| Reactivity | HuSpecies Glossary |
| Applications | Flow |
| Clone | 326711 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 405 |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | IC3157U-100UG | |||
| Alexa Fluor 488 | IC3157G-100UG | |||
| Alexa Fluor 594 | IC3157T-100UG | |||
| Alexa Fluor 647 | IC3157R-100UG | |||
| Alexa Fluor 700 | IC3157N-100UG | |||
| Alexa Fluor 750 | IC3157S-100UG | |||
| Unconjugated | MAB3157 | |||
| Immunogen | E. coli-derived recombinant human Wnt‑9a Pro48-Glu92 & Arg231-Phe304 Accession # O14904.2 |
| Specificity | Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -3a, -6, -7a, -7b, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | WNT9A |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
| Preservative | 0.09% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Wnt-9a (Wingless related integration site family member 9a, formerly Wnt-14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL and gastric, pancreatic and breast cancer cells. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.
![Wnt-9a Antibody (326711) [Alexa Fluor® 405]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Wnt-9a Antibody (326711) [Alexa Fluor® 405]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | IC3157U | |
| Alexa Fluor 405 | IC3157V | |
| Alexa Fluor 488 | IC3157G | |
| Alexa Fluor 594 | IC3157T | |
| Alexa Fluor 647 | IC3157R | |
| Alexa Fluor 700 | IC3157N | |
| Alexa Fluor 750 | IC3157S |
PTMs for Wnt-9a Antibody (IC3157V)Learn more about PTMs related to Wnt-9a Antibody (IC3157V).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3.](http://images.novusbio.com/images2/Wnt3 Antibody (1G7)-Western Blot-NBP2-46367-img0008.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0007.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0001.jpg)
![Western Blot: Wnt-2b Antibody (JB48-34) [NBP2-75713] - Analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.](http://images.novusbio.com/images2/Wnt-2b Antibody (JB48-34)-Western Blot-NBP2-75713-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Wnt-2b Antibody (JB48-34) [NBP2-75713] - Staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Wnt-2b-Antibody-(JB48-34)-Immunocytochemistry-Immunofluorescence-NBP2-75713-img0004.jpg)
![Immunohistochemistry-Paraffin: Wnt-2b Antibody (JB48-34) [NBP2-75713] - Analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Wnt-2b-Antibody-(JB48-34)-Immunohistochemistry-Paraffin-NBP2-75713-img0007.jpg)
![Western Blot: Wnt-7b Antibody [NBP1-59564] - Titration: 0.2-1 ug/ml, Positive Control: Human kidney.](http://images.novusbio.com/images2/Wnt-7b-Antibody-Western-Blot-NBP1-59564-img0007.jpg)
![Immunohistochemistry-Paraffin: Wnt-7b Antibody [NBP1-59564] - Human Brain, cortex tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/images2/Wnt-7b-Antibody-Immunohistochemistry-Paraffin-NBP1-59564-img0006.jpg)
![Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/beta-Catenin-Antibody-(12F7)-Western-Blot-NBP1-54467-img0008.jpg)
![Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY licence.](http://images.novusbio.com/images2/beta-Catenin-Antibody-(12F7)-Western-Blot-NBP1-54467-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).](http://images.novusbio.com/images2/beta-Catenin-Antibody-(12F7)-Immunocytochemistry-Immunofluorescence-NBP1-54467-img0004.jpg)
![Western Blot: Wnt-10a Antibody [NBP1-76916] - RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/images2/Wnt-10a-Antibody-Western-Blot-NBP1-76916-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Wnt-10a Antibody [NBP1-76916] - Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 ug/mL.](http://images.novusbio.com/images2/Wnt-10a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76916-img0003.jpg)
![Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP1-76916] - Staining of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 ug/ml.](http://images.novusbio.com/images2/Wnt-10a-Antibody-Western-Blot-NBP1-76916-img0004.jpg)
![Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Western-Blot-NBP1-51575-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Immunocytochemistry-Immunofluorescence-NBP1-51575-img0006.jpg)
![Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of human LAdrenal tissues using WNT1 mouse mAb.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Immunohistochemistry-Paraffin-NBP1-51575-img0007.jpg)
![Western Blot: Wnt-7a Antibody [NBP2-20913] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT7A antibody diluted at 1:5000.](http://images.novusbio.com/images2/Wnt-7a-Antibody-Western-Blot-NBP2-20913-img0008.jpg)
![Immunohistochemistry-Paraffin: Wnt-7a Antibody [NBP2-20913] - Paraffin-embedded mouse stomach. WNT7A antibody dilution: 1:500.](http://images.novusbio.com/images2/Wnt-7a-Antibody-Immunohistochemistry-Paraffin-NBP2-20913-img0006.jpg)
![Immunohistochemistry-Paraffin: Wnt-7a Antibody [NBP2-20913] - Paraffin-embedded mouse pancreas. WNT7A antibody dilution: 1:500.](http://images.novusbio.com/images2/Wnt-7a-Antibody-Immunohistochemistry-Paraffin-NBP2-20913-img0005.jpg)