VISTA/B7-H5/PD-1H Antibody (CL3975) - Azide and BSA Free

Analysis in human lymph node and testis tissues using NBP3-44112 antibody. Corresponding VISTA/B7-H5/PD-1H RNA-seq data are presented for the same tissues.

Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.

Staining of human lymph node shows moderate to strong cytoplasmic positivity in non-germinal center cells.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Analysis in human cell line CAPAN-2.

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3975
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VISTA/B7-H5/PD-1H Antibody (CL3975) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse VISTA/B7-H5/PD-1H Antibody (CL3975) - Azide and BSA Free (NBP2-59030) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9H7M9, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VSIR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for VISTA/B7-H5/PD-1H Antibody (CL3975) - Azide and BSA Free

• 4632428N05Rik
• B7H5
• B7-H5
• C10orf54
• chromosome 10 open reading frame 54
• Dies1
• Gi24
• PD1H
• PD-1H
• platelet receptor Gi24
• PP2135
• SISP1
• stress induced secreted protein 1
• VISTA
• VSIR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VSIR