Wnt-2b Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Wnt-2b Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant mouse Wnt‑2b
  Leu64-Glu104 and Arg251-Ala312
  Accession # O70283
• Specificity: Detects recombinant human and recombinant mouse Wnt-2b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-2 is observed and less than 5% cross-reactivity with rhWnt-
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-2b Antibody [Alexa Fluor® 647]

• Protein Wnt-13
• protein Wnt-2b
• wingless-type MMTV integration site family, member 2B
• Wnt-13
• WNT13XWNT2, Xenopus, homolog of
• Wnt2b
• Wnt-2b
• XWNT2
• XWNT2wingless-type MMTV integration site family, member 13

Background

Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kDa member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules. Wnt proteins are associated with developmental and carcinogenic processes. Wnt-2b may be necessary to maintain stem cells in an undifferentiated state. As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form. In the region from aa 64‑104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251‑312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.