VPS11 Antibody

Images

 
Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - (4ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - 3.8ug/ml staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VPS11 Antibody Summary

Immunogen
Peptide with sequence C-HALQEKDTQTKLE corresponding to internal region according to NP_068375.3.
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS11
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in human duodenum, colon and placenta lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108 kDa band according to NP_068375.3. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows brushborder staning in PCT cells and human pancreas shows cytoplasm staining of beta cells in islets of langerhans.
Control
VPS11 Overexpression Lysate
Publications
Read Publication using NB100-2412.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS11 Antibody

  • hVPS11
  • PEP5
  • RING finger protein 108
  • RNF108END1
  • vacuolar protein sorting 11 (yeast homolog)
  • vacuolar protein sorting 11 homolog (S. cerevisiae)
  • vacuolar protein sorting protein 11
  • vacuolar protein sorting-associated protein 11 homolog

Background

VPS11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS11 Antibody (NB100-2412)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol VPS11
Entrez
Uniprot