ZFP200 Antibody (OTI5F4) - Azide and BSA Free

Western Blot: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF200.

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: ZFP200 Antibody (OTI5F4) - Azide and BSA Free [NBP2-74923] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI5F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ZFP200 Antibody (OTI5F4) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ZNF200 (NP_932353) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF200
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 45.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ZFP200 Antibody (OTI5F4) - Azide and BSA Free

• MGC45293
• zinc finger protein 200
• ZNFMF

Background

ZFP200 (ZNF200) is a gene that codes for a protein with three isoforms, with lengths of 395, 394 and 394 amino acids and weights of approximately 46, 45, and 45 kDa respectively. ZFP200 is highly expressed in the testis and could have a role in spermatogenesis. Current studies are being done on diseases and disorder related to this gene including familial Mediterranean fever, anemia, and prostatitis. ZFP200 has also been shown to have interactions with PPARGC1B and UBC in pathways such as the generic transcription and gene expression pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF200