ZNF536 Antibody

Images

 
Western Blot: ZNF536 Antibody [NBP1-77092] - Human brain tissue lysate with ZNF536 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: ZNF536 Antibody [NBP1-77092] - Immunofluorescence of ZNF536 in human brain tissue with ZNF536 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: ZNF536 Antibody [NBP1-77092] - Human brain tissue with ZNF536 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ZNF536 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ZNF536. The immunogen is located within amino acids 30 - 80 of ZNF536.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF536
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
ZNF536 Peptide (NBP1-77092PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZNF536 Antibody

  • KIAA0390
  • zinc finger protein 536

Background

ZNF536 is a recently identified zinc-finger protein that is expressed primarily in the developing nervous system and the cerebral cortex, hippocampus, and hypothalamus. ZNF536 possess ten zinc fingers and interacts with CtBP1, a corepressor for gene transcription. It is most closely related to transcriptional repressor ZNF219. Overexpression of ZNF536 in embryonic stem cells dramatically reduced the mRNA levels of neuronal marker genes such as Pax6, MAP2, and beta-tubulin III following retinoic acid (RA)-induced differentiation, while depletion of ZNF536 via RNAi resulted in elevated mRNA levels of these genes, indicating its role in inhibiting neuronal cell differentiation. Overexpression of RA receptor a rescues the inhibitory role of ZNF536, suggesting that ZNF536 might inhibit RA response element-mediated transcriptional activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZNF536 Antibody (NBP1-77092) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for ZNF536 Antibody (NBP1-77092)

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Bioinformatics

Gene Symbol ZNF536
Uniprot