WDFY3 Antibody

Images

 
Western Blot: WDFY3 Antibody [NBP1-03332] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-03332 used for WB at 1 mcg/ml (A and B) and used for IP ...read more
Immunohistochemistry-Paraffin: WDFY3 Antibody [NBP1-03332] - Sample: FFPE section of human glioblastoma. Antibody: Affinity purified rabbit anti-fs18 WDFY3 fs20 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

WDFY3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human WD repeat and FYVE domain containing 3 using the numbering given in entry NP_055806.2 (GeneID 23001).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WDFY3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence 1:500 to 1:2500
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-5 mcg/mg lysate
Application Notes
Use in Electron Microscopy reported in scientific literature (PMID 24358206) Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24664425). Immunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections
Reviewed Applications
Read 1 Review rated 5
using
NBP1-03332 in the following applications:

Publications
Read Publications using
NBP1-03332 in the following applications:

  • EM
    2 publications
  • 3 publications
  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WDFY3 Antibody

  • ALFY
  • Autophagy-linked FYVE protein
  • KIAA0993MGC16461
  • WD repeat and FYVE domain containing 3
  • WD repeat and FYVE domain-containing protein 3
  • ZFYVE25

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00008878-M01
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
NBP2-02627
Species: Hu, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-37399
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
NBP1-19151
Species: Hu, Ma
Applications: WB, IHC-P, IP
NBP1-85091
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00002965-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
NBP2-03024
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-30382
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82016
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15501
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-71703
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-56139
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-85724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-56928
Species: Hu
Applications: WB, ICC/IF
NB110-53818
Species: Hu, Mu, Rt, Po, Bv, Pm, Xp, Ze
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, PLA
NBP2-48706
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-60928
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, EM, IHC, IHC-P
NB100-56343
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, IP

Publications for WDFY3 Antibody (NBP1-03332)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: EM, ICC/IF, IP, WB.


Filter By Application
EM
(2)
ICC/IF
(3)
IP
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-03332 Applications Species
Wu DJ, Adamopoulos IE. Loss of WDFY3 ameliorates severity of serum transfer-induced arthritis independently of autophagy. Cell. Immunol. Apr 22 2017 [PMID: 28449847]
Suzuki E, Maverakis E, Sarin R et al. T Cell-Independent Mechanisms Associated with Neutrophil Extracellular Trap Formation and Selective Autophagy in IL-17A-Mediated Epidermal Hyperplasia. J. Immunol. Oct 24 2016 [PMID: 27798153] (WB, ICC/IF) WB, ICC/IF
Wu DJ, Gu R, Sarin R et al. Autophagy-linked FYVE containing protein WDFY3 interacts with TRAF6 and modulates RANKL-induced osteoclastogenesis. J. Autoimmun. Jun 18 2016 [PMID: 27330028] (IP, WB, ICC/IF, Mouse) IP, WB, ICC/IF Mouse
Orosco LA, Ross AP, Cates SL et al. Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology. Nat Commun. 2014 Sep 09 [PMID: 25198012]
Park S, Jang I, Zuber C et al. ERADication of EDEM1 occurs by selective autophagy and requires deglycosylation by cytoplasmic peptide N-glycanase. Histochem. Cell Biol. 3/25/2014 [PMID: 24664425] (ICC/IF, EM, Human) ICC/IF, EM Human
Sommi P, Necchi V, Vitali A et al. PaCS Is a Novel Cytoplasmic Structure Containing Functional Proteasome and Inducible by Cytokines/Trophic Factors. PLoS One 2013 Dec 17 [PMID: 24358206] (EM, Human) EM Human
Karanasios E, Ktistakis NT. Studying Autophagy: List of Useful Antibodies Produced via a Community Effort. Autophagy at the Cell, Tissue and Organismal Level. 2016 Apr 24

Review for WDFY3 Antibody (NBP1-03332) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IP.

Reviews using NBP1-03332:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation WDFY3 NBP1-03332
Enlarge
reviewed by:
Anonymous
IP Mouse 03/09/2015
View

Summary

ApplicationImmunoprecipitation
SpeciesMouse
LotA2
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WDFY3 Antibody (NBP1-03332). (Showing 1 - 2 of 2 FAQs).

  1. I am now trying to detect WDFY3 by Western blot with your antibody NBP1-03332. Do you have any idea about the positive control for it?
    • We recommend using HeLa, 293T or Jurkat cell lysate as a positive control and loading between 25-50ug of protein per well.
  2. I was wondering what percentage gel was used to resolve the Wdfy3 NBP1-03332 protein by western blot?
    • The image on our website is a western blot using a gradient gel that was 3-8% tris-acetate buffer.

Secondary Antibodies

 

Isotype Controls

Additional WDFY3 Products

Bioinformatics Tool for WDFY3 Antibody (NBP1-03332)

Discover related pathways, diseases and genes to WDFY3 Antibody (NBP1-03332). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for WDFY3 Antibody (NBP1-03332)

Discover more about diseases related to WDFY3 Antibody (NBP1-03332).
 

Pathways for WDFY3 Antibody (NBP1-03332)

View related products by pathway.

PTMs for WDFY3 Antibody (NBP1-03332)

Learn more about PTMs related to WDFY3 Antibody (NBP1-03332).

Blogs on WDFY3

There are no specific blogs for WDFY3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
03/09/2015
Application: IP
Species: Mouse

Bioinformatics

Gene Symbol WDFY3
Entrez
Uniprot