Western Blot: WDFY3 Antibody [NBP1-03332] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-03332 used for WB at 1 mcg/ml (A and B) and used for IP ...read more
Immunohistochemistry-Paraffin: WDFY3 Antibody [NBP1-03332] - Sample: FFPE section of human glioblastoma. Antibody: Affinity purified rabbit anti-fs18 WDFY3 fs20 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunocytochemistry/Immunofluorescence 1:500 to 1:2500
Immunoprecipitation 2-5 mcg/mg lysate
Use in Electron Microscopy reported in scientific literature (PMID 24358206) Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24664425). Immunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections
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