YM1/Chitinase 3-like 3 Antibody (281926) [Unconjugated]

Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse YM1/Chitinase 3-like 3 Monoclonal Antibody (Catalog # MAB2446) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 281926
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

YM1/Chitinase 3-like 3 Antibody (281926) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like
  Tyr22-Tyr398
  Accession # O35744
• Specificity: Detects mouse YM1/Chitinase 3-like in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Chil3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for YM1/Chitinase 3-like 3 Antibody (281926) [Unconjugated]

• AI505981
• CHI3L3
• Chitinase 3 like 3
• Chitinase 3-like 3
• ECF-L
• YM1

Background

Eosinophil Chemotactic Factor L (ECF-L), also known as YM1, is a secreted lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins but lacks enzymatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for YM1/Chitinase 3-like 3 Antibody (MAB2446)(6)

Publications using MAB2446

• Liu H, Zhang L, Liu Z et al. Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization iScience 2023-09-15 [PMID: 37636061]
• MOROKI T, MATSUO S, HATAKEYAMA H et al. Databases for technical aspects of immunohistochemistry-2021 update Journal of Toxicologic Pathology 2/24/2021 [PMID: 33976473]
• Stouch A, Zaynagetdinov R, Barham W, Stinnett A, Slaughter J, Yull F, Hoffman H, Blackwell T, Prince L IkappaB kinase activity drives fetal lung macrophage maturation along a non-M1/M2 paradigm. J Immunol, 2014-06-30;193(3):1184-93. 2014-06-30 [PMID: 24981452] (IHC, Mouse)
• Hardison S, Herrera G, Young M, Hole C, Wozniak K, Wormley F Protective immunity against pulmonary cryptococcosis is associated with STAT1-mediated classical macrophage activation. J Immunol, 2012-09-14;189(8):4060-8. 2012-09-14 [PMID: 22984078] (IHC-Fr, Mouse)
• Aldrich A, Kielian T Central nervous system fibrosis is associated with fibrocyte-like infiltrates. Am. J. Pathol., 2011-10-18;179(6):2952-62. 2011-10-18 [PMID: 22015460] (Western Blot, Mouse)
• Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia. Am. J. Pathol., 2008-12-18;174(1):153-63. 2008-12-18 [PMID: 19095958] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Chil3
• Uniprot:
  • O35744()