WDFY3 Antibody - BSA Free

Western Blot: WDFY3 Antibody [NBP1-03332] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-03332 used for WB at 1 mcg/ml (A and B) and used for IP at 3 mcg/mg lysate.

Immunocytochemistry/Immunofluorescence: WDFY3 Antibody [NBP1-03332] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-WDFY3 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: WDFY3 Antibody [NBP1-03332] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human glioblastoma using 1:200 dilution of WDFY3 antibody (NBP1-03332). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin

Product Discontinued

WDFY3 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal portion of the human WDFY3 protein (between amino acids 500-550) [Q8IZQ1]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WDFY3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Electron Microscopy reported in scientific literature (PMID 24358206) Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24664425). Immunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27330028).

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WDFY3 Antibody - BSA Free

• ALFY
• Autophagy-linked FYVE protein
• KIAA0993MGC16461
• WD repeat and FYVE domain containing 3
• WD repeat and FYVE domain-containing protein 3
• ZFYVE25

Background

The WDFY3 gene encodes a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WDFY3 Antibody (NBP1-03332)(8)

Publications using NBP1-03332

• Karanasios E, Ktistakis NT. Studying Autophagy: List of Useful Antibodies Produced via a Community Effort. Autophagy at the Cell, Tissue and Organismal Level. 2016-04-24
• Soreng K, Pankiv S, Bergsmark C et al. ALFY localizes to early endosomes and cellular protrusions to facilitate directional cell migration Journal of cell science 2022-02-15 [PMID: 35099014] (WB)
• Wu DJ, Adamopoulos IE. Loss of WDFY3 ameliorates severity of serum transfer-induced arthritis independently of autophagy. Cell Immunol 2017-04-22 [PMID: 28449847]
• Suzuki E, Maverakis E, Sarin R et al. T Cell-Independent Mechanisms Associated with Neutrophil Extracellular Trap Formation and Selective Autophagy in IL-17A-Mediated Epidermal Hyperplasia. J. Immunol. 2016-10-24 [PMID: 27798153] (ICC/IF, WB)
• Wu DJ, Gu R, Sarin R et al. Autophagy-linked FYVE containing protein WDFY3 interacts with TRAF6 and modulates RANKL-induced osteoclastogenesis. J. Autoimmun. 2016-06-18 [PMID: 27330028] (IP, ICC/IF, WB, Mouse)
• Orosco LA, Ross AP, Cates SL et al. Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology. Nat Commun. 2014-09-08 [PMID: 25198012]
• Park S, Jang I, Zuber C et al. ERADication of EDEM1 occurs by selective autophagy and requires deglycosylation by cytoplasmic peptide N-glycanase. Histochem. Cell Biol. 2014-03-25 [PMID: 24664425] (EM, ICC/IF, Human)
• Sommi P, Necchi V, Vitali A et al. PaCS Is a Novel Cytoplasmic Structure Containing Functional Proteasome and Inducible by Cytokines/Trophic Factors. PLoS One 2013-12-17 [PMID: 24358206] (EM, Human)

Review for WDFY3 Antibody (NBP1-03332) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IP Mouse 03/09/2015

Summary

• Application: Immunoprecipitation
• Species: Mouse
• Lot: A2

FAQs for WDFY3 Antibody (NBP1-03332). (Showing 1 - 2 of 2 FAQs).

1. I am now trying to detect WDFY3 by Western blot with your antibody NBP1-03332. Do you have any idea about the positive control for it?
   • We recommend using HeLa, 293T or Jurkat cell lysate as a positive control and loading between 25-50ug of protein per well.
2. I was wondering what percentage gel was used to resolve the Wdfy3 NBP1-03332 protein by western blot?
   • The image on our website is a western blot using a gradient gel that was 3-8% tris-acetate buffer.

Bioinformatics

• Gene Symbol: WDFY3
• Entrez:
  • 23001(Human)
• Uniprot:
  • Q8IZQ1(Human)