ZNRF3 Antibody (2035A) [Unconjugated]

ZNRF3 was detected in immersion fixed HEK human embryonic kidney cell transfectants using Rabbit Anti-Mouse ZNRF3 Monoclonal Antibody (Catalog # MAB8328) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 2035A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ZNRF3 Antibody (2035A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ZNRF3
  Lys53-Met216
  Accession # Q5SSZ7
• Specificity: Detects mouse ZNRF3 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZNRF3 Antibody (2035A) [Unconjugated]

• BK747E2.3
• KIAA1133
• novel C3HC4 type Zinc finger (ring finger)
• RNF203
• zinc and ring finger 3
• zinc/RING finger protein 3
• ZNRF3

Background

ZNRF3 (Zinc/RING Finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).

1. de Lau, W. et al. (2014) Genes Dev. 28:305.
2. Xie, Y. et al. (2013) EMBO Rep. 14:1120.
3. Peng, W.C. et al. (2013) PLoS One 8:e83110.
4. Hao, H.X. et al. (2012) Nature 485:195.
5. Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
6. Koo, B.K. et al. (2012) Nature 488:665.
7. Ong, C.K. et al. (2012) Nat. Genet. 44:690.
8. Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
9. Ryland, G.L. et al. (2013) J. Pathol. 229:469.
10. Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q5SSZ7()