ZNF462 Antibody - BSA Free

Western Blot: ZNF462 Antibody [NBP1-78192] - Detection of Human ZNF462 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody NBP1-78192 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: ZNF462 Antibody [NBP1-78192] - Section of human lung cancer. Antibody: Affinity purified rabbit anti- ZNF462 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.

Western Blot: ZNF462 Antibody [NBP1-78192] - NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: ZNF462 Antibody [NBP1-78192] - Detection of human ZNF462 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZNF462 antibody NBP1-78192 (lot NBP1-78192-2) used for IP at 6 ug per reaction. ZNF462 was also immunoprecipitated by a previous lot of this antibody NBP1-78192-1. For blotting immunoprecipitated ZNF462, NBP1-78192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZNF462 Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 2456 and 2506 of human Zinc Finger Protein 462 using the numbering given in entry NP_067047.4 (GeneID 58499).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF462
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 -1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-10 mcg/mg
  • Western Blot 1:1000-1:5000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF462 Antibody - BSA Free

• BAZ 1
• BAZ1
• BAZ-1
• BWSCR2-associated zinc finger protein 1
• zinc finger protein 214

Background

ZNF462 may be involved in transcriptional regulation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF462
• Uniprot:
  • NP_067047.4(Human)