VPS35 Antibody (JB33-82)

Knockout Validated: VPS35 Antibody (JB33-82) [NBP2-75710] - Lysates of HAP1 WT and VPS35 KO were prepared, and 20 ug of protein were processed for immunoblot with the VPS35 antibody (JB33-82). The Ponceau stained transfer of the blot is shown. Antibody dilution used: NBP2-75710 at 1/1000. Predicted band size: 91 kDa. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.Positive control:Lane 1: PC-3M Lane 2: U937Lane 3: SH-SY-5Y

Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

Flow Cytometry: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.

Product Discontinued

VPS35 Antibody (JB33-82) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human VPS35 aa 80-220 / 796. (SwissProt: Q96QK1 Human; SwissProt: Q9EQH3 Mouse)
• Localization: Cytoplasm. Membrane. Endosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VPS35
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:300
  • Knockout Validated
  • Western Blot 1:500-1:1000
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for VPS35 Antibody (JB33-82)

• DKFZp434P1672
• FLJ10752
• FLJ13588
• FLJ20388
• hVPS35
• Maternal-embryonic 3
• MEM3DKFZp434E1211
• vacuolar protein sorting 35 (yeast homolog)
• vacuolar protein sorting 35 (yeast)
• vacuolar protein sorting 35 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 35
• Vesicle protein sorting 35

Background

The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS35