7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL." class="big_lightbox"> 7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL." alt="Recombinant human TGF-beta 1 (7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL." class="big_thumb" />

7754-BH) Recombinant Human TGF‑ beta 1 as measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cell. (orange, green, respectively)." class="big_lightbox"> 7754-BH) Recombinant Human TGF‑ beta 1 as measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cell. (orange, green, respectively)." alt="Equivalent bioactivity of CHO-derived (Catalog # 240-B) and HEK293-derived (7754-BH) Recombinant Human TGF‑ beta 1 as measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cell. (orange, green, respectively)." class="big_thumb" />

7666-MB) inhibits IL-4-dependent proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL." class="big_lightbox"> 7666-MB) inhibits IL-4-dependent proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL." alt="Recombinant mouse TGF-beta 1 (7666-MB) inhibits IL-4-dependent proliferation in the HT-2 mouse T cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL." class="big_thumb" />

240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_lightbox"> 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835). The ND₅₀ is typically 0.25-1.25 µg/mL." alt="Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_thumb" />

240-B) was serially diluted and captured by Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835) coated on a Clear Polystyrene Microplate (Catalog # DY990). Chicken Anti-TGF‑ beta 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 240-B) was serially diluted and captured by Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835) coated on a Clear Polystyrene Microplate (Catalog # DY990). Chicken Anti-TGF‑ beta 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epitjhelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epitjhelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="TGF-beta was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Rabbit Anti-TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epitjhelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

101-B1) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA). The ND₅₀range is 5-30 µg/mL." class="big_lightbox"> 101-B1) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA). The ND₅₀range is 5-30 µg/mL." class="big_thumb" />

HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

246-LP) inhibits Recom-binant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombin-ant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_lightbox"> 246-LP) inhibits Recom-binant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombin-ant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_thumb" />

8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta 3 (Catalog # 243-B3). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human TGF‑ beta 3 proteins are measured by their ability to inhibit the IL-4-dependent proliferation of Ht-2 mouse T cell. (orange, green, respectively). " class="big_lightbox"> 8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta 3 (Catalog # 243-B3). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human TGF‑ beta 3 proteins are measured by their ability to inhibit the IL-4-dependent proliferation of Ht-2 mouse T cell. (orange, green, respectively). " alt="As an alternative, please consider our next generation CHO-drived Recombinant Human TGF‑ beta 3 (8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta 3 (Catalog # 243-B3). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human TGF‑ beta 3 proteins are measured by their ability to inhibit the IL-4-dependent proliferation of Ht-2 mouse T cell. (orange, green, respectively). " class="big_thumb" />

NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TGF-beta 1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (0.25 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (125 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_lightbox"> 246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (0.25 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (125 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />