Wnt-9b Antibody (964742) [Unconjugated]

Recombinant Mouse Wnt‑9b (Catalog # 3669-WN) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse Wnt‑9b (400 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Wnt‑9b Monoclonal Antibody (Catalog # MAB36691). The ND50 is typically 125-750 ng/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 964742
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Wnt-9b Antibody (964742) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Wnt-9b
  Met1-Arg359
  Accession # O35468
• Specificity: Detects mouse Wnt-9b in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 125-750 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-9b Antibody (964742) [Unconjugated]

• Protein Wnt-14b
• wingless-type MMTV integration site family, member 15
• wingless-type MMTV integration site family, member 9B
• Wnt-14b
• WNT14Bprotein Wnt-9b
• Wnt-15
• WNT15Protein Wnt-15
• Wnt9b
• Wnt-9b

Background

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5‑7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

1. http://www.stanford.edu/group/nusselab/cgi-bin/wnt/.
2. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
3. Kirikoshi, H. and  M. Katoh (2002) Int. J. Mol. Med. 9:135.
4. Qian, J. et al. (2003) Genomics 81:34.
5. Carroll, T. J. et al. (2005) Dev. Cell 9:283.
6. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
7. Park, J-S. et al. (2007) Development 134:2533.
8. Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
9. Juriloff, D. M. and  M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O35468(Mouse)