ZNF704 Antibody (PCRP-ZNF704-3C10) - Azide and BSA Free

Protein Array: ZNF704 Antibody (PCRP-ZNF704-3C10) - Azide and BSA Free [NBP3-14168] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ZNF704 Antibody (PCRP-ZNF704-3C10).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ZNF704 antibody (PCRP-ZNF704-3C10). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ZNF704 Antibody (PCRP-ZNF704-3C10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640 phalloidin (red).

Flow cytometric analysis of PFA-fixed HeLa cells. ZNF704 Antibody (PCRP-ZNF704-3C10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, IP, MA
• Clone: PCRP-ZNF704-3C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

ZNF704 Antibody (PCRP-ZNF704-3C10) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13765).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human ZNF704 protein (Uniprot: Q6ZNC4)
• Localization: Nucleus
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF704
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 45.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for ZNF704 Antibody (PCRP-ZNF704-3C10) - Azide and BSA Free

• FLJ16218
• MGC10858
• zinc finger protein 704

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF704