VKORC1 Overexpression Lysate (Native)

Images

 
Western Blot: VKORC1 Overexpression Lysate (Adult Normal) [NBL1-17727] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VKORC1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

VKORC1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202639 and based on accession number NM_024006. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens vitamin K epoxide reductase complex, subunit 1 (VKORC1), transcript variant 1, mRNA.
Gene
VKORC1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for VKORC1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for VKORC1 Overexpression Lysate (Native)

  • EC 1.1.4.1
  • EDTP308
  • FLJ00289
  • IMAGE3455200
  • MST576
  • phylloquinone epoxide reductase
  • vitamin K dependent clMST134
  • vitamin K epoxide reductase complex subunit 1
  • vitamin K epoxide reductase complex, subunit 1
  • Vitamin K1 2,3-epoxide reductase subunit 1
  • vitamin K1 epoxide reductase (warfarin-sensitive)
  • VKCFD2
  • VKORMGC2694

Background

Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. The product of this gene encodes the enzyme that is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. Two pseudogenes have been identified on chromosome 1 and the X chromosome. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the predominant isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-01397
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-86461
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-16651
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-58268
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-90348
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-43581
Species: Hu
Applications: WB, ICC/IF, IP
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP1-85488
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-76544
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P
NBP1-88866
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-45478
Species: Hu, Mu, Rt, Mk
Applications: WB, IHC
MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
NBP2-02356
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-07799
Species: Hu
NBP2-37502
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready

Publications for VKORC1 Lysate (NBL1-17727) (0)

There are no publications for VKORC1 Lysate (NBL1-17727).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VKORC1 Lysate (NBL1-17727) (0)

There are no reviews for VKORC1 Lysate (NBL1-17727). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VKORC1 Lysate (NBL1-17727) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional VKORC1 Products

Bioinformatics Tool for VKORC1 Lysate (NBL1-17727)

Discover related pathways, diseases and genes to VKORC1 Lysate (NBL1-17727). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VKORC1 Lysate (NBL1-17727)

Discover more about diseases related to VKORC1 Lysate (NBL1-17727).
 

Pathways for VKORC1 Lysate (NBL1-17727)

View related products by pathway.

PTMs for VKORC1 Lysate (NBL1-17727)

Learn more about PTMs related to VKORC1 Lysate (NBL1-17727).

Blogs on VKORC1

There are no specific blogs for VKORC1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

VKORC1 Antibody
NBP1-92573

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VKORC1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol VKORC1