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VIPR1/VPAC1 Antibody - BSA Free

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Immunohistochemistry-Paraffin: VIPR1/VPAC1 Antibody [NLS1297] - Analysis of anti-VIP Receptor 1 antibody with human adipocytes.
Immunohistochemistry-Paraffin: VIPR1/VPAC1 Antibody [NLS1297] - Analysis of anti-VIP Receptor 1 antibody with human adipocytes.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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VIPR1/VPAC1 Antibody - BSA Free Summary

Immunogen
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human VIPR1/VPAC1.
Epitope
3rd cytoplasmic domain
Localization
Plasma membrane
Specificity
Human VIPR1/VPAC1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VIPR1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (88%), Rabbit (88%), Mouse (81%), Rat (81%), Bovine (81%), Guinea pig (81%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VIPR1/VPAC1 Antibody - BSA Free

  • FLJ41949
  • HVR1
  • II
  • PACAP type II receptor
  • PACAP-R2
  • RDC1
  • type II
  • V1RG
  • vasoactive intestinal peptide receptor 1
  • vasoactive intestinal polypeptide receptor 1
  • VIP R1
  • VIP receptor, type I
  • VIPR
  • VIPR1
  • VPAC1
  • VPAC1R

Background

Vasoactive Intestinal Polypeptide Receptor 1 (VIPR1) is a member of the Vasoactive Intestinal Polypeptide subfamily. Vasoactive intestinal peptide (VIP) plays multiple roles in the nervous, endocrine, and immune systems as a neurotransmitter, a hormone, and a cytokine. VIPR1, a receptor for VIP, has been shown to expressed widely in normal tissues and is the predominant receptor subtype for VIP in cancers. VIP and its receptors, VIPR1 and VIPR2, are highly expressed in the immune system and modulate diverse T cell functions. Recently VIP-R1 has been shown to be a novel and potent facilitator of HIV-1 infection. Two isoforms are produced by alternative splicing. VIPR1 expression has been documented widely in normal and cancerous tissues, including adipose, adrenal, bladder, blood, brain, breast, colon, ganglion, GI tract, heart, kidney, liver, lung, lymph node, ovary, pancreas, placenta, prostate, skin, small intestine, spinal cord, spleen, stomach, testis, thymus, thyroid, and vessel. ESTs have been isolated from a diverse set of normal and cancerous tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications: IHC, WB
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Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PACAP/ADCYAP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2
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VIP R2 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 25 µg/mL Mouse Anti-Human VIP R2 Monoclonal Antibody (Catalog # MAB5416) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-92287] - Analysis using Anti-MZB1 antibody NBP1-92287 (A) shows similar pattern to independent antibody NBP2-55874 (B).
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-92287] - Staining of human liver, lymph node, placenta and small intestine using Anti-MZB1 antibody NBP1-92287 (A) shows similar protein distribution across tissues to independent antibody NBP2-55874 (B).
Immunohistochemistry-Paraffin: Proapoptotic Caspase Adaptor Protein Antibody [NBP1-92287] - Analysis in human lymph node and liver tissues using NBP1-92287 antibody. Corresponding MZB1 RNA-seq data are presented for the same tissues.
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Western Blot: PAC1R Antibody [NBP2-47340] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340] - Staining of human pancreas shows low expression as expected.
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Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
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Immunocytochemistry/Immunofluorescence: PSMG1 Antibody [NBP1-36951] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Immunocytochemistry/Immunofluorescence: PSMG1 Antibody [NBP1-36951] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Golgi apparatus and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
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Applications: Flow, ICC/IF, PEP-ELISA, WB
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Western Blot: DUSP2 Antibody [NBP2-92318] - Analysis of extracts of various cell lines, using DUSP2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Butyrylcholinesterase/ ...
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MASA Antibody
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Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-54301] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257)
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-54301] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-54301] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
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CXCR4 Antibody
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CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND50 is typically 2.5-12 µg/mL.
CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Bioinformatics

Gene Symbol VIPR1
Entrez
Uniprot