VPS18 Antibody - BSA Free

Western Blot: VPS18 Antibody [NBP3-12906] - VPS18 antibody detects VPS18 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS18 antibody (NBP3-12906) diluted at a dilution of 1:1000.

Immunocytochemistry/Immunofluorescence: VPS18 Antibody [NBP3-12906] - VPS18 antibody detects VPS18 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS18 protein stained by VPS18 antibody (NBP3-12906) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VPS18 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS18. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Rat (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS18
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for VPS18 Antibody - BSA Free

• hVPS18
• KIAA1475PEP3
• vacuolar protein sorting 18 homolog (S. cerevisiae)
• vacuolar protein sorting protein 18
• vacuolar protein sorting-associated protein 18 homolog

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS18