ZNF274 Antibody (1D8) - Azide and BSA Free

Western Blot: ZNF274 Antibody (1D8) [H00010782-M04] - Analysis of ZNF274 expression in transfected 293T cell line by ZNF274 monoclonal antibody (M04), clone 1D8. Lane 1: ZNF274 transfected lysatE (74.2 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ZNF274 Antibody (1D8) [H00010782-M04] - Analysis of monoclonal antibody to ZNF274 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: ZNF274 Antibody (1D8) [H00010782-M04] - Western blot analysis of ZNF274 over-expressed 293 cell line, cotransfected with ZNF274 Validated Chimera RNAi or non-transfected control. Blot probed with H00010782-M04. GAPDH (36.1 kDa) used as loading control.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, Mycoplasma
• Clone: 1D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ZNF274 Antibody (1D8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ZNF274 Antibody (1D8) - Azide and BSA Free (H00010782-M04) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ZNF274 (NP_598009, 420 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHT
• Specificity: ZNF274 - zinc finger protein 274
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF274
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot
• Application Notes: Antibody reactive against recombinant protein on ELISA. It has also been used for RNAi Validation and Western Blot.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZNF274 Antibody (1D8) - Azide and BSA Free

• GIOT2
• GIOT-2
• gonadotropin inducible transcription repressor-2
• Gonadotropin-inducible ovary transcription repressor 2
• gonadotropin-inducible transcription repressor 2
• KOX7DKFZp434F1811
• zinc finger protein 44 (KOX 7)
• zinc finger protein 44
• Zinc finger protein 55DKFZp686L21136
• Zinc finger protein 58ZNF504
• Zinc finger protein KOX7
• zinc finger protein ZnFP12
• ZNF
• ZNF55
• ZNF58

Background

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF274
• Entrez:
  • 10782(Human)
• OMIM:
  • 605467(Human)
• Uniprot:
  • NP_598009(Human)