WHSC1 Antibody

Product Discontinued

WHSC1 Antibody Summary

• Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_001035889.1; NP_579877.1; NP_579878.1; NP_579890.1; NP_579889.1; NP_015627.1.
• Epitope: EFSIKQSPLSVQS-C
• Localization: Nuclear
• Specificity: This antibody is expected to recognise all reported human isoforms. NP_001035889.1, NP_579877.1, NP_579878.1 and NP_579890.1 encode the same isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NSD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:64000
  • Western Blot 1:1000
• Application Notes: In WB this antibody has been successfully used on Human glioma tissues showing a single 30kDa band and no band was seen in normal brain tissues. Besides, the 30kDa was blocked by siRNA when transfected into several human glioma cell lines: Li et al, Neuro Oncol. 2008 Feb;10(1):45-51. PMID: 18182627. IHC: This antibody showed nuclear staining in human glioma cells exclusively: Li et al, Neuro Oncol. 2008 Feb;10(1):45-51. PMID: 18182627.

Reactivity Notes

Cross reacts with Human. Expected from sequence similarity: Dog.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WHSC1 Antibody

• EC 2.1.1.43
• FLJ23286
• IL5 promoter REII region-binding protein
• KIAA1090
• MGC176638
• MMSETNSD2Multiple myeloma SET domain-containing protein
• multiple myeloma SET domain containing protein type III
• nuclear receptor binding SET domain protein 2
• Nuclear SET domain-containing protein 2
• probable histone-lysine N-methyltransferase NSD2
• Protein trithorax-5
• REIIBP
• trithorax/ash1-related protein 5
• TRX5
• WHS
• Wolf-Hirschhorn syndrome candidate 1 protein
• Wolf-Hirschhorn syndrome candidate 1

Background

This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WHSC1 Antibody (NB100-1141)(1)

Publication using NB100-1141

• Li J, Yin C, Okamoto H et al. Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. Neuro Oncol 2008-01-08 [PMID: 18182627] (IHC-P, WB, Human)

Bioinformatics

• Gene Symbol: NSD2
• Entrez:
  • 7468(Human)
• Uniprot:
  • NP_015627(Human)