ZBTB46 Antibody (PCRP-ZBTB46-2B8) - Azide and BSA Free

Immunohistochemistry-Paraffin: ZBTB46 Antibody (PCRP-ZBTB46-2B8) [NBP3-08298] - Formalin-fixed, paraffin-embedded human tonsil stained with ZBTB46 Mouse Monoclonal Antibody (PCRP-ZBTB46-2B8).

Flow Cytometry: ZBTB46 Antibody (PCRP-ZBTB46-2B8) [NBP3-08298] - Flow Cytometric Analysis of PFA-fixed HeLa cells. ZBTB46 Mouse Monoclonal Antibody (PCRP-ZBTB46-2B8) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: ZBTB46 Antibody (PCRP-ZBTB46-2B8) [NBP3-08298] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ZBTB46 Mouse Monoclonal Antibody (PCRP-ZBTB46-2B8).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, MA
• Clone: PCRP-ZBTB46-2B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

ZBTB46 Antibody (PCRP-ZBTB46-2B8) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07755).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human ZBTB46 protein (Uniprot: Q86UZ6)
• Localization: Nucleus
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZBTB46
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for ZBTB46 Antibody (PCRP-ZBTB46-2B8) - Azide and BSA Free

• BTB (POZ) domain containing 4
• BTB/POZ domain-containing protein 4
• BTBD4
• dJ583P15.7
• dJ583P15.8
• FLJ13502
• RINZF
• zinc finger and BTB domain containing 46
• zinc finger and BTB domain-containing protein 46
• Zinc finger protein 340
• ZNF340

Background

ZBTB46 may be involved in transcriptional regulation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBTB46