ZNF536 Antibody - BSA Free

Western Blot: ZNF536 Antibody [NBP1-77092] - Human brain tissue lysate with ZNF536 antibody at 1 ug/mL.

Immunohistochemistry: ZNF536 Antibody - BSA Free [NBP1-77092] - Immunohistochemistry of ZNF536 in human brain tissue with ZNF536 antibody at 5 u/mL.

Immunocytochemistry/ Immunofluorescence: ZNF536 Antibody - BSA Free [NBP1-77092] - Immunofluorescence of ZNF536 in human brain tissue with ZNF536 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZNF536 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZNF536 Antibody - BSA Free (NBP1-77092) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ZNF536. The immunogen is located within amino acids 30 - 80 of ZNF536. Amino Acid Squence: PELHPRPNPEEKPPAS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF536
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  ZNF536 Peptide (NBP1-77092PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZNF536 Antibody - BSA Free

• KIAA0390
• zinc finger protein 536

Background

ZNF536 is a recently identified zinc-finger protein that is expressed primarily in the developing nervous system and the cerebral cortex, hippocampus, and hypothalamus. ZNF536 possess ten zinc fingers and interacts with CtBP1, a corepressor for gene transcription. It is most closely related to transcriptional repressor ZNF219. Overexpression of ZNF536 in embryonic stem cells dramatically reduced the mRNA levels of neuronal marker genes such as Pax6, MAP2, and beta-tubulin III following retinoic acid (RA)-induced differentiation, while depletion of ZNF536 via RNAi resulted in elevated mRNA levels of these genes, indicating its role in inhibiting neuronal cell differentiation. Overexpression of RA receptor a rescues the inhibitory role of ZNF536, suggesting that ZNF536 might inhibit RA response element-mediated transcriptional activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF536
• Uniprot:
  • NP_055532(Human)