VTI1B Antibody

Western Blot: VTI1B Antibody [NB100-2835] - (1ug/ml) analysis of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VTI1B Antibody Summary

• Description: Novus Biologicals Goat VTI1B Antibody (NB100-2835) is a polyclonal antibody validated for use in WB and ELISA. Anti-VTI1B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RDFDEKQQEANET corresponding to internal region according to NP_006361.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VTI1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 26-28 kDa band observed in human duodenum, kidney and testis lysates (calculated MW of 26.7 kDa band according to NP_006361.1).
• Control:

  VTI1B Overexpression Lysate
  Human Duodenum Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VTI1B Antibody

• vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
• Vesicle transport v-SNARE protein Vti1-like 1
• vesicle-associated soluble NSF attachment protein receptor
• v-SNARE
• VTI1
• VTI1L
• VTI1L1
• VTI1-LIKE
• vti1-rp1
• VTI2vesicle transport through interaction with t-SNAREs homolog 1B

Background

VTI1B mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. It may be concerned with increased secretion of cytokines associated with cellular senescence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VTI1B Antibody (NB100-2835)(1)

Publication using NB100-2835

• Murray RZ, Wylie FG, Khromykh T et al. Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha. J Biol Chem 2005-03-18 [PMID: 15640147]

Bioinformatics

• Gene Symbol: VTI1B
• Entrez:
  • 10490(Human)
  • 53612(Mouse)
• Uniprot:
  • NP_006361.1(Human)