ZNF281 Antibody (801706) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 801706
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

ZNF281 Antibody (801706) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human ZNF281
  Asn681-Arg895
  Accession # Q9Y2X9
• Specificity: Detects human ZNF281 in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZNF281 Antibody (801706) [Alexa Fluor® 700]

• FLJ20557
• GZP1
• ZBP-99
• zinc finger protein 416
• ZNF281
• ZNP-99

Background

ZNF281 (Zinc Finger protein 28), also known as ZBP99 and GZP1, is a 100 kDa member of the Krueppel C2H2-type Zn-finger family of proteins. It is found in embryonic stem cells, placenta, and lymphocytes, and appears to be necessary for the maintenance of pluripotency in stem cells. ZNF281 both represses (STAT3) and activates (Nanog) multiple genes, and is known to form a complex with Nanog, Oct4 and Sox2. Human ZFN281 is 895 amino acids in length. It contains one Gly-rich region (aa 4-37), a poly-Pro motif (aa 90-96) and four consecutive C2H2-type zinc finger domains (aa 261-367). There are phosphorylation sites at Ser255, 395, 484, 785 and 807. Potential alternate start sites exist at Met296 and Met434. Over aa 681-895, human ZFN281 shares 99% aa identity with mouse ZFN281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.