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ZNF274 Overexpression Lysate

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Western Blot: ZNF274 Overexpression Lysate (Adult Normal) [NBL1-18107] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZNF274.
Western Blot: ZNF274 Overexpression Lysate (Adult Normal) [NBL1-18107] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZNF274.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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ZNF274 Overexpression Lysate Summary

Description

ZNF274 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC211914

Accession#: NM_133502

Protein Tag: C-MYC/DDK
Gene
ZNF274

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ZNF274 Overexpression Lysate

  • GIOT2
  • GIOT-2
  • gonadotropin inducible transcription repressor-2
  • Gonadotropin-inducible ovary transcription repressor 2
  • gonadotropin-inducible transcription repressor 2
  • KOX7DKFZp434F1811
  • zinc finger protein 44 (KOX 7)
  • zinc finger protein 44
  • Zinc finger protein 55DKFZp686L21136
  • Zinc finger protein 58ZNF504
  • Zinc finger protein KOX7
  • zinc finger protein ZnFP12
  • ZNF
  • ZNF55
  • ZNF58

Background

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq]. Transcript Variant: This variant (ZNF274c) encodes the longest isoform (c). Isoform c contains two KRAB A domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ZNF274