;296(0):100268. 2021 [PMID: 33837726] (Cell Culture, Mouse) Cell Culture Mouse
Show All 163 Publications.

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Bioinformatics

Gene Symbol Kitl
Uniprot