ZHX2 Antibody (5E2) - Azide and BSA Free

Western Blot: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of ZHX2 over-expressed 293 cell line, cotransfected with ZHX2 Validated Chimera RNAi ( Cat # H00022882-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZHX2 monoclonal antibody (M01), clone 5E2 (Cat # H00022882-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunocytochemistry/Immunofluorescence: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of monoclonal antibody to ZHX2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of monoclonal antibody to ZHX2 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml.

Western Blot: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of ZHX2 expression in transfected 293T cell line by ZHX2 monoclonal antibody (M01), clone 5E2.Lane 1: ZHX2 transfected lysate(92.307 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, Mycoplasma
• Clone: 5E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ZHX2 Antibody (5E2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ZHX2 Antibody (5E2) - Azide and BSA Free (H00022882-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ZHX2 (NP_055758, 691 a.a. ~ 788 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEEDLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEES
• Localization: Nuclear
• Specificity: ZHX2 - zinc fingers and homeoboxes 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZHX2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against recominant protein and transfected lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation, and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZHX2 Antibody (5E2) - Azide and BSA Free

• zona pellucida glycoprotein 2 (sperm receptor)
• Zona pellucida glycoprotein 2
• zona pellucida glycoprotein ZP2
• Zona pellucida protein A
• zona pellucida sperm-binding protein 2
• Zp-2
• ZPA

Background

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZHX2
• Entrez:
  • 22882(Human)
• OMIM:
  • 609185(Human)
• Uniprot:
  • NP_055758(Human)